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6-Azauracil

Product Name
6-Azauracil
CAS No.
461-89-2
Chemical Name
6-Azauracil
Synonyms
1,2,4-TRIAZINE-3,5(2H,4H)-DIONE;1,2,4-triazine-3,5-diol;Azauracil;AZU;6-AU;hHBP;NAZC;AZAMP;ipo3834;nsc3425
CBNumber
CB9223189
Molecular Formula
C3H3N3O2
Formula Weight
113.07
MOL File
461-89-2.mol
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6-Azauracil Property

Melting point:
274-275 °C(lit.)
Boiling point:
211.75°C (rough estimate)
Density 
1.5988 (rough estimate)
refractive index 
1.4264 (estimate)
storage temp. 
Store below +30°C.
solubility 
1 M NH4OH: 50mg/mL, clear to slightly hazy, colorless to light yellow-green
pka
7.78±0.20(Predicted)
form 
Fine Crystalline Powder
color 
White to light yellow
Water Solubility 
Partly soluble in water, ethanol, DMSO, and 1 M NH4OH (50 mg/ml).
BRN 
116472
InChI
InChI=1S/C3H3N3O2/c7-2-1-4-6-3(8)5-2/h1H,(H2,5,6,7,8)
InChIKey
SSPYSWLZOPCOLO-UHFFFAOYSA-N
SMILES
N1=CC(=O)NC(=O)N1
CAS DataBase Reference
461-89-2(CAS DataBase Reference)
NIST Chemistry Reference
1,2,4-Triazine-3,5(2H,4H)-dione(461-89-2)
EPA Substance Registry System
1,2,4-Triazine-3,5(2H,4H)-dione (461-89-2)
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Safety

Hazard Codes 
Xi,T
Risk Statements 
36/37/38-63-25
Safety Statements 
26-36-45-38-36/37/39
WGK Germany 
3
RTECS 
XY7700000
10-23
TSCA 
Yes
HazardClass 
IRRITANT
HS Code 
29336980
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H301Toxic if swalloed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
8.41156
Product name
6-Azauracil
Purity
for synthesis
Packaging
5G
Price
$88.1
Updated
2025/07/31
Sigma-Aldrich
Product number
A1757
Product name
6-Azauracil
Purity
≥98%
Packaging
5g
Price
$103
Updated
2025/07/31
Sigma-Aldrich
Product number
8.41156
Product name
6-Azauracil
Purity
for synthesis
Packaging
25g
Price
$385
Updated
2025/07/31
TCI Chemical
Product number
A0558
Product name
6-Azauracil
Purity
>99.0%(HPLC)(T)
Packaging
1g
Price
$18
Updated
2025/07/31
TCI Chemical
Product number
A0558
Product name
6-Azauracil
Purity
>99.0%(HPLC)(T)
Packaging
5g
Price
$49
Updated
2025/07/31
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6-Azauracil Chemical Properties,Usage,Production

Chemical Properties

white to light yellow fine crystalline powder

Uses

6-Azauracil (6-AU) is used as a growth inhibitor of various microorganisms via depletion of intracellular GTP and UTP nucleotide pools. 6-Azauracil has been widely used in investigations on modulation of transcription, especially in yeast models.

Definition

ChEBI: 6-azauracil is a 1,2,4-triazine compound having oxo-substituents at the 3- and 5-positions. It has a role as an antimetabolite. It is a member of 1,2,4-triazines and a nucleobase analogue.

Synthesis Reference(s)

The Journal of Organic Chemistry, 23, p. 1522, 1958 DOI: 10.1021/jo01104a033

Biochem/physiol Actions

6-Azauracil (6-AU) is a pyrimidine analog of uracil and exhibits antitumor activity. It inhibits the growth of various microorganisms by depleting intracellular guanosine triphosphate (GTP)?and uridine?triphosphate (UTP) nucleotide pools.

Safety Profile

Poison by intraperitoneal route. Experimental reproductive effects. Questionable carcinogen with experimental tumorigenic data. Mutation data reported. When heated to decomposition it emits toxic fumes of NOx.

Synthesis

928-73-4

461-89-2

The general procedure for the synthesis of 1,2,4-triazine-3,5(2H,4H)-dione from the compound (CAS: 928-73-4) was as follows: 2-[2-(aminocarbonyl)fluorenylidene] 33.9 g (0.259 mol) and 51 g (1.275 mol) of sodium hydroxide were accurately weighed and dissolved in 150 mL of ethylene glycol. The reaction mixture was stirred continuously for 12 hours at 80 °C using a magnetic stirrer. Upon completion of the reaction, the mixture was cooled to room temperature, 250 mL of methanol was added and allowed to stand for 12 hours to promote crystallization. Subsequent filtration was carried out to recover the solvent to give 41.9 g of wet product. The wet product was dissolved in 60 mL of ultrapure water and the pH of the solution was adjusted with concentrated hydrochloric acid to less than 3. The solution was heated to 80 °C and stirring was maintained for half an hour before it was rapidly cooled to 10 °C with ice brine. The precipitate was collected by filtration and washed well with ultrapure water. Finally, the product was dried under vacuum to give 18 g (0.159 mol, 61.6% yield) of 6-azauracil (CD-2).

Mode of action

6-Azauracil is a growth inhibitor effective on a variety of microorganisms. It functions by depleting the intracellular GTP and UTP nucleotide supply. Mutations in transcriptional elongation machinery, as well as mutations in a variety of other pathways, exaggerate the growth defect of cells in the presence of 6 AU.

References

[1] Patent: CN108440505, 2018, A. Location in patent: Paragraph 0089; 0102; 0103; 0104
[2] Journal of Organic Chemistry, 1958, vol. 23, p. 1522
[3] Chemische Berichte, 1947, vol. 80, p. 494,500
[4] Patent: US2003/69140, 2003, A1
[5] Journal of the Iranian Chemical Society, 2016, vol. 13, # 8, p. 1539 - 1547

6-Azauracil Preparation Products And Raw materials

Raw materials

Preparation Products

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6-Azauracil Suppliers

ITIC MEDCHEM(SUZHOU) CO.,LTD.
Tel
0512-68323967 18015559028
Fax
+86-512-69200360
Email
sales@iticmedchem.com
Country
China
ProdList
506
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61
Hebei Fanbo Pharmaceutical Technology Co., Ltd.
Tel
0531-0531-88163906-805-805 15588346797
Email
fanbomedical@163.com
Country
China
ProdList
564
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58
Chongqing Longsheng Pharmaceutical Co., Ltd
Tel
17791355738
Email
sales@cqlonghoo.com
Country
China
ProdList
94
Advantage
58
J & K SCIENTIFIC LTD.
Tel
18210857532; 18210857532
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
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76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
4006356688 18621169109
Fax
86-21-61259102
Email
market03@meryer.com
Country
China
ProdList
40228
Advantage
62
INTATRADE GmbH
Tel
+49 3493/605464
Fax
+49 3493/605470
Email
sales@intatrade.de
Country
Germany
ProdList
3572
Advantage
66
Alfa Aesar
Tel
400-6106006
Fax
021-67582001/03/05
Email
saleschina@alfa-asia.com
Country
China
ProdList
30123
Advantage
84
TAIYUAN RHF CO.,LTD.
Tel
+86 351 7031519
Fax
+86 351 7031519
Email
sales@RHFChem.com
Country
China
ProdList
2338
Advantage
56
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24529
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81
Energy Chemical
Tel
021-021-58432009 400-005-6266
Fax
021-58436166
Email
sales8178@energy-chemical.com
Country
China
ProdList
44801
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View Lastest Price from 6-Azauracil manufacturers

Unipharm pharmaceutical industry Co., Ltd
Product
6-Azauracil 461-89-2
Price
US $1.00/kg
Min. Order
1kg
Purity
98%
Supply Ability
1000kg
Release date
2023-03-23
Shaanxi Dideu Medichem Co. Ltd
Product
6-Azauracil 461-89-2
Price
US $1.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
20T
Release date
2020-05-14
Honest Joy Holdings Limited
Product
6-Azauracil 461-89-2
Price
US $0.00/KG
Min. Order
1KG
Purity
98.7%
Supply Ability
100 tons
Release date
2022-02-18

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  • BioChemical
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