ChemicalBook > CAS DataBase List > HEPES sodium salt

HEPES sodium salt

Product Name
HEPES sodium salt
CAS No.
75277-39-3
Chemical Name
HEPES sodium salt
Synonyms
HEPES NA;HEPES SODIUM SALT BUFFER;H7006-500G;(HEPES)2-NA;HEPES, SODIUM;HEPES NA-SALT;RUTHENIUM STAND;HEPES LOWSODIUM;HEPES SODIUM SALT;1.0 M HEPES sodium
CBNumber
CB9773992
Molecular Formula
C8H19N2NaO4S
Formula Weight
262.3
MOL File
75277-39-3.mol
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HEPES sodium salt Property

Melting point:
234 °C
Density 
1.504[at 20℃]
vapor pressure 
0Pa at 25℃
storage temp. 
room temp
solubility 
H2O: 1 M at 20 °C, clear, colorless
form 
dry powder
color 
White
PH Range
6.8 - 8.2
PH
10.0-12.0 (1M in H2O)
pka
7.5(at 25℃)
biological source
synthetic
Water Solubility 
It is soluble in water.
Sensitive 
Hygroscopic
Stability:
Stable. Hygroscopic - protect from moisture. Incompatible with strong oxidizing agents.
CAS DataBase Reference
75277-39-3(CAS DataBase Reference)
EPA Substance Registry System
1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, monosodium salt (75277-39-3)
Absorption
≤0.2 at 260 in H2O at 1M
≤0.2 at 280 in H2O at 1M
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Safety

Safety Statements 
22-24/25
WGK Germany 
3
F 
3-10
TSCA 
Yes
HS Code 
29335995
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
RES6007H-A7
Product name
HEPES sodium salt
Packaging
1kg
Price
$1141
Updated
2025/07/31
Sigma-Aldrich
Product number
RES6007H-A7
Product name
HEPES sodium salt
Packaging
25kg
Price
$14299
Updated
2025/07/31
Sigma-Aldrich
Product number
391333
Product name
HEPES, Sodium Salt, ULTROL® Grade
Purity
A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.55 at 25°C. Absorbance (1.0 mM, H2O, 260 nm):0.10.
Packaging
100G
Price
$166
Updated
2025/07/31
Sigma-Aldrich
Product number
391333
Product name
HEPES, Sodium Salt, ULTROL® Grade
Purity
A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.55 at 25°C. Absorbance (1.0 mM, H2O, 260 nm):0.10.
Packaging
500G
Price
$590
Updated
2025/07/31
Sigma-Aldrich
Product number
391333
Product name
HEPES, Sodium Salt, ULTROL® Grade
Purity
A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.55 at 25°C. Absorbance (1.0 mM, H2O, 260 nm):0.10.
Packaging
1KG
Price
$900
Updated
2025/07/31
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HEPES sodium salt Chemical Properties,Usage,Production

Chemical Properties

white powder

Uses

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Definition

ChEBI: Sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate is an organic sodium salt, a N-(2-hydroxyethyl)piperazine and a N-(sulfoalkyl)piperazine. It contains a 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate.

General Description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Flammability and Explosibility

Not classified

Biological Activity

Multi purpose buffer used in biological research.

Synthesis

103-76-4

1562-00-1

75277-39-3

The general procedure for the synthesis of N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt from N-hydroxyethylpiperazine and sodium 2-hydroxyethanesulfonate is as follows: magnetic stirring in a 70-mL fluoropolymer-lined steel autoclave equipped with internal thermocouples for monitoring the temperature of the liquid phase, a pressure gauge, a pressure-reducing valve, and an exhaust valve. First, amine feedstock, anhydrous or aqueous sodium 2-hydroxyethanesulfonate solution, and anhydrous or aqueous sodium hydroxide solution were added to the open-topped autoclave. Subsequently, the autoclave was assembled and immersed in an electrically heated oil bath. The stirred reaction mixture was rapidly heated to the specified reaction temperature with the exhaust valve closed and maintained at that temperature for the time specified in Examples 9, 10 and 13. Upon completion of the reaction, the mixture was cooled to room temperature, the reactor was opened, and the reaction product was dissolved in a sufficient amount of water to ensure complete dissolution of all solids.

storage

Room temperature

References

[1] Patent: US2006/89509, 2006, A1. Location in patent: Page/Page column 4-6
[2] Patent: US2006/89509, 2006, A1. Location in patent: Page/Page column 4-6

HEPES sodium salt Preparation Products And Raw materials

Raw materials

Preparation Products

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HEPES sodium salt Suppliers

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View Lastest Price from HEPES sodium salt manufacturers

Hebei Chuanghai Biotechnology Co., Ltd
Product
HEPES sodium salt 75277-39-3
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
500000kg
Release date
2024-11-01
Hebei Chuanghai Biotechnology Co,.LTD
Product
HEPES sodium salt 75277-39-3
Price
US $9.00/KG
Min. Order
1KG
Purity
99.8%
Supply Ability
100tons
Release date
2024-08-16
Suzhou Genemill Biotechnology Co.,Ltd
Product
HEPES NA 75277-39-3
Price
US $0.00/Kg
Min. Order
1Kg
Purity
99.5%
Supply Ability
1000tons
Release date
2023-09-14

75277-39-3, HEPES sodium saltRelated Search:

2-(METHYLSULFONYL)ETHANOL Piperazine citrate Sodium carbonate N-Aminoethylpiperazine 2-Hydroxyethanesulphonic acid Sodium benzoate Calcium dobesilate 2-Ethylpiperazine Sodium chloride Sodium bicarbonate Sodium isethionate Sodium formate HEPES 2-Piperidinoethanol Sodium acetate Hydroxyethyl starch Piperazine Ethanesulfonic acid

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