HEPES
- Product Name
- HEPES
- CAS No.
- 7365-45-9
- Chemical Name
- HEPES
- Synonyms
- HEPES BUFFER;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID;hepes solution;HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID;Biological Buffer;HEPES buffer Solution;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID;4-(2-hydroxyethyl)piperazin-1-ylethanesulphonic acid;2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID;EPES
- CBNumber
- CB5408557
- Molecular Formula
- C8H18N2O4S
- Formula Weight
- 238.3
- MOL File
- 7365-45-9.mol
HEPES Property
- Melting point:
- 234-238 °C
- Boiling point:
- 408℃[at 101 325 Pa]
- Density
- 1.07 g/mL at 20 °C
- vapor pressure
- 0Pa at 25℃
- refractive index
- n20/D 1.339
- storage temp.
- 2-8°C
- solubility
- H2O: 1 M at 20 °C, clear, colorless
- form
- powder
- color
- White
- PH Range
- 7.0 - 7.6
- PH
- 5.0-6.5 (1Maqueous solution)
- pka
- 7.5(at 25℃)
- Water Solubility
- Soluble
- λmax
- λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04 - Merck
- 14,4654
- BRN
- 883043
- Stability:
- Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
- InChIKey
- JKMHFZQWWAIEOD-UHFFFAOYSA-N
- LogP
- -3.85 at 20℃
- CAS DataBase Reference
- 7365-45-9(CAS DataBase Reference)
- EPA Substance Registry System
- 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)
Safety
- Hazard Codes
- Xi
- Risk Statements
- 36/37/38
- Safety Statements
- 24/25-22-36-26
- WGK Germany
- 1
- RTECS
- TL6809000
- TSCA
- Yes
- HS Code
- 29335995
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H302Harmful if swallowed
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P271Use only outdoors or in a well-ventilated area.
P280Wear protective gloves/protective clothing/eye protection/face protection.
N-Bromosuccinimide Price
- Product number
- PHG0001
- Product name
- HEPES
- Packaging
- 1KIT
- Price
- $978
- Updated
- 2024/03/01
- Product number
- H4034
- Product name
- HEPES
- Purity
- BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
- Packaging
- 25KG
- Price
- $6140
- Updated
- 2024/03/01
- Product number
- H3375
- Product name
- HEPES
- Purity
- ≥99.5% (titration)
- Packaging
- 25KG
- Price
- $5870
- Updated
- 2024/03/01
- Product number
- H3375
- Product name
- HEPES
- Purity
- ≥99.5% (titration)
- Packaging
- 50KG
- Price
- $11060
- Updated
- 2024/03/01
- Product number
- 83264
- Product name
- HEPES buffer solution
- Purity
- 1?M in H2O
- Packaging
- 100ML
- Price
- $67.1
- Updated
- 2024/03/01
HEPES Chemical Properties,Usage,Production
Description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Chemical Properties
White crystalline powder
Uses
Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.
Uses
HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
HEPES has been used as a component of:
Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.
Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.
Uses
HEPES is a biological buffer. It may be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for use with the Folin-Ciocalteu protein assay.
Definition
ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.
General Description
HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.
Biological Activity
HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
Biological Activity
Multi purpose buffer used in biological research.
storage
Room temperature
Purification Methods
Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]
HEPES Preparation Products And Raw materials
Raw materials
Preparation Products
HEPES Suppliers
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View Lastest Price from HEPES manufacturers
- Product
- 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid 7365-45-9
- Price
- US $0.00/Kg/Drum
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 200mt
- Release date
- 2021-11-24
- Product
- HEPES 7365-45-9
- Price
- US $0.00/kg
- Min. Order
- 1kg
- Purity
- 99%
- Supply Ability
- 2000ton
- Release date
- 2023-09-06
- Product
- HEPES 7365-45-9
- Price
- US $0.00/kg
- Min. Order
- 1kg
- Purity
- 99.5%
- Supply Ability
- 1000tons
- Release date
- 2023-09-14