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H-89 DIHYDROCHLORIDE HYDRATE

Product Name
H-89 DIHYDROCHLORIDE HYDRATE
CAS No.
130964-39-5
Chemical Name
H-89 DIHYDROCHLORIDE HYDRATE
Synonyms
H 89 2HCl;5-Isoquinolinesulfonamide;H-89 DIHYDROCHLORIDE HYDRATE;H-89 hydrate dihydrochloride;H-89 DIHYDROCHLORIDE HYDRATE USP/EP/BP;Protein kinase inhibitor H-89 dihydrochloride;H-89 Dihydrochloride Hydrate,N-[2-(P-Bromocinnamylamino)Et;H-89 dihydrochloride(Protein kinase inhibitor H-89 dihydrochloride);PROTEIN KINASE INHIBITOR H-89 DIHYDROCHLORIDE; H89 DIHYDROCHLORIDE;H 89;(E)-N-(2-(4-bromocinnamylamino)ethyl)isoquinoline-5-sulfonamide dihydrochloride
CBNumber
CB9972711
Molecular Formula
C20H20BrN3O2S.2ClH.H2O
Formula Weight
537.304
MOL File
130964-39-5.mol
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H-89 DIHYDROCHLORIDE HYDRATE Property

Melting point:
195-200°C
storage temp. 
Inert atmosphere,2-8°C
solubility 
H2O: ≥10mg/mL
form 
powder
color 
off-white
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Safety

Safety Statements 
22-24/25
WGK Germany 
3
HS Code 
2935909099
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
B1427
Product name
H-89 dihydrochloride hydrate
Purity
≥98% (HPLC), powder
Packaging
5mg
Price
$193
Updated
2024/03/01
Cayman Chemical
Product number
10010556
Product name
H-89 (hydrochloride)
Purity
≥98%
Packaging
5mg
Price
$49
Updated
2024/03/01
Cayman Chemical
Product number
10010556
Product name
H-89 (hydrochloride)
Purity
≥98%
Packaging
10mg
Price
$92
Updated
2024/03/01
Cayman Chemical
Product number
10010556
Product name
H-89 (hydrochloride)
Purity
≥98%
Packaging
25mg
Price
$216
Updated
2024/03/01
Cayman Chemical
Product number
10010556
Product name
H-89 (hydrochloride)
Purity
≥98%
Packaging
50mg
Price
$356
Updated
2024/03/01
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H-89 DIHYDROCHLORIDE HYDRATE Chemical Properties,Usage,Production

Chemical Properties

White Solid

Uses

H-89 dihydrochloride hydrate has been used in the inhibition of protein kinase A in Leydig cells, primary calvarial osteoblasts (pOBs), rat granulosa cell line (LH-15 cells) and human umbilical vein endothelial cells (HUVECs).

Uses

Selective inhibitor of Protein Kinase A (cyclic AMP-dependeant Protein Kinase) with an inhibitory constant of 0.0048 uM.

Definition

ChEBI: N-[2-(4-bromocinnamylamino)ethyl]isoquinoline-5-sulfonamide dihydrochloride is a hydrochloride salt prepared from N-[2-(4-bromocinnamylamino)ethyl]isoquinoline-5-sulfonamide and two equivalents of hydrogen chloride. It has a role as an EC 2.7.11.11 (cAMP-dependent protein kinase) inhibitor. It contains a N-[2-(4-bromocinnamylamino)ethyl]isoquinoline-5-sulfonamide(2+).

Biological Activity

h 89 2hcl is a potent pka inhibitor. in a cell-free assay, the ki of h 89 is 48 nm, 10-fold selective for pka than pkg and 500-fold greater selectivity than pkc, mlck, calmodulin kinase ii and casein kinase i/ii [1].[1]. chijiwa t, mishima a, hagiwara m, et al. inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic amp-dependent protein kinase, n-[2-(p-bromocinnamylamino) ethyl]-5-isoquinolinesulfonamide (h-89), of pc12d pheochromocytoma cells[j]. journal of biological chemistry, 1990, 265(9): 5267-5272.[2]. lochner a, moolman j a. the many faces of h89: a review[j]. cardiovascular drug reviews, 2006, 24(3‐4): 261-274.[3]. lee t h, linstedt a d. potential role for protein kinases in regulation of bidirectional endoplasmic reticulum-to-golgi transport revealed by protein kinase inhibitor h89[j]. molecular biology of the cell, 2000, 11(8): 2577-2590.

Biochem/physiol Actions

H-89 dihydrochloride hydrate is a selective inhibitor of protein kinase A (PKA). It also inhibits potassium (K+) current in rat myocytes. It mediates Na+ transport by interacting with α subunits of epithelial Na+ channel (ENaC).

in vitro

in pc12d cells, pretreatment with h-89 dose-dependently inhibited the forskolin-induced protein phosphorylation, with no influence in intracellular cyclic amp levels. in pc12d cells, h-89 significantly inhibited the forskolin-induced neurite outgrowth. in pc12d cells, pretreatment with h-89 (30 μm) strikingly inhibited camp-dependent histone iib phosphorylation activity in cell lysates while showed no effects on other protein phosphorylation activity such as cgmp-dependent histone iib phosphorylation activity [1]. h 89 was a potent and selective pka inhibitor with ki of 48 nm in a cell-free assay [2]. h89 also inhibited s6k1, msk1, pka, rockii, pkbα and mapkap-k1b kinases with ic50 of 80, 120, 135, 270, 2600 and 2800 nm, respectively [2]. in the hypotonic medium, 50 μm h89, a concentration commonly used to inhibit pka, prevented the redistribution response. in normal medium, h89 (50 μm) induced the redistribution of ergic 53 to the er by 20 min [3].

storage

-20°C

H-89 DIHYDROCHLORIDE HYDRATE Preparation Products And Raw materials

Raw materials

Preparation Products

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H-89 DIHYDROCHLORIDE HYDRATE Suppliers

ShangHai Biochempartner Co.,Ltd
Tel
17754423994 17754423994
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QQ:2853530913
Email
2853530910@QQ.com
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China
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J & K SCIENTIFIC LTD.
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010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
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Chembest Research Laboratories Limited
Tel
021-20908456
Fax
021-58180499
Email
sales@BioChemBest.com
Country
China
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Chemsky(shanghai)International Co.,Ltd.
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021-50135380
Email
shchemsky@sina.com
Country
China
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32344
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Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
sales@meilune.com
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China
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ShangHai YuanYe Biotechnology Co., Ltd.
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021-61312847 13636370518
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021-55068248
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shyysw007@163.com
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T&W GROUP
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021-61551611 13296011611
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+86 21-50676805
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contact@trustwe.com
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Hangzhou Sage Chemical Co., Ltd.
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+86057186818502 13588463833
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Pls mail us for more information!
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info@sagechem.com
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China
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Beijing Yisiyan Technology Research Center
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010-56645598 13366904824
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01062230033
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bjkaida@163.com
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Shanghai T&W Pharmaceutical Co., Ltd.
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+86 21 61551611
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+86 21 50676805
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130964-39-5, H-89 DIHYDROCHLORIDE HYDRATERelated Search:


  • H-89 DIHYDROCHLORIDE HYDRATE
  • H-89 hydrate dihydrochloride
  • H-89 dihydrochloride hydrate,N-[2-(p-Bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride
  • 5-IsoquinolinesulfonaMide, N-[2-[[3-(4-broMophenyl)-2-propenyl]aMino]ethyl]-, dihydrochloride
  • N-[2-[[3-(4-BroMophenyl)-2-propen-1-yl]aMino]ethyl]-5-isoquinolinesulfonaMide Hydrochloride
  • H 89 2HCl
  • Protein kinase inhibitor H-89 dihydrochloride
  • (E)-N-(2-(4-bromocinnamylamino)ethyl)isoquinoline-5-sulfonamide dihydrochloride
  • (E)-N-(2-((3-(4-bromophenyl)allyl)amino)ethyl)isoquinoline-5-sulfonamide dihydrochloride
  • H-89 Dihydrochloride Hydrate,N-[2-(P-Bromocinnamylamino)Et
  • N-[2-[[3-(4-Bromophenyl)-2-Propen-1-Yl]Amino]Ethyl]-5-Isoquinolinesulfonamide Hydrochloride (1:2)
  • PROTEIN KINASE INHIBITOR H-89 DIHYDROCHLORIDE; H89 DIHYDROCHLORIDE;H 89
  • H-89 dihydrochloride(Protein kinase inhibitor H-89 dihydrochloride)
  • PROTEIN KINASE INHIBITOR H-89 DIHYDROCHLORIDE;H89 DIHYDROCHLORIDE;H 89 DIHYDROCHLORIDE;H 89;H89
  • 5-Isoquinolinesulfonamide
  • H-89 DIHYDROCHLORIDE HYDRATE USP/EP/BP
  • 130964-39-5
  • Inhibitors
  • Akt
  • mTOR
  • PI3K
  • PI3K/Akt/mTOR
  • Protein Kinase Inhibitors and Activators