Basic information Description application Reference Safety Supplier Related
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MOPS

Basic information Description application Reference Safety Supplier Related

MOPS Basic information

Product Name:
MOPS
Synonyms:
  • MOPS
  • MOPS, CONCENTRATE SOLUTION
  • MORPHOLINOPROPANE SULFONIC ACID
  • TIMTEC-BB SBB009133
  • 4-MORPHOLINEPROPANESULFONIC ACID
  • 4-(MORPHOLINOPROPANE SULFONIC ACID)
  • 3-MORPHOLINOPROPANESULFONIC ACID
  • 3-MORPHOLINOPROPANESULPHONIC ACID
CAS:
1132-61-2
MF:
C7H15NO4S
MW:
209.26
EINECS:
214-478-5
Product Categories:
  • Imidazoles
  • Buffer
  • Miscellaneous Biochemicals
  • Organic acids
  • Biochemistry
  • Good's Buffers
Mol File:
1132-61-2.mol
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MOPS Chemical Properties

Melting point:
277-282 °C
Density 
1.3168 (rough estimate)
vapor pressure 
0Pa at 25℃
refractive index 
1.6370 (estimate)
Flash point:
116 °C
storage temp. 
room temp
solubility 
H2O: 1 M at 20 °C, clear
form 
Powder/Solid
color 
White
PH
2.5-4.0 (25℃, 1M in H2O)
pka
7.2(at 25℃)
PH Range
6.5 - 7.9
Odor
Odorless
Water Solubility 
1000 g/L (20 ºC)
λmax
λ: 260 nm Amax: 0.020
λ: 280 nm Amax: 0.015
Merck 
14,6265
BRN 
1106776
Stability:
Stable. Incompatible with strong bases, strong oxidizing agents.
InChIKey
DVLFYONBTKHTER-UHFFFAOYSA-N
LogP
-2.94 at 20℃
CAS DataBase Reference
1132-61-2(CAS DataBase Reference)
EPA Substance Registry System
4-Morpholinepropanesulfonic acid (1132-61-2)
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Safety Information

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36
WGK Germany 
1
RTECS 
QE9104530
TSCA 
Yes
HS Code 
29349990

MSDS

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MOPS Usage And Synthesis

Description

MOPS (3-morpholinopropanesulfonic acid) is a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES. Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.It is used as a synthetic buffering agent below pH 7.5.

application

MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis. Usage above 20 mM in mammalian cell culture work is not recommended. MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.

Reference

P. H. Quail, D. Marme, E. Schäfer, Particle-bound phytochrome from maize and pumpkin, Nature New Biology, 1973, vol. 245, pp. 189-191

Chemical Properties

White/clear crystalline powder

Uses

MOPS acts as a multi-purpose buffering agent used in various biological research.

Uses

Due to its inert nature, 3-morpholinopropanesulfonic acid (MOPS) is widely used as a buffer in many biochemical studies. MOPS has been used as a cell culture additive component in lentiviral particle production,  a buffering agent in microbial growth medium and nuclei extraction buffer, a component of Roswell Park Memorial Institute (RPMI) medium for diluting fungal inoculum, and a buffer in capillary-zone electrophoresis to test performance. It is also used for the dilution of proteins from algal samples.

Definition

ChEBI: 3-(N-morpholino)propanesulfonic acid is a Good's buffer substance, pKa = 7.2 at 20 ℃. It is a member of morpholines, a MOPS and an organosulfonic acid. It is a conjugate acid of a 3-(N-morpholino)propanesulfonate. It is a tautomer of a 3-(N-morpholiniumyl)propanesulfonate.

General Description

3-(N-Morpholino)propane sulfonic acid (MOPS) is an N-substituted amino sulfonic acid with a morpholinic ring. MOPS is capable of buffering within a pH range of 6.5-7.9. MOPS is widely used in biological and biochemical studies due to its inert properties. It does not interact with any metal ions in solutions and has significant metal-buffer stability especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions. MOPS buffer maintains the pH of the mammalian cell culture medium. MOPS functions to maintain pH in denaturing gel electrophoresis of RNA. MOPS can modify lipid interactions and influence the thickness and barrier properties of membranes. MOPS interacts with bovine serum albumin and stabilizes the protein. Hydrogen peroxide oxidizes MOPS slowly to N-oxide form.

Flammability and Explosibility

Not classified

Synthesis

Morpholine and 1,3-Propanesultone are added to absolute ethanol for reaction. The crude product synthesized is purified to obtain 3-Morpholinopropanesulfonic acid.

MOPSSupplier

Hubei New Desheng Materials Technology Co., Ltd. Gold
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027-87879183,027-87633098 15207106154
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