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4',5'-DIBROMOFLUORESCEIN

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4',5'-DIBROMOFLUORESCEIN Basic information

Product Name:
4',5'-DIBROMOFLUORESCEIN
Synonyms:
  • Dibromofluorescein (contains Mono-, Tri- and Tetra-)
  • 4′,5′-Dibromofluorescein,Solvent Red72
  • SOLVENT RED 72
  • 4',5'-Dibromof
  • 4',5'-DibroMofluorescein, ca. 95% dye content
  • 4',5'-dibromo-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
  • 4’,5’-dibromo-fluorescei
  • 4’,5’-dibromofluororescein
CAS:
596-03-2
MF:
C20H10Br2O5
MW:
490.1
EINECS:
209-876-0
Mol File:
596-03-2.mol
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4',5'-DIBROMOFLUORESCEIN Chemical Properties

Melting point:
270-273 °C(lit.)
Boiling point:
633.7±55.0 °C(Predicted)
Density 
1.6743 (rough estimate)
refractive index 
1.5010 (estimate)
storage temp. 
Sealed in dry,Room Temperature
Colour Index 
45370
pka
8.14±0.20(Predicted)
Merck 
14,3022
BRN 
57189
CAS DataBase Reference
596-03-2(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 4',5'-dibromo-3',6'-dihydroxy- (596-03-2)
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Safety Information

Hazard Codes 
Xi,T
Risk Statements 
36-25-36/37/38
Safety Statements 
22-24/25-45-36-26
RIDADR 
UN 2811 6.1/PG 3
WGK Germany 
3
RTECS 
LM5200000
TSCA 
Yes
HS Code 
32129000

MSDS

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4',5'-DIBROMOFLUORESCEIN Usage And Synthesis

Chemical Properties

orange powder

Uses

4'',5''-Dibromofluorescein is a stain with an absorption maximum of 450 nm. Dyes and metabolites, Environmental Testing

Preparation

Fluorescein (C.I. Acid Yellow 73, C.I. 45350) in the sodium hydroxide solution two bromination, and??made into a free acid form.

Safety Profile

Poison by ingestion and intravenous routes. An herbicide. When heated to decomposition it emits highly toxic fumes of NOx, CN-, and Br-. See also NITRILES.

Properties and Applications

brilliant yellow light yellow. Slightly soluble in water for orange, with weak yellow fluorescence, soluble in ethanol for orange, with green yellow fluorescence, soluble in acetone peachblossom, with yellow fluorescence, soluble in Tetrahydrofurfuryl alcohol. In concentrated sulfuric acid to red light yellow, dilution after yellow brown, with orange precipitation. Dye aqueous solution to join sodium hydroxide as red edge; In the glycerol and liquid paraffin in good dispersibility. Used in medicines and cosmetics coloring.

Purification Methods

Crystallise it from aqueous 30% EtOH. [Beilstein 19/6 V 462.]

4',5'-DIBROMOFLUORESCEINSupplier

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