NS 11394 Chemical Properties

Boiling point:

623.7±65.0 °C(Predicted)

Density 

1.17

storage temp. 

Store at -20°C

solubility 

≥35.3 mg/mL in DMSO with gentle warming; insoluble in H2O; ≥11.02 mg/mL in EtOH

form 

solid

pka

14.20±0.29(Predicted)

color 

White to yellow

NS 11394 Usage And Synthesis

Uses

NS11394 is an orally active and unique subtype-selective GABAA positive allosteric receptor (PAM), with a Ki of ~0.5 nM. NS11394 shows a selectivity profile in the order of GABAA-5 > α3 > α2 > α1-containing receptors. NS11394 has anxiolytic and anti-inflammatory properties[1][2][3].

Biological Activity

ns 11394 is a selective gaba(a) receptor-positive modulator.preclinical studies suggest that gabaa-1, 2, and 5-containing receptors can mediate the sedative/ motor-impairing, anxiolytic, and memory impairing effects, respectively.

in vitro

previous study showed that ns11394 possessed a functional selectivity profile at gaba(a) receptors of alpha(5) > alpha(3) > alpha(2) > alpha(1) based on oocyte electrophysiology with human gaba(a) receptors. moreover, compared with other subtype-selective ligands, ns11394 was unique in having superior efficacy for gaba(a)-alpha(3) receptors while maintaining low efficacy for gaba(a)-alpha(1) receptors [1].

in vivo

animal study showed that ns11394 had an excellent pk profile, which correlated with pharmacodynamic endpoints of cns receptor occupancy. in addition, it was showd that ns11394 was potent and highly effective in rodent anxiety models. the anxiolytic efficacy of ns11394 was most probably mediated via its high efficacy at gaba(a)-alpha(3) receptors, though the contribution of gaba(a)-alpha(2) receptors could not be excluded. moreover, when compared with benzodiazepines, ns11394 had a significantly reduced side effect profile in rat and mouse, even at full cns receptor occupancy. the authors attributed such benign side effect profile to very low efficacy of ns11394 at gaba(a)-alpha(1) receptors and a partial agonist profile of receptor subtypes. it was also found that ns11394 could impair memory in both rats and mice, which was possibly attributable to its efficacy at gaba(a)-alpha(5) receptors [1].

References

[1] mirza nr, et al. ns11394 [3'-[5-(1-hydroxy-1-methyl-ethyl)-benzoimidazol-1-yl]-biphenyl- 2-carbonitrile], a unique subtype-selective gabaa receptor positive allosteric modulator: in vitro actions, pharmacokinetic properties and in vivo anxiolytic efficacy. j pharmacol exp ther. 2008 dec;327(3):954-68.

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