HOECHST 33258 Chemical Properties

Melting point:

314 °C

Boiling point:

746.9±70.0 °C(Predicted)

Density 

1.355±0.06 g/cm3(Predicted)

storage temp. 

−20°C

solubility 

H₂O: 10 mg/mL

form 

powder

pka

9.23±0.15(Predicted)

color 

Light yellow to yellow

Stability:

Stable. Incompatible with strong oxidizing agents. Light sensitive.

EPA Substance Registry System

Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]- (23491-44-3)

Safety Information

Hazard Codes 

Xn

Risk Statements 

22-36/38

Safety Statements 

26-36

WGK Germany 

3

RTECS 

SM1140500

F 

3-8-10

MSDS

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HOECHST 33258 Usage And Synthesis

Chemical Properties

yellow solid

Uses

Hoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].

Definition

ChEBI: Pibenzimol is a bibenzimidazole and a N-methylpiperazine. It has a role as a fluorochrome and an anthelminthic drug.

References

[1] Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through inductionof apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300. DOI:10.1016/j.bmcl.2012.06.102
[2] Stoli? I, et al. Synthesis, DNA/RNA affinity and antitumour activity of new aromatic diamidines linked by 3,4-ethylenedioxythiophene. Eur J Med Chem. 2011 Feb;46(2):743-55. DOI:10.1016/j.ejmech.2010.12.010

HOECHST 33258(23491-44-3)Related Product Information

• Hoechst 33258
• HOECHST 33258
• Hoechst 33342
• 2'-(4-METHACRYLOXYPHENYL)-5-(4-METHYL-1-PIPER-AZINYL)-2,5'-BIBENZIMIDAZOLYL TRIHYDROCHLORIDE