MAG-INDO 1-AM Chemical Properties

Melting point:

>200℃

storage temp. 

2-8°C

solubility 

DMSO: soluble

form 

powder

color 

Pale yellow

λmax

354 nm

Biological Applications

Magnesium indicator; calcium indicator

Safety Information

WGK Germany 

3

F 

3-8-10

MAG-INDO 1-AM Usage And Synthesis

Description

Mag-indo-1 AM is a membrane-permeant fluorescent indicator for intracellular magnesium. It can be loaded into cells via incubation, and hydrolyzed to mag-indo-1 by cytosilic esterases.

Uses

Mag-indo-1/AM is a cell-permeable fluorescent indicator for Mg2+ and also for Ca2+ (Ex=340-390 nm, Em=410-490 nm). Mag-indo-1/AM can be used to determine intracellular Mg2+ and Ca2+ concentrations[1][2].

References

[1] Griffiths EJ. Calcium handling and cell contraction in rat cardiomyocytes depleted of intracellular magnesium. Cardiovasc Res. 2000 Jul;47(1):116-23. DOI:10.1016/s0008-6363(00)00061-4
[2] Morelle B, et al. Measurement of intracellular magnesium concentration in 3T3 fibroblasts with the fluorescent indicator Mag-indo-1. Anal Biochem. 1994 Apr;218(1):170-6. DOI:10.1006/abio.1994.1156

Excitation / Emission Maximum

537 nm/559 ±5 nm

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