BAPTA Chemical Properties

Melting point:

177-179 °C

Boiling point:

575.89°C (rough estimate)

Density 

1.4482 (rough estimate)

refractive index 

1.6500 (estimate)

RTECS 

MC0423000

storage temp. 

2-8°C

solubility 

Soluble in dimethyl sulfoxide

form 

Powder

pka

1.40±0.10(Predicted)

color 

White to off-white

Water Solubility 

Soluble in water and sodium bicarbonate.

λmax

209 nm, 254 nm, 203 nm, 274 nm

Merck 

14,957

BRN 

5192406

Stability:

Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.

Biological Applications

Calcium indicators; zinc indicators; treating acute cell death,cerebral infarction,myocardial infarction,hepatonecrosis,kidney ischemic necrosis,necrotizing pancreatitis,amyloidosis,atherosclerosis,diseases characteriz

InChIKey

FTEDXVNDVHYDQW-UHFFFAOYSA-N

CAS DataBase Reference

85233-19-8(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36-37/39

WGK Germany 

3

HS Code 

29225090

MSDS

• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

BAPTA Usage And Synthesis

Description

BAPTA free acid (85233-19-8) is a selective extracellular Ca2+ chelator. It is a widely used calcium sponge for chelating Ca2+?outside of intact cells.1 It’s a useful tool to probe the involvement of extracellular Ca2+ in cellular signaling.2,3 Non-cell-permeable.

Chemical Properties

White Solid

Uses

Highly selective calcium chelating reagent. Suitable for the spectrophotometric monitoring of Ca2+ levels.

Uses

BAPTA is a highly selective calcium chelating reagent. Lipophilic diesters of the chelating agent 1,2-Bis(2 aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) are used for inhibition of proteolytic activities of certain metalloproteinases, calpain and TACE.

Uses

As calcium chelator; 19F labelled forms for measurement of intracellular calcium.

Definition

ChEBI: A polyamino carboxylic acid in which bis(carboxymethyl)nitrilo groups are bonded to C-2 and C-2' of 1,1'-[ethane-1,2-diylbis(oxy)]dibenzene.

General Description

In nature 1,2-Bis(2-Aminophenoxy)ethane-N,N,N′,N′-tetra acetic acid (BAPTA) is a main metabolic product of BAPTA -AM (BAPTA-tetra(actoxymethyl ester), a neuro protective agent in cerebral ischemia, in rats.BAPTA is an intercellular Ca²⁺ chelator.

Biological Activity

Selective calcium chelator.

storage

Store at +4°C

References

1) Munoz et al. (2015), Different Stress-Induced Calcium Signatures Are Reported by Aequorin-Mediated Calcium Measurements in Living Cells of Aspergillus fumigatus; PLoS One, 10(9) e0138008 2) Wozniak et al. (2017), Extracellular Ca2+ is Required for Fertilization in the African Clawed Frog, Xenopus laevis; PLoS One, 12(1) e0170405 3) Didiasova et al. (2015), STIM1/ORAI1-mediated Ca2+ Influx Regulates Enolase-1 Exteriorization; J. Biol. Chem., 290 11983

BAPTA(85233-19-8)Related Product Information

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• 1-[2-(5-CARBOXYOXAZOL-2-YL)-6-AMINOBENZOFURAN-5-OXY]-2-(2'-AMINO-5'-METHYLPHENOXY)-ETHANE-N,N,N',N'-TETRAACETIC ACID, 5NA
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• NITR 5/AM
• INDO 1 PENTAPOTASSIUM SALT
• BAPTA, TETRACESIUM SALT
• BAPTA-TMFM