Fura 2-AM Chemical Properties

Melting point:

>250℃

Boiling point:

975.9±75.0 °C(Predicted)

Density 

1.400±0.06 g/cm3(Predicted)

storage temp. 

−20°C

solubility 

DMSO: soluble

form 

Powder

pka

1.89±0.50(Predicted)

color 

Yellow

Appearance

Solid Powder

Sensitive 

Light Sensitive

λmax

370nm

BRN 

8183748

Biological Applications

Calcium indicator; monitoring membrane potential; treating ischemia

InChIKey

VPSRLGDRGCKUTK-UHFFFAOYSA-N

SMILES

O1C(C(OCOC(C)=O)=O)=CN=C1C1=CC2=CC(OCCOC3=CC(C)=CC=C3N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C2O1

CAS DataBase Reference

108964-32-5(CAS DataBase Reference)

Safety Information

WGK Germany 

3

F 

8-10

MSDS

• Language:English Provider:SigmaAldrich

Fura 2-AM Usage And Synthesis

Description

Fura-2 AM is a cell-permeable fluorescent probe for detecting Ca2+ concentration.

Chemical Properties

yellow powder

Uses

High affinity, excitation-ratiometric fluorescent indicator for quantifying intracellular Ca2+ concentration

Uses

A calcium indicator; derivative of Fura-2 used to load the chelator into cells

Uses

Membrane-permeable analog of Fura 2 with high selectivity for Ca2+ binding. Fura 2-AM can be delivered into cells by microinjection or using Influx pinocytic cell-loading reagent. After crossing the membrane, the product is quickly metabolized by cytoplas

Biological Activity

Fluorescent Ca 2+ indicator. Selective for Ca 2+ over other divalent cations Mg 2+ , Zn 2+ , Fe 2+ and Mn 2+ . Used to determine intracellular Ca 2+ concentration.

storage

-20°C

Excitation / Emission Maximum

340-380 nm/510 nm

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