Basic information Chemical Properties Uses of Biology Uses Safety Supplier Related
ChemicalBook >  Product Catalog >  API >  Fluid, Electrolyte, and Acid-Base Balance >  Acid-base balance regulating drugs >  Tris Base

Tris Base

Basic information Chemical Properties Uses of Biology Uses Safety Supplier Related

Tris Base Basic information

Product Name:
Tris Base
Synonyms:
  • OmniPur TRIS -CAS 77-86-1 - Calbiochem
  • Tris Base, Molecular Biology Grade - CAS 77-86-1 - Calbiochem
  • Tris Base, ULTROL Grade - CAS 77-86-1 - Calbiochem
  • Tris Buffer, 1.0 M, pH 8.0, Molecular Biology Grade - CAS 77-86-1 - Calbiochem
  • Tris Buffer, 100 mM, pH 7.4, Molecular Biology Grade - CAS 77-86-1 - Calbiochem
  • Tris(Hydroxymethyl)aminomethane (Trometamol) high purity
  • 1-[ethyl(hydroxymethyl)amino]ethane-1,2-diol
  • TRIS,Tromethamine, Trometamol
CAS:
77-86-1
MF:
C4H11NO3
MW:
121.14
EINECS:
201-064-4
Product Categories:
  • tris
  • Pharmaceutical intermediates
  • API
  • ACS Grade Buffers
  • Amino Alcohols
  • Biological Buffers
  • Buffers A to Z
  • Building Blocks
  • Chemical Synthesis
  • Organic Building Blocks
  • Oxygen Compounds
  • Buffer
  • Biochemistry
  • Reagents for Electrophoresis
Mol File:
77-86-1.mol
More
Less

Tris Base Chemical Properties

Melting point:
167-172 °C(lit.)
Boiling point:
219-220 °C10 mm Hg(lit.)
Density 
1,353 g/cm3
vapor pressure 
0.0267 hPa (20 °C)
refractive index 
1.4170 (estimate)
Flash point:
219-220°C/10mm
storage temp. 
2-8°C
solubility 
H2O: 4 M at 20 °C, clear, colorless
form 
crystalline
color 
white
PH
10.5-12.0(4 m in water, 25 °C)
PH Range
7 - 9
pka
8.1(at 25℃)
Water Solubility 
550 g/L (25 ºC)
λmax
λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.08
Sensitive 
Hygroscopic
Merck 
14,9772
BRN 
741883
Stability:
Stable. Incompatible with bases, strong oxidizing agents. Protect from moisture.
InChIKey
LENZDBCJOHFCAS-UHFFFAOYSA-N
CAS DataBase Reference
77-86-1(CAS DataBase Reference)
NIST Chemistry Reference
1,3-Propanediol, 2-amino-2-(hydroxymethyl)-(77-86-1)
EPA Substance Registry System
Tris(hydroxymethyl)aminomethane (77-86-1)
More
Less

Safety Information

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36-37/39-24/25-23
WGK Germany 
2
RTECS 
TY2900000
3
TSCA 
Yes
HazardClass 
IRRITANT
HS Code 
29221980
Hazardous Substances Data
77-86-1(Hazardous Substances Data)
Toxicity
LD50 orally in Rabbit: > 5000 mg/kg LD50 dermal Rat > 5000 mg/kg

MSDS

More
Less

Tris Base Usage And Synthesis

Chemical Properties

White crystal or powder. Melting point 171-172℃, boiling point 219-220℃/1.3kPa, soluble in ethanol and water, slightly soluble in ethyl acetate, benzene, insoluble in ether, carbon tetrachloride,copper,aluminum corrosion effects,irritant.

Uses of Biology

Tris base, also known as THAM, is widely used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.

Uses

1, Tris buffer, only nucleic acids and proteins are widely used as solvents.
2, Tris is used for protein crystal growth under different pH conditions.
3, Tris buffer having a low ionic strength can be used nematode (C. elegans) is formed lamin (lamin) intermediate fibers.
4, Tris is a major component of protein electrophoresis buffer.
5, Tris for the preparation of a surfactant, a vulcanization accelerator, and some drug intermediates.
6, Tris titrated also used as standard.

Chemical Properties

White, crystalline solid. PH (0.1M aqueous solution) 10.6, corrosive to copper, brass, aluminum. Solubility in water 80 g/100 cc (20C). Combustible.

Originator

Trisaminol,Bellon,France,1964

Uses

tromethamine can help mask odor in a formulation. It is also used as a buffer, keeping product pH stable.

Uses

In the synthesis of surface-active agents, vulcanization accelerators, pharmaceuticals. As emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, leather dressings, textile specialties, polishes, cleaning Compounds, so-called soluble oils. Absorbent for acidic gases. Biological buffer.

Manufacturing Process

Nitromethane is reacted with formaldehyde to give tris(hydroxymethyl)nitromethane in an initial step. This intermediate may be reduced by catalytic hydrogenation (US Patent 2,174,242) or by electrolytic reduction (US Patent 2,485,982).

brand name

Tham (Hospira).

Therapeutic Function

Antacid

Biological Activity

Commonly used laboratory reagent

Purification Methods

TRIS can ordinarily be obtained in highly pure form suitable for use as an acidimetric standard. If only impure material is available, it should be crystallised from 20% EtOH, aqueous MeOH (m 171.1o) or isopropanol (m 172-173o). Dry it in a vacuum desiccator over P2O5 or CaCl2. Alternatively, it is dissolved in twice its weight of water at 55-60o, filtered, concentrated to half its volume and poured slowly, with stirring, into about twice its volume of EtOH. The crystals which separate on cooling to 3-4o are filtered off, washed with a little MeOH, air dried by suction, then finally ground and dried in a vacuum desiccator over P2O5. It has also been recrystallised from water, MeOH or aqueous MeOH, and vacuum dried at 80o for 2 days. [Beilstein 4 H 303, 4 III 857, 4 IV 1903.]

Tris Base Preparation Products And Raw materials

Raw materials

Tris BaseSupplier

Shangqiu tengfei biotechnology co., ltd Gold
Tel
;0370-63313-88
Email
1303279042@qq.com;1303279042@qq.com
Shanghai Jizhi Biochemical Technology Co. Ltd. Gold
Tel
400-900-4166
Email
3007522970@qq.com
Spectrum Chemical Manufacturing Corp. Gold
Tel
021-67601398-809
Email
marketing_china@spectrumchemical.com;
Shanghai Aladdin Bio-Chem Technology Co.,LTD Gold
Tel
021-20337333-801
Email
market@aladdin-e.com
Nanjing Search Biotech Co., Ltd Gold
Tel
025-85860978-0
Email
sales@searchbio.com.cn;