Tris Base Chemical Properties

Melting point:

167-172 °C(lit.)

Boiling point:

219-220 °C10 mm Hg(lit.)

Density 

1,353 g/cm³

vapor pressure 

0.0267 hPa (20 °C)

refractive index 

1.4170 (estimate)

Flash point:

219-220°C/10mm

storage temp. 

2-8°C

solubility 

H₂O: 4 M at 20 °C, clear, colorless

form 

crystalline

color 

white

PH

10.5-12.0(4 m in water, 25 °C)

PH Range

7 - 9

pka

8.1(at 25℃)

Water Solubility 

550 g/L (25 ºC)

λmax

λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.08

Sensitive 

Hygroscopic

Merck 

14,9772

BRN 

741883

Stability:

Stable. Incompatible with bases, strong oxidizing agents. Protect from moisture.

InChIKey

LENZDBCJOHFCAS-UHFFFAOYSA-N

CAS DataBase Reference

77-86-1(CAS DataBase Reference)

NIST Chemistry Reference

1,3-Propanediol, 2-amino-2-(hydroxymethyl)-(77-86-1)

EPA Substance Registry System

Tris(hydroxymethyl)aminomethane (77-86-1)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36-37/39-24/25-23

WGK Germany 

2

RTECS 

TY2900000

F 

3

TSCA 

Yes

HazardClass 

IRRITANT

HS Code 

29221980

Hazardous Substances Data

77-86-1(Hazardous Substances Data)

Toxicity

LD50 orally in Rabbit: > 5000 mg/kg LD50 dermal Rat > 5000 mg/kg

MSDS

• Language:English Provider:2-Amino-2-(hydroxymethyl)-1,3-propanediol
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

Tris Base Usage And Synthesis

Chemical Properties

White crystal or powder. Melting point 171-172℃, boiling point 219-220℃/1.3kPa, soluble in ethanol and water, slightly soluble in ethyl acetate, benzene, insoluble in ether, carbon tetrachloride,copper,aluminum corrosion effects,irritant.

Uses of Biology

Tris base, also known as THAM, is widely used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pK_a of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.

Uses

1, Tris buffer, only nucleic acids and proteins are widely used as solvents.
2, Tris is used for protein crystal growth under different pH conditions.
3, Tris buffer having a low ionic strength can be used nematode (C. elegans) is formed lamin (lamin) intermediate fibers.
4, Tris is a major component of protein electrophoresis buffer.
5, Tris for the preparation of a surfactant, a vulcanization accelerator, and some drug intermediates.
6, Tris titrated also used as standard.

Chemical Properties

White, crystalline solid. PH (0.1M aqueous solution) 10.6, corrosive to copper, brass, aluminum. Solubility in water 80 g/100 cc (20C). Combustible.

Originator

Trisaminol,Bellon,France,1964

Uses

tromethamine can help mask odor in a formulation. It is also used as a buffer, keeping product pH stable.

Uses

In the synthesis of surface-active agents, vulcanization accelerators, pharmaceuticals. As emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, leather dressings, textile specialties, polishes, cleaning Compounds, so-called soluble oils. Absorbent for acidic gases. Biological buffer.

Manufacturing Process

Nitromethane is reacted with formaldehyde to give tris(hydroxymethyl)nitromethane in an initial step. This intermediate may be reduced by catalytic hydrogenation (US Patent 2,174,242) or by electrolytic reduction (US Patent 2,485,982).

brand name

Tham (Hospira).

Therapeutic Function

Antacid

Biological Activity

Commonly used laboratory reagent

Purification Methods

TRIS can ordinarily be obtained in highly pure form suitable for use as an acidimetric standard. If only impure material is available, it should be crystallised from 20% EtOH, aqueous MeOH (m 171.1o) or isopropanol (m 172-173o). Dry it in a vacuum desiccator over P2O5 or CaCl2. Alternatively, it is dissolved in twice its weight of water at 55-60o, filtered, concentrated to half its volume and poured slowly, with stirring, into about twice its volume of EtOH. The crystals which separate on cooling to 3-4o are filtered off, washed with a little MeOH, air dried by suction, then finally ground and dried in a vacuum desiccator over P2O5. It has also been recrystallised from water, MeOH or aqueous MeOH, and vacuum dried at 80o for 2 days. [Beilstein 4 H 303, 4 III 857, 4 IV 1903.]

Tris Base(77-86-1)Related Product Information

• Trimethylolpropane
• TRIS hydrochloride
• N,N-Dimethyl-1,4-phenylenediamine
• ALTRENOGEST
• Lithium bis(trimethylsilyl)amide
• 4,4'-Methylenedianiline
• Tris(hydroxymethyl)nitromethane
• Tris Base
• TROMETAMOL HYDROCHLORIDE
• Betaine
• Glycine
• 2-METHYL-1,3-PROPANEDIOL
• 1,1,1-Tris(hydroxymethyl)ethane
• 1,3-Propanediol
• methylol cellulose
• 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol
• 2-Hydroxyethyl methacrylate
• 3-Amino-1,2-propanediol