TAPS Chemical Properties

Melting point:

230-235 °C (dec.)

Density 

1.4290 (rough estimate)

refractive index 

1.6370 (estimate)

Flash point:

110 °C

storage temp. 

Keep in dark place,Sealed in dry,Room Temperature

solubility 

H₂O: 1 M at 20 °C, clear, colorless

form 

Solid

pka

8.55; pKa (37°): 8.1; pKa2 (25°): 8.28

color 

White

Odor

Odorless

PH Range

7.7 - 9.1

Water Solubility 

Soluble

Merck 

13,9148

BRN 

2452420

Stability:

Stable. Incompatible with strong oxidizing agents

InChIKey

YNLCVAQJIKOXER-UHFFFAOYSA-N

CAS DataBase Reference

29915-38-6(CAS DataBase Reference)

EPA Substance Registry System

1-Propanesulfonic acid, 3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- (29915-38-6)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

22-24/25-36-26

WGK Germany 

3

TSCA 

Yes

HazardClass 

IRRITANT

HS Code 

29221980

MSDS

• Language:English Provider:3-[[2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]-1-propanesulfonic acid
• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

TAPS Usage And Synthesis

Description

TAPS is a zwitterionic buffering agent used in biochemistry and molecular biology. It is part of the Tris family of buffers and is useful for a pH range of 7.7 – 9.1. The members of TRIS family are tris(hydroxymethyl)aminomethane (TRIS), N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid (TES), N-[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid (TAPS), N-[tris(hydroxymethyl)methyl]-3-amino-2-hydroxypropanesulfonic acid (TAPSO), and N-tris(hydroxymethyl)methyl-4-aminobutanesulfonic acid (TABS) buffers[1]. The solubilities of TAPS in aqueous solution decrease with increasing concentration of the salts (salting-out effect)[2]. TAPS is the preferred culture media buffer used for dinoflagellate experiments, allowing for minimal pH change and maximal growth. It is also often used in capillary electrophoresis to analyze DNA and in planar chromatography to separate dyes. TAPS may form a complex with some common metals so stability constants and concentrations should be taken into account when choosing this buffer. TAPS is capable of inhibiting connexin channels in animal cells.

Chemical Properties

White/clear crystalline powder

Uses

A zwitterionic Good's Buffer

Definition

ChEBI: N-[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid is an organosulfonic acid.

Application

TAPS is a zwitterionic buffer used in biochemistry and molecular biology. It has a useful pH range of 7.7 - 9.1. TAPS has been utilized in capillary electrophoresis of DNA and DNA-dye complexes. Dyes can be separated by planar chromatography with electroosmotic flow containing 1 mM TAPS in the mobile phase. The stability constants of metal ions with TAPS have been investigated through the use of capillary electrophoresis. TAPS has also been shown to inhibit the activity of connexin channels. The activity of various carbonic anhydrases has been studied using TAPS physiological buffer, pKa = 8.4 at 20° C.

References

[1] Gupta B, et al. Stability Constants for the Equilibrium Models of Iron(III) with Several Biological Buffers in Aqueous Solutions. Journal of Solution Chemistry, 2013; 42: 2296–2309.
[2] Taha M, et al. New Insights into Buffer-Ionic Salt Interactions: Solubilities, Transfer Gibbs Energies, and Transfer Molar Volumes of TAPS and TAPSO from Water to Aqueous Electrolyte Solutions. Journal of Solution Chemistry, 2010; 39: 1665–1680.

TAPS(29915-38-6)Related Product Information

• Sulfamic acid
• BUFFER SOLUTION PH = 6,00 (20 °C) (POTASSIUM DIHYDROGEN PHOSPHATE/SODIUM HYDROXIDE)
• Tris(hydroxymethyl)nitromethane
• Molasses
• Nickel bis(sulphamidate)
• Formaldehyde sodium bisulfite
• Calcium dobesilate
• 4-Amino-benzenesulfonic acid monosodium salt
• Sodium isethionate
• Glycine
• Ethylparaben
• CHLOROPHOSPHONAZO III
• BIS-Tris
• Sulfanilic acid
• Ethyl acetate
• Tricine
• Tris(hydroxymethyl)aminomethane
• Tris(hydroxymethyl)aminomethane acetate salt