Tricine Chemical Properties

Melting point:

186-188 °C (dec.)

Boiling point:

311.69°C (rough estimate)

Density 

1.05 g/mL at 20 °C

refractive index 

1.4240 (estimate)

storage temp. 

Keep in dark place,Inert atmosphere,Room temperature

solubility 

H₂O: 4 M at 20 °C, clear, colorless

form 

crystalline

color 

clear colorless (40 % (w/w) in H₂O) solution

Odor

Odorless

PH

4.0-6.0 (1M in H2O)

PH Range

7.4 - 8.8

pka

8.1(at 25℃)

Water Solubility 

soluble

Merck 

14,9651

BRN 

1937804

Stability:

Stable.

CAS DataBase Reference

5704-04-1(CAS DataBase Reference)

EPA Substance Registry System

Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36-36/37/39

WGK Germany 

3

F 

3

TSCA 

Yes

HS Code 

29052900

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

Tricine Usage And Synthesis

Descriptioin

Tricine is a zwitterionic amino acid. It is a white crystalline powder. It was first prepared by Good to buffer chloroplast reaction.1 Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets^.1,2 Attributing to its higher negative charge than glycine, it can migrate faster. At the same time, its high ionic strength results in more ion movement and less protein movement. Therefore, it works as a buffer component for separation of low molecular weight peptides.³

Reference

1. S. R. Haider, B. L. Sharp, H. J. Reid, A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins, Journal of Separation Science, 2011, vol. 34, pp. 2463-2467
2. H. Schägger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, 1987, vol. 166, pp. 368-379

Chemical Properties

White/clear crystalline powder

Uses

Biological buffer.

Purification Methods

Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]

Tricine(5704-04-1)Related Product Information

• Kresoxim-methyl
• Sodium hydroxymethylglycinate
• ISOXADIFEN-ETHYL
• Tris Base
• Ethyl acetate
• Ethyl acrylate
• Methyl bromide
• CHLOROPHOSPHONAZO III
• Bensulfuron methyl
• Methylparaben
• TRIS-TRICINE,TRIS-TRICINE ANODE BUFFER
• Ethylparaben
• Ethanol
• Sarcosine
• Di(trimethylol propane)
• TRIS-TRICINE BUFFER
• GlycineMax
• 1,1,1-Tris(hydroxymethyl)ethane