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Tricine

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Tricine Basic information

Product Name:
Tricine
Synonyms:
  • LABOTEST-BB LT00454966
  • n-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-glycin
  • TRICINE
  • TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE
  • TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE
  • TRIS-HCL BUFFER
  • TRIClNE
  • E-Z 96 SIZE SELECT-IT (1 X 96)
CAS:
5704-04-1
MF:
C6H13NO5
MW:
179.17
EINECS:
227-193-6
Product Categories:
  • Biochemistry
  • Good's Buffers
  • Buffer
Mol File:
5704-04-1.mol
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Tricine Chemical Properties

Melting point:
186-188 °C (dec.)
Boiling point:
311.69°C (rough estimate)
Density 
1.05 g/mL at 20 °C
refractive index 
1.4240 (estimate)
storage temp. 
Store at RT.
solubility 
H2O: 4 M at 20 °C, clear, colorless
form 
crystalline
color 
clear colorless (40 % (w/w) in H2O) solution
Odor
Odorless
PH
4.0-6.0 (1M in H2O)
PH Range
7.4 - 8.8
pka
8.1(at 25℃)
Water Solubility 
soluble
Merck 
14,9651
BRN 
1937804
Stability:
Stable.
CAS DataBase Reference
5704-04-1(CAS DataBase Reference)
EPA Substance Registry System
Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1)
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Safety Information

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36-36/37/39
WGK Germany 
3
F 
3
TSCA 
Yes
HS Code 
29052900

MSDS

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Tricine Usage And Synthesis

Descriptioin

Tricine is a zwitterionic amino acid. It is a white crystalline powder. It was first prepared by Good to buffer chloroplast reaction.1 Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets.1,2 Attributing to its higher negative charge than glycine, it can migrate faster. At the same time, its high ionic strength results in more ion movement and less protein movement. Therefore, it works as a buffer component for separation of low molecular weight peptides.3

Reference

  1. S. R. Haider, B. L. Sharp, H. J. Reid, A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins, Journal of Separation Science, 2011, vol. 34, pp. 2463-2467
  2. H. Schägger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, 1987, vol. 166, pp. 368-379

Chemical Properties

White/clear crystalline powder

Uses

Biological buffer.

Purification Methods

Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]

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