ChemicalBook > Product Catalog > Biochemical Engineering > Amino Acids and Derivatives > Glycine derivative > Tricine
Tricine
Tricine Basic information
- Product Name:
- Tricine
- Synonyms:
-
- LABOTEST-BB LT00454966
- n-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-glycin
- TRICINE
- TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE
- TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE
- TRIS-HCL BUFFER
- TRIClNE
- E-Z 96 SIZE SELECT-IT (1 X 96)
- CAS:
- 5704-04-1
- MF:
- C6H13NO5
- MW:
- 179.17
- EINECS:
- 227-193-6
- Product Categories:
-
- Biochemistry
- Good's Buffers
- Buffer
- Mol File:
- 5704-04-1.mol
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Tricine Chemical Properties
- Melting point:
- 186-188 °C (dec.)
- Boiling point:
- 311.69°C (rough estimate)
- Density
- 1.05 g/mL at 20 °C
- refractive index
- 1.4240 (estimate)
- storage temp.
- Store at RT.
- solubility
- H2O: 4 M at 20 °C, clear, colorless
- form
- crystalline
- color
- clear colorless (40 % (w/w) in H2O) solution
- Odor
- Odorless
- PH
- 4.0-6.0 (1M in H2O)
- PH Range
- 7.4 - 8.8
- pka
- 8.1(at 25℃)
- Water Solubility
- soluble
- Merck
- 14,9651
- BRN
- 1937804
- Stability:
- Stable.
- CAS DataBase Reference
- 5704-04-1(CAS DataBase Reference)
- EPA Substance Registry System
- Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1)
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Safety Information
- Hazard Codes
- Xi
- Risk Statements
- 36/37/38
- Safety Statements
- 26-36-36/37/39
- WGK Germany
- 3
- F
- 3
- TSCA
- Yes
- HS Code
- 29052900
MSDS
- Language:English Provider:SigmaAldrich
- Language:English Provider:ACROS
- Language:English Provider:ALFA
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Tricine Usage And Synthesis
Descriptioin
Tricine is a zwitterionic amino acid. It is a white crystalline powder. It was first prepared by Good to buffer chloroplast reaction.1 Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets.1,2 Attributing to its higher negative charge than glycine, it can migrate faster. At the same time, its high ionic strength results in more ion movement and less protein movement. Therefore, it works as a buffer component for separation of low molecular weight peptides.3
Reference
- S. R. Haider, B. L. Sharp, H. J. Reid, A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins, Journal of Separation Science, 2011, vol. 34, pp. 2463-2467
- H. Schägger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, 1987, vol. 166, pp. 368-379
Chemical Properties
White/clear crystalline powder
Uses
Biological buffer.
Purification Methods
Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]
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Tricine(5704-04-1)Related Product Information
- Kresoxim-methyl
- Sodium hydroxymethylglycinate
- ISOXADIFEN-ETHYL
- Tris Base
- Ethyl acetate
- Ethyl acrylate
- Methyl bromide
- CHLOROPHOSPHONAZO III
- Bensulfuron methyl
- Methylparaben
- TRIS-TRICINE,TRIS-TRICINE ANODE BUFFER
- Ethylparaben
- Ethanol
- Sarcosine
- Di(trimethylol propane)
- TRIS-TRICINE BUFFER
- GlycineMax
- 1,1,1-Tris(hydroxymethyl)ethane