Tricine Chemical Properties

Melting point:

186-188 °C (dec.)

Boiling point:

311.69°C (rough estimate)

Density 

1.05 g/mL at 20 °C

vapor pressure 

0.002-0.004Pa at 20-25℃

refractive index 

1.4240 (estimate)

storage temp. 

Keep in dark place,Inert atmosphere,Room temperature

solubility 

H₂O: 4 M at 20 °C, clear, colorless

form 

crystalline

color 

clear colorless (40 % (w/w) in H₂O) solution

PH Range

7.4 - 8.8

Odor

Odorless

PH

4.0-6.0 (1M in H2O)

pka

8.1(at 25℃)

Water Solubility 

soluble

Merck 

14,9651

BRN 

1937804

Stability:

Stable.

LogP

-2.87 at 25℃

CAS DataBase Reference

5704-04-1(CAS DataBase Reference)

EPA Substance Registry System

Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36-36/37/39

WGK Germany 

3

F 

3

TSCA 

Yes

HS Code 

29052900

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

Tricine Usage And Synthesis

Descriptioin

Tricine is a zwitterionic amino acid. It is a white crystalline powder. It was first prepared by Good to buffer chloroplast reaction.1 Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets^.1,2 Attributing to its higher negative charge than glycine, it can migrate faster. At the same time, its high ionic strength results in more ion movement and less protein movement. Therefore, it works as a buffer component for separation of low molecular weight peptides.³

Reference

1. S. R. Haider, B. L. Sharp, H. J. Reid, A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins, Journal of Separation Science, 2011, vol. 34, pp. 2463-2467
2. H. Schägger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, 1987, vol. 166, pp. 368-379

Chemical Properties

White/clear crystalline powder

Uses

Good's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets. It is a zwitterionic amino acid commonly useful in the pH range of 7.4-8.8 and the pKa is 8.05 at 25°C. This buffer is also used as an eluent in asymmetrical flow field-flow fractionation (AFFFF).

Definition

ChEBI: N-tris(hydroxymethyl)methylglycine is a Good's buffer substance, pKa = 8.15 at 20 ℃. It is functionally related to a member of tris and a glycine. It is a tautomer of a N-tris(hydroxymethyl)methylammonioacetate.

General Description

Tricine and its salts are used as biological buffers to control the pH of body fluids in vitro and in vivo. It acts as a precursor for polymers, oxazolones (with carboxylic acids), and oxazolidines (with aldehydes). Trizma can serve as an alkalizing agent in treating acidosis of the blood.

Biological Activity

Electrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy.

Purification Methods

Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]

Tricine(5704-04-1)Related Product Information

• 1,1,1-Tris(hydroxymethyl)ethane
• Methyl acrylate
• Trimethylolpropane
• Kresoxim-methyl
• ISOXADIFEN-ETHYL
• Tris(hydroxymethyl)aminomethane
• Ethyl acetate
• Ethyl acrylate
• CHLOROPHOSPHONAZO III
• Bensulfuron methyl
• Methylparaben
• Ethylparaben
• Ethanol
• Sarcosine
• Di(trimethylol propane)
• Sodium hydroxymethylglycinate
• Methyl
• TRIS-TRICINE BUFFER