4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE Chemical Properties

Melting point:

208-210 °C

Boiling point:

507.24°C (rough estimate)

Density 

1.4163 (rough estimate)

refractive index 

1.5740 (estimate)

storage temp. 

-20°C

solubility 

DMF: 20 mg/mL, clear, colorless

form 

Crystalline solid

pka

12.87±0.70(Predicted)

color 

White to Off-White

λmax

360nm(Phosphate buffer sol.)(lit.)

BRN 

1693397

Stability:

Light Sensitive - Protect from Light

LogP

0.340 (est)

CAS DataBase Reference

37067-30-4(CAS DataBase Reference)

EPA Substance Registry System

2H-1-Benzopyran-2-one, 7-[[2-(acetylamino)-2-deoxy-.beta.-D-glucopyranosyl]oxy]-4-methyl- (37067-30-4)

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

10-21

HS Code 

29321900

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS

4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE Usage And Synthesis

Chemical Properties

4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE is White Crystalline Solid

Uses

4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE is used for the assay of b-Acetylglucosaminidase. A fluorogenic substrate used for the diagnosis of GM2 gangliosidoses in leukocytes. Genetic defects in human b-hexosaminidase isozy mes (Hex A & Hex B) result in various clinical and biochemical types of GM2 gangliosidosis. They include Tay Sachs disease, Sandhoff disease and other forms. Fluorescence: max. Abs. l = 325nm, max. Em. l = 378nm

Uses

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide has been used as a substrate for assaying

• β-N-acetylglucosaminidase (NAGase) from soil samples
• chitinase from Fusarium oxysporum using fluorimetric assay
• β-N-acetylhexosaminidases from Arabidopsis thaliana roots and leaves samples

General Description

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) contains chitin monomer, N-acetyl-D-glucosamine (NAG) and a 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF) moiety.

Biochem/physiol Actions

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) is a synthetic fluorogenic substrate for N-acetyl-β-D-glucosaminidase (NAGase). The enzyme functionality is assessed by its ability to hydrolyze the N-acetyl-D-glucosamine (NAG) residue in 4-MUF-NAG. 4-MUF-NAG is also a substrate for Chitinase and N-acetylhexosaminidase.

4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE(37067-30-4)Related Product Information

• Chloroacetone
• DEXTROSE
• FLUOROPENTACHLOROACETONE
• 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE
• 4-METHYLUMBELLIFERYL-N,N',N''-TRIACETYL-BETA-CHITOTRIOSIDE
• 4-Methylumbelliferyl2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside
• 4-Methylumbelliferyl3,4,6-tri-O-acetyl-2-deoxy-2-trifluoroacetamido-b-D-glucopyranoside
• 4-METHYLUMBELLIFERYL N-ACETYL-BETA-D-GLUCOSAMINIDE-6-SULFATE SODIUM SALT
• 4-Methylumbelliferyl 6-Sulfo-2-acetamido-2-deoxy-b-D-glucopyranoside, Potassium Salt
• 4-METHYLUMBELLIFERYL BETA-D-N,N'-DIACETYL-CHITOBIOSIDE
• 4-METHYLUMBELLIFERYL BETA-D-N,N',N',N''-TETRAACETYLCHITOTETRAOSIDE
• 4-Methylumbelliferyl 2-Acetamido-2-deoxy-3-O-(a-L-fucopyranosyl)-b-D-glucopyranoside
• 4-Methylumbelliferyl 2-Acetamido-2-deoxy-4-O-(a-L-fucopyranosyl)-b-D-glucopyranoside
• 4-METHYLUMBELLIFERYL LEWIS X TRISACCHARIDE
• 4-METHYLUMBELLIFERYL-N,N'-DIACETYL-BETA-D-CHITOBIOSIDE MONOHYDRATE
• 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE DIHYDRATE
• 250 MG 4-METHYLUMBELLIFERYL-N-ACETYL-BETA-D-GLUCOSAMINIDE ANALYT. GR.
• 4-METHYLUMBELLIFERYL 4-DEOXY-B-D-CHITOBIOSE PERACETATE