Basic information Safety Supplier Related

T3 RNA POLYMERASE

Basic information Safety Supplier Related

T3 RNA POLYMERASE Basic information

Product Name:
T3 RNA POLYMERASE
Synonyms:
  • T3 RNA POLYMERASE
MW:
0
Mol File:
Mol File
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T3 RNA POLYMERASE Chemical Properties

storage temp. 
-20°C
form 
solution
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T3 RNA POLYMERASE Usage And Synthesis

Uses

T3 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from a T3 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:

  • RNA or DNA blotting techniques
  • In situ hybridization
  • RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
  • Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.

General Description

With this enzyme, homogeneously labeled single-stranded RNA can be generated. Transcripts can be nonradioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P]- or [α-35S]-labeled nucleotides. T3 RNA polymerase is a DNA-dependent RNA polymerase that is highly specific for its promoter. Only DNA downstream from the T3 promoter will be transcribed.

T3 RNA POLYMERASESupplier

Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
Zhejiang Lianshuo Biotechnology Co., Ltd.
Tel
18616526224
Email
lianshuo@vip.126.com
Shanghai jingkang bioengineering co., ltd.
Tel
021-54721350 13524668266
Email
2881505714@qq.com
Shanghai Kanglang Biotechnology Co., Ltd.
Tel
021-61998208 18217752821
Email
sales@klbio.cn
Shanghai Sig Biotechnology Co., LTD
Tel
021-57810052 18930344717
Email
2089316240@qq.com