T3 RNA POLYMERASE

Product Name
T3 RNA POLYMERASE
Chemical Name
T3 RNA POLYMERASE
Synonyms
T3 RNA POLYMERASE
CBNumber
CB5360550
Formula Weight
0
MOL File
Mol file
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T3 RNA POLYMERASE Property

storage temp. 
-20°C
form 
solution
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
RPOLT3-RO
Product name
T3 RNA Polymerase
Purity
from Escherichia coli HB101
Packaging
1000UNITS
Price
$106
Updated
2022/05/15
Sigma-Aldrich
Product number
RPOLT3-RO
Product name
T3 RNA Polymerase
Purity
from Escherichia coli HB101
Packaging
5000UNITS
Price
$353
Updated
2022/05/15
American Custom Chemicals Corporation
Product number
ENZ0000962
Product name
T3 RNA POLYMERASE
Purity
95.00%
Packaging
5MG
Price
$504.68
Updated
2021/12/16
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T3 RNA POLYMERASE Chemical Properties,Usage,Production

Uses

T3 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from a T3 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:

  • RNA or DNA blotting techniques
  • In situ hybridization
  • RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
  • Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.

General Description

With this enzyme, homogeneously labeled single-stranded RNA can be generated. Transcripts can be nonradioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P]- or [α-35S]-labeled nucleotides. T3 RNA polymerase is a DNA-dependent RNA polymerase that is highly specific for its promoter. Only DNA downstream from the T3 promoter will be transcribed.

T3 RNA POLYMERASE Preparation Products And Raw materials

Raw materials

Preparation Products

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T3 RNA POLYMERASE Suppliers

Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
Country
China
ProdList
51456
Advantage
80
Zhejiang Lianshuo Biotechnology Co., Ltd.
Tel
18616526224
Email
lianshuo@vip.126.com
Country
China
ProdList
9607
Advantage
58
Shanghai jingkang bioengineering co., ltd.
Tel
021-54721350 13524668266
Fax
021-5
Email
2881505714@qq.com
Country
China
ProdList
7872
Advantage
58
Shanghai Kanglang Biotechnology Co., Ltd.
Tel
021-61998208 18217752821
Fax
021-37680378
Email
sales@klbio.cn
Country
China
ProdList
9618
Advantage
58
Shanghai Sig Biotechnology Co., LTD
Tel
021-57810052 18930344717
Fax
021-57810052
Email
2089316240@qq.com
Country
China
ProdList
9395
Advantage
58
Aleshan (Guangzhou) Biotechnology Co., Ltd
Tel
18636736628
Email
Araratbio@163.com
Country
China
ProdList
3342
Advantage
58
Nantong Feiyu Biological Technology CO.,LTD
Tel
0513-68819626; 15152877458
Fax
0513-68669626
Email
feiyubio@163.com
Country
China
ProdList
4819
Advantage
58
Shanghai Guchen Biotechnology Co., LTD
Tel
021-34675735 19147740836
Email
1986399151@qq.com
Country
China
ProdList
9844
Advantage
58
Shanghai Zheyan Biotech Co., Ltd.
Tel
021-18017610038 18017610038
Email
897493074@qq.com
Country
China
ProdList
5000
Advantage
58
Shanghai Ruili Spectrum Biotechnology Co., Ltd
Tel
021-64705760 18027310741
Email
1060496435@qq.com
Country
China
ProdList
4566
Advantage
58