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Deoxyribonuclease

Product Name
Deoxyribonuclease
CAS No.
9003-98-9
Chemical Name
Deoxyribonuclease
Synonyms
dnasw;DNASE;dnaase;DNaseA;dornase;dornava;DNASE I;DNAaseI;Dnase 1;80 
CBNumber
CB3692293
Molecular Formula
NULL
Formula Weight
0
MOL File
Mol file
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Deoxyribonuclease Property

Density 
100 g/mL at 20 °C
vapor pressure 
0.004Pa at 25℃
storage temp. 
-20°C
solubility 
Soluble in 0.15 M sodium chloride (5.0mg/L) clear.
form 
solution (clear, colorless)
color 
white
Water Solubility 
125g/L at 25℃
Merck 
13,2923
LogP
-1.3 at 20℃
EPA Substance Registry System
Nuclease, deoxyribo- (9003-98-9)
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Safety

Safety Statements 
23-24/25-22
WGK Germany 
3
RTECS 
RF0750000
10-21
TSCA 
Yes
HS Code 
35079090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P284Wear respiratory protection.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
D5025
Product name
Deoxyribonuclease I from bovine pancreas
Purity
TypeIV,lyophilizedpowder,≥2,000?Kunitzunits/mgprotein
Packaging
15000Units
Price
$116
Updated
2024/03/01
Sigma-Aldrich
Product number
D7291
Product name
Deoxyribonuclease I RNase-free solution from bovine pancreas
Packaging
0.5mg
Price
$260
Updated
2024/03/01
Sigma-Aldrich
Product number
D5025
Product name
Deoxyribonuclease I from bovine pancreas
Purity
TypeIV,lyophilizedpowder,≥2,000?Kunitzunits/mgprotein
Packaging
150000units
Price
$341
Updated
2024/03/01
Sigma-Aldrich
Product number
D5025
Product name
Deoxyribonuclease I from bovine pancreas
Purity
TypeIV,lyophilizedpowder,≥2,000?Kunitzunits/mgprotein
Packaging
375000units
Price
$744
Updated
2024/03/01
Sigma-Aldrich
Product number
D4527
Product name
Deoxyribonuclease I from bovine pancreas
Purity
TypeII,lyophilizedpowder,Protein≥80?%,≥2,000?units/mgprotein
Packaging
10000units
Price
$97.3
Updated
2024/03/01
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Deoxyribonuclease Chemical Properties,Usage,Production

Uses

Deoxyribonuclease I is used for the removal of DNA from protein samples. DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.1 It has also been used along with trypsin for the preparation of single cell suspension from rat testes. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis

Uses

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.

Uses

Amplification grade DNase I has been used for the digestion of DNA during isolation and purification of RNA. The purified RNA can be used for the synthesis of cDNA using RNA reverse transcriptase.

General Description

DNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas.

  • Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides.
  • Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D.
  • DNase I structure resembles the structure of to exonuclease III. It includes two central ? sheets. Each β sheet is composed of six β-strands. This complex of β sheets is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1]

Flammability and Explosibility

Not classified

Biochem/physiol Actions

DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.

Clinical Use

DNAse is a human endonuclease, normally present insaliva, urine, pancreatic secretions, and blood. The enzymecatalyzes the hydrolysis of extracellular DNA into oligonucleotides.Aerosolized recombinant human deoxyribonucleaseI (rhDNAse), dornase alfa, Pulmozyme, has beenformulated into an inhalation agent for the treatment of pulmonarydisease in patients with CF.Among the clinical manifestations of CF are obstructionof the airways by viscous, dehydrated mucus. Pulmonaryfunction is diminished, and microbes can become entrappedin the viscid matrix. A cycle of pulmonary obstruction andinfection leads to progressive lung destruction and eventualdeath before the age of 30 for most CF patients. The immunesystem responds by sending in neutrophils, and theseaccumulate and eventually degenerate, releasing largeamounts of DNA. The high levels of extracellular DNA releasedand the mucous glycoproteins are responsible for thedegenerating lung function. The DNA-rich secretions alsobind to aminoglycoside antibiotics typically used to treat theinfections. In vitro studies showed that the viscosity of thesecretions could be reduced by application of DNAse I.
Pulmozyme is approved for use in the treatment of CFpatients, in conjunction with standard therapies, to reducethe frequency of respiratory infections requiring parenteralantibiotics and to improve pulmonary function. The dose isdelivered at a level of 2.5 mg daily with a nebulizer.Pulmozyme is not a replacement for antibiotics, bronchodilators,and daily physical therapy.

Deoxyribonuclease Preparation Products And Raw materials

Raw materials

Preparation Products

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Deoxyribonuclease Suppliers

Beijing Baileisen Biotechnology Co., Ltd.
Tel
13581502185
Email
joe0415zhaxi@163.com
Country
China
ProdList
141
Advantage
58
Tunchem Pharm (Shanghai)Tech Co., Ltd.
Tel
13127540771
Email
sales@tunchem.com
Country
China
ProdList
41
Advantage
58
JiangSu Star Science Co.,Ltd
Tel
400-400-605-7200-7200 17312445705
Email
sales022@b2star.com
Country
China
ProdList
3761
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
021-61259108 18621169109
Fax
86-21-61259102
Email
market03@meryer.com
Country
China
ProdList
40228
Advantage
62
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24529
Advantage
81
Energy Chemical
Tel
021-021-58432009 400-005-6266
Fax
021-58436166
Email
sales8178@energy-chemical.com
Country
China
ProdList
44688
Advantage
61
JinYan Chemicals(ShangHai) Co.,Ltd.
Tel
13817811078
Fax
86-021-50426522,50426273
Email
sales@jingyan-chemical.com
Country
China
ProdList
9976
Advantage
60
Adamas Reagent, Ltd.
Tel
400-6009262 16621234537
Fax
021-64823266
Email
chenyj@titansci.com
Country
China
ProdList
14103
Advantage
59
Shanghai Hanhong Scientific Co.,Ltd.
Tel
021-54306202 13764082696
Email
info@hanhongsci.com
Country
China
ProdList
42958
Advantage
64
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View Lastest Price from Deoxyribonuclease manufacturers

Watson Biotechnology Co.,Ltd
Product
Deoxyribonuclease 9003-98-9
Price
US $0.00-0.00/kg
Min. Order
10kg
Purity
2183μ/mg
Supply Ability
100
Release date
2024-08-06
WUHAN FORTUNA CHEMICAL CO., LTD
Product
Deoxyribonuclease 9003-98-9
Price
US $0.00/g
Min. Order
1g
Purity
99%
Supply Ability
10kg/month
Release date
2021-07-20
Hebei Dangtong Import and export Co LTD
Product
Deoxyribonuclease 9003-98-9
Price
US $120.00-110.00/Grams
Min. Order
10Grams
Purity
99%
Supply Ability
100
Release date
2023-03-28

9003-98-9, DeoxyribonucleaseRelated Search:


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