T3 RNA POLYMERASE

Product Name
T3 RNA POLYMERASE
Chemical Name
T3 RNA POLYMERASE
Synonyms
T3 RNA POLYMERASE
CBNumber
CB5360550
Formula Weight
0
MOL File
Mol file
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T3 RNA POLYMERASE Property

storage temp. 
-20°C
form 
solution
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
RPOLT3-RO
Product name
T3 RNA Polymerase
Purity
from Escherichia coli HB101
Packaging
1000UNITS
Price
$106
Updated
2022/05/15
Sigma-Aldrich
Product number
RPOLT3-RO
Product name
T3 RNA Polymerase
Purity
from Escherichia coli HB101
Packaging
5000UNITS
Price
$353
Updated
2022/05/15
American Custom Chemicals Corporation
Product number
ENZ0000962
Product name
T3 RNA POLYMERASE
Purity
95.00%
Packaging
5MG
Price
$504.68
Updated
2021/12/16
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T3 RNA POLYMERASE Chemical Properties,Usage,Production

Uses

T3 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from a T3 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:

  • RNA or DNA blotting techniques
  • In situ hybridization
  • RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
  • Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.

General Description

With this enzyme, homogeneously labeled single-stranded RNA can be generated. Transcripts can be nonradioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P]- or [α-35S]-labeled nucleotides. T3 RNA polymerase is a DNA-dependent RNA polymerase that is highly specific for its promoter. Only DNA downstream from the T3 promoter will be transcribed.

T3 RNA POLYMERASE Preparation Products And Raw materials

Raw materials

Preparation Products

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T3 RNA POLYMERASE Suppliers

Wako Pure Chemical Industries, Ltd.
Tel
--
Fax
--
Email
labchem-tec@wako-chem.co.jp
Country
Japan
ProdList
6819
Advantage
80