T4 DNA LIGASE
- Product Name
- T4 DNA LIGASE
- CAS No.
- 9015-85-4
- Chemical Name
- T4 DNA LIGASE
- Synonyms
- abs60084;DNA LIGASE;EC 6.5.1.2;EC 6.5.1.1;IUB: 6.5.1.1;T4 DNA LIGASE;DNA LIGASE T4;RNA LIGASE, T4 PAHGE;Polynucleotide Ligase;DNA LIGASE, THERMOSTABLE
- CBNumber
- CB3279078
- Formula Weight
- 0
- MOL File
- Mol file
T4 DNA LIGASE Property
- storage temp.
- −20°C
- form
- buffered aqueous glycerol solution
- biological source
- Escherichia coli (NM 989)
- Specific Activity
- 4,000U/mL
Safety
- WGK Germany
- 3
- F
- 10
- TSCA
- Yes
- HS Code
- 3504009000
N-Bromosuccinimide Price
- Product number
- DNALIG-RO
- Product name
- T4 DNA Ligase
- Purity
- recombinant form of the enzyme from T4 phage
- Packaging
- 100units
- Price
- $95.9
- Updated
- 2025/07/31
- Product number
- DNALIG-RO
- Product name
- T4 DNA Ligase
- Purity
- recombinant form of the enzyme from T4 phage
- Packaging
- 500units
- Price
- $313
- Updated
- 2025/07/31
- Product number
- D2886
- Product name
- DNA Ligase from T4-infected Escherichia coli
- Purity
- buffered aqueous glycerol solution
- Packaging
- 100units
- Price
- $111
- Updated
- 2025/07/31
- Product number
- D2886
- Product name
- DNA Ligase from T4-infected Escherichia coli
- Purity
- buffered aqueous glycerol solution
- Packaging
- 500units
- Price
- $343
- Updated
- 2022/05/15
- Product number
- ENZ0000453
- Product name
- T4 DNA LIGASE
- Purity
- 95.00%
- Packaging
- 5MG
- Price
- $495.33
- Updated
- 2021/12/16
T4 DNA LIGASE Chemical Properties,Usage,Production
Uses
Forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. For ligation of cloning vector and restriction insert fragments
General Description
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.
Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.
Biological Activity
T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3μ hydroxyl and 5μ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks.
T4 DNA LIGASE Preparation Products And Raw materials
Raw materials
Preparation Products
T4 DNA LIGASE Suppliers
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