ChemicalBook > CAS DataBase List > T4 DNA LIGASE

T4 DNA LIGASE

Product Name: T4 DNA LIGASE
CAS No.: 9015-85-4
Chemical Name: T4 DNA LIGASE
Synonyms: abs60084;DNA LIGASE;EC 6.5.1.2;EC 6.5.1.1;IUB: 6.5.1.1;T4 DNA LIGASE;DNA LIGASE T4;RNA LIGASE, T4 PAHGE;Polynucleotide Ligase;DNA LIGASE, THERMOSTABLE
CBNumber: CB3279078
Formula Weight: 0
MOL File: Mol file

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T4 DNA LIGASE Property

storage temp.: −20°C
form: buffered aqueous glycerol solution
biological source: Escherichia coli (NM 989)
Specific Activity: 4,000U/mL

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Safety

WGK Germany: 3
F: 10
TSCA: Yes
HS Code: 3504009000

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: DNALIG-RO
Product name: T4 DNA Ligase
Purity: recombinant form of the enzyme from T4 phage
Packaging: 100units
Price: $95.9
Updated: 2025/07/31

Sigma-Aldrich

Product number: DNALIG-RO
Product name: T4 DNA Ligase
Purity: recombinant form of the enzyme from T4 phage
Packaging: 500units
Price: $313
Updated: 2025/07/31

Sigma-Aldrich

Product number: D2886
Product name: DNA Ligase from T4-infected Escherichia coli
Purity: buffered aqueous glycerol solution
Packaging: 100units
Price: $111
Updated: 2025/07/31

Sigma-Aldrich

Product number: D2886
Product name: DNA Ligase from T4-infected Escherichia coli
Purity: buffered aqueous glycerol solution
Packaging: 500units
Price: $343
Updated: 2022/05/15

American Custom Chemicals Corporation

Product number: ENZ0000453
Product name: T4 DNA LIGASE
Purity: 95.00%
Packaging: 5MG
Price: $495.33
Updated: 2021/12/16

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T4 DNA LIGASE Chemical Properties,Usage,Production

Uses

Forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. For ligation of cloning vector and restriction insert fragments

General Description

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.

Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.

Biological Activity

T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3μ hydroxyl and 5μ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks.

T4 DNA LIGASE Preparation Products And Raw materials

Raw materials

Preparation Products

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9015-85-4, T4 DNA LIGASERelated Search:

Benzyl isocyanide Ferric acetylacetonate Ethyl isocyanoacetate Cupric acetylacetonate N-BUTYLISOCYANIDE Tris(2,4-pentanedionato)chroMiuM(III) TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) 2,4-PENTANEDIONE, SILVER DERIVATIVE 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) SALCOMINE Aluminum acetylacetonate COBALT(II) ACETYLACETONATE METHYL ISOCYANOACETATE PHENYLSELENOL DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) Tosylmethyl isocyanide TERT-BUTYL ISOCYANIDE

T4 DNA LIGASE

RNA LIGASE, T4 PAHGE

DNA LIGASE

DNA LIGASE NAD-DEPENDENT

DNA LIGASE T4

DNA LIGASE, THERMOSTABLE

EC 6.5.1.1

EC 6.5.1.2

IUB: 6.5.1.1

T4 dna ligase from T4-infected E. coli

Synthetase, polydeoxyribonucleotide

dna ligase from t4-infected escherichia coli

polydeoxyribonucleotide synthase

Synthetase,polydeoxyribonucleotide (adenosine triphosphate)

Polynucleotide Ligase

RNA Ligase, T4 pahge, recombinant, Soln.

abs60084

DNA Ligase from T4-infected <i>Escherichia coli</i>

9015-85-4

BioChemical

Molecular Biology Enzymes

Molecular Biology

Modifying Enzymes

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