BROMOSULFALEIN Chemical Properties

solubility 

DMF: 5 mg/ml; DMSO: 5 mg/ml; Ethanol: slightly soluble; PBS (pH 7.2): 1 mg/ml

form 

Powder

Water Solubility 

Soluble in water.

Sensitive 

Moisture Sensitive

Merck 

14,8954

Stability:

Stable. Incompatible with strong oxidizing agents. May be air or moisture sensitive.

EPA Substance Registry System

Benzenesulfonic acid, 3,3'-(4,5,6,7-tetrabromo-3-oxo-1(3H)-isobenzofuranylidene)bis[6-hydroxy-, disodium salt (71-67-0)

Safety Information

Hazard Codes 

Xn

Risk Statements 

42/43

Safety Statements 

22-36/37

WGK Germany 

3

RTECS 

SM8395000

TSCA 

Yes

Hazardous Substances Data

71-67-0(Hazardous Substances Data)

MSDS

• Language:English Provider:SigmaAldrich

BROMOSULFALEIN Usage And Synthesis

Chemical Properties

white crystalline solid

Uses

Sulfobromophthalein sodium salt hydrate is a reagent for the quantitative determination of proteins, a dye for liver diagnostics in vitro.

Definition

ChEBI: An organic sodium salt that is the disodium salt of bromosulfophthalein.

Purification Methods

Purify it by TLC on silica Gel G (Merck 250a particle size) in two solvent systems (BuOH/AcOH/H2O 30:7.5:12.5 v/v, and BuOH/propionic acid/H2O 30:20:7.5 v/v). When the solvent reaches a height of ~10cm, the plate is removed, dried in air and developed with NH3 vapour giving blue-coloured spots. Also, the dye can be chromatographed on MN Silica Gel with t-BuOH/H2O/n-BuOH (32:10:5 v/v) as eluent and visualised with a dilute KOH (or NaOH if the Na salt is required) spray. The product corresponding to bromosulfalein is scraped off and eluted with H2O, filtered and evaporated to dryness in a vacuum. It was dissolved in H2O and filtered through Sephadex G-25 and evaporated to dryness. [UV and IR identification: Barbier & DeVeerdt J Pharm Sci 57 819 1968, NMR: Kato et al. Chem Pharm Bull Jpn 20 581 1972, McGuire Anal Biochem 83 75 1977, Beilstein 18/9 V 461.]

BROMOSULFALEIN(71-67-0)Related Product Information

• 5-Bromo-2-methylbenzoic acid
• 2-Bromo-6-methylbenzoic acid
• 1,4-DIBROMO-2-ETHYLBENZENE
• 2-Bromostyrene
• 4-Bromo-o-xylene
• Ethylenesulfonic acid
• 4-Bromo-2-methylbenzoic acid
• BROMOSULFALEIN,bromosulfalein sodium
• 2,5-Dibromobenzaldehyde
• BROMOSULFALEIN
• 3,4-Dibromobenzaldehyde
• 2-Bromobenzoic acid
• 2,5-Dibromotoluene
• 4-BROMO-2-ETHYLBENZALDEHYDE
• Sodium ethylenesulphonate
• 3-Bromo-2-methylbenzoic acid
• 2,3,5-TRIBROMOBENZOIC ACID
• 2,3-Dimethylbromobenzene