Bicinchoninic Acid Disodium Salt Chemical Properties

Melting point:

300℃

storage temp. 

Inert atmosphere,2-8°C

solubility 

0.5% sodium bicarbonate: soluble50mg/mL

form 

Powder/Chunks

color 

white to light yellow

Water Solubility 

Soluble in water.

Stability:

Hygrscopic

InChI

InChI=1S/C20H12N2O4.Na.H/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;/h1-10H,(H,23,24)(H,25,26);;

InChIKey

AUPXFICLXPLHBB-UHFFFAOYSA-L

SMILES

C(C1=CC(C2=NC3=CC=CC=C3C(C(=O)O)=C2)=NC2=CC=CC=C12)(=O)O.[NaH]

CAS DataBase Reference

979-88-4(CAS DataBase Reference)

EPA Substance Registry System

[2,2'-Biquinoline]-4,4'-dicarboxylic acid, disodium salt (979-88-4)

Safety Information

Hazard Codes 

Xn,Xi

Risk Statements 

36/37-42-36/38-36/37/38-20/21/22

Safety Statements 

22-26-36/37-45-36

WGK Germany 

3

TSCA 

Yes

HS Code 

29339900

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

Bicinchoninic Acid Disodium Salt Usage And Synthesis

Description

2,2’-Biquinoline-4,4’-dicarboxylic acid (BCA) is a colorimetric reagent for the detection of copper (Cu⁺). BCA and Cu⁺ form a stable 2:1 complex that has an absorbance maximum at 562 nm. It is commonly used as a reagent in the colorimetric BCA protein quantitation assay in which Cu²⁺ is converted to Cu⁺ as a function of protein concentration under alkaline conditions.

Chemical Properties

White to light yellow powder

Uses

For determination of Cu and protein assays.Bicinchoninic acid disodium salt is useful for the determination of copper. It is also used for the determination of protein concentration.

Uses

• Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.
• It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).
• It has been used for determining protein concentration.

Application

2,2'-Bicinchoninic Acid Disodium Salt is a photometric reagent for protein assay. It is used for determination of reducing sugar and protein via Cu(I). Also a specific reagent for Cu(I) with sensitivity similar to that of neocuproin.

Biochem/physiol Actions

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

Bicinchoninic Acid Disodium Salt (979-88-4)Related Product Information

• Ethyl 2-(Chlorosulfonyl)acetate
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• Folic acid
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• POLY(ETHYLENE GLYCOL) BIS(CARBOXYMETHYL) ETHER
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• 2,4-DIMETHYLQUINOLINE
• 2,2'-BIPYRIDINE-4-CARBALDEHYDE
• 2,2'-Bicinchoninic Acid