4-Nitrophenyl phosphate bis(cyclohexylammonium) salt Chemical Properties

Melting point:

199-200°C

storage temp. 

-20°C

form 

powder

Water Solubility 

water: 10mg/mL, clear, colorless to faintly yellow

BRN 

6553276

CAS DataBase Reference

52483-84-8(CAS DataBase Reference)

EPA Substance Registry System

Phosphoric acid, mono(4-nitrophenyl) ester, compd. with cyclohexanamine (1:2) (52483-84-8)

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

8-10-21

TSCA 

Yes

HS Code 

2940000080

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt Usage And Synthesis

Uses

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

Biological Activity

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product th at can be measured spectrophotometrically at 405 nm.

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt(52483-84-8)Related Product Information

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