Solvent Black 3 Chemical Properties

Melting point:

120-124 °C(lit.)

Boiling point:

552.68°C (rough estimate)

Density 

1.4899 (rough estimate)

refractive index 

1.4570 (estimate)

storage temp. 

Store at RT.

solubility 

Insoluble in water; soluble in ethanol, acetone, benzene, toluene, xylene, ethylene glycol

form 

Powder/Solid

Colour Index 

26150

pka

2.94±0.40(Predicted)

color 

Very dark brown to black

Water Solubility 

Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol, acetone, benzene, toluene and hydrocarbon. Insoluble in water.

λmax

598 nm, 415 nm

Merck 

13,8970

BRN 

723248

Stability:

Light Sensitive

Biological Applications

Diagnosis of acute myeloid leukemia (AML); detecting neuron-specific nuclear protein NeuN; drug screening

CAS DataBase Reference

4197-25-5(CAS DataBase Reference)

EPA Substance Registry System

C.I. Solvent Black 3 (4197-25-5)

Safety Information

Hazard Codes 

F,Xi

Risk Statements 

11-36/37/38

Safety Statements 

24/25-16-37/39-26

WGK Germany 

3

RTECS 

SD4431500

TSCA 

Yes

HazardClass 

IRRITANT

HS Code 

32041900

Toxicity

LD50 ivn-mus: 63 mg/kg CSLNX* NX#04918

MSDS

• Language:English Provider:C.I. 26150
• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich

Solvent Black 3 Usage And Synthesis

Chemical Properties

It is a very dark brown to black fine crystalline powder. It is soluble in ethanol, toluene, acetone, and other solvents. In concentrated sulfuric acid, it is violet-black; when diluted, it is dark greenish-blue and produces a blue to black precipitate. The ethanol solution of the dye is bluish-black when concentrated hydrochloric acid is added and dark blue when concentrated sodium hydroxide solution is added.

Uses

Sudan Black is a basic black dye that is used in staining triglycerides and lipids off some lipoproteins. Biological stain. Dyes and metabolites, Environmental Testing.

Definition

ChEBI: Sudan black B is a member of the class of perimidines that is 2,2-dimethyl-2,3-dihydro-1H-perimidine carrying a [4-(phenyldiazenyl)naphthalen-1-yl]diazenyl substituent at position 6. A fat-soluble dye predominantly used for demonstrating triglycerides in frozen sections and for staining of protein bound lipids in paraffin sections. It has a role as a histological dye. It is a member of azobenzenes, a bis(azo) compound and a member of perimidines.

Preparation

aniline diazotization, and Naphthalen-1-amine coupling, and its product to diazotization, and 2,2-Dimethyl-2,3-dihydro-1H-perimidine?coupling.

Safety Profile

A poison by intravenous route. When heated to decomposition it emits toxic vapors of NOx.

Staining Procedures

1 % Sudan black B is dissolved in 70% ethanol, the tissue is stained 1-1.5 h, subsequently washed in 70% ethanol (3 x 15 s), and mounted in 75% glycerol before microscopy. This staining method reveals positive results for lipids.

Properties and Applications

Blue-black. Soluble in ethanol, acetone and toluene. In concentrated sulfuric acid for purple light black, dilution after deep green light blue, blue to black precipitation. Dye ethanol solution to join concentrated hydrochloric acid for blue black; Join concentrated sodium hydroxide solution for skipper.

Solvent Black 3(4197-25-5)Related Product Information

• 2,6-DIMETHYLNAPHTHALENE
• Dimethyl fumarate
• Dimethyl phthalate
• Dimethyl sulfate
• Chlorodimethylphenylsilane
• Sudan I
• Solvent Red 23
• Dimethyl carbonate
• ETHANE
• Dimethyl sulfone
• Dimethyl sulfoxide
• Dimethicone
• Dacthal
• Xylene
• DBU
• Dimethyl ether
• N,N-Dimethylacetamide
• Poly(dimethylsiloxane)