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Solvent Black 3

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Solvent Black 3 Basic information

Product Name:
Solvent Black 3
Synonyms:
  • LABOTEST-BB LT00451850
  • 1H-Perimidine, 2,3-dihydro-2,2-dimethyl-6-((4-(phenylazo)-1-naphthyl)azo)-
  • Fast Black HB
  • Solvent Black 2
  • Sudan black b, high purity biological stain
  • SUDAN BLACK B (CERT)
  • 2,3-Dihydro-2,2-dimethyl-6-[[4-(phenylazo)naphthalen-1-yl]azo]-1H-perimidine
  • 2,2-Dimethyl-6-{[4-(phenyldiazenyl)-1-naphthyl]-diazenyl}-2,3-dihydro-1H-perimidine
CAS:
4197-25-5
MF:
C29H24N6
MW:
456.54
EINECS:
224-087-1
Product Categories:
  • Azo
  • stain
  • Organics
  • Solvent Dyestuff
Mol File:
4197-25-5.mol
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Solvent Black 3 Chemical Properties

Melting point:
120-124 °C(lit.)
Boiling point:
552.68°C (rough estimate)
Density 
1.4899 (rough estimate)
refractive index 
1.4570 (estimate)
storage temp. 
Store at RT.
solubility 
Insoluble in water; soluble in ethanol, acetone, benzene, toluene, xylene, ethylene glycol
form 
Powder/Solid
Colour Index 
26150
pka
2.94±0.40(Predicted)
color 
Very dark brown to black
Water Solubility 
Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol, acetone, benzene, toluene and hydrocarbon. Insoluble in water.
λmax
598 nm, 415 nm
Merck 
13,8970
BRN 
723248
Stability:
Light Sensitive
Biological Applications
Diagnosis of acute myeloid leukemia (AML); detecting neuron-specific nuclear protein NeuN; drug screening
CAS DataBase Reference
4197-25-5(CAS DataBase Reference)
EPA Substance Registry System
C.I. Solvent Black 3 (4197-25-5)
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Safety Information

Hazard Codes 
F,Xi
Risk Statements 
11-36/37/38
Safety Statements 
24/25-16-37/39-26
WGK Germany 
3
RTECS 
SD4431500
TSCA 
Yes
HazardClass 
IRRITANT
HS Code 
32041900
Toxicity
LD50 ivn-mus: 63 mg/kg CSLNX* NX#04918

MSDS

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Solvent Black 3 Usage And Synthesis

Chemical Properties

It is a very dark brown to black fine crystalline powder. It is soluble in ethanol, toluene, acetone, and other solvents. In concentrated sulfuric acid, it is violet-black; when diluted, it is dark greenish-blue and produces a blue to black precipitate. The ethanol solution of the dye is bluish-black when concentrated hydrochloric acid is added and dark blue when concentrated sodium hydroxide solution is added.

Uses

Sudan Black is a basic black dye that is used in staining triglycerides and lipids off some lipoproteins. Biological stain. Dyes and metabolites, Environmental Testing.

Definition

ChEBI: Sudan black B is a member of the class of perimidines that is 2,2-dimethyl-2,3-dihydro-1H-perimidine carrying a [4-(phenyldiazenyl)naphthalen-1-yl]diazenyl substituent at position 6. A fat-soluble dye predominantly used for demonstrating triglycerides in frozen sections and for staining of protein bound lipids in paraffin sections. It has a role as a histological dye. It is a member of azobenzenes, a bis(azo) compound and a member of perimidines.

Preparation

aniline diazotization, and Naphthalen-1-amine coupling, and its product to diazotization, and 2,2-Dimethyl-2,3-dihydro-1H-perimidine?coupling.

Safety Profile

A poison by intravenous route. When heated to decomposition it emits toxic vapors of NOx.

Staining Procedures

1 % Sudan black B is dissolved in 70% ethanol, the tissue is stained 1-1.5 h, subsequently washed in 70% ethanol (3 x 15 s), and mounted in 75% glycerol before microscopy. This staining method reveals positive results for lipids.

Properties and Applications

Blue-black. Soluble in ethanol, acetone and toluene. In concentrated sulfuric acid for purple light black, dilution after deep green light blue, blue to black precipitation. Dye ethanol solution to join concentrated hydrochloric acid for blue black; Join concentrated sodium hydroxide solution for skipper.

Standard Light Fastness Heat-resistant(℃) water Sodium Carbonate(5%) Hydrochloric acid(5%)
Melting point Stable
ISO General 126 100 Insoluble No change No change

Solvent Black 3 Preparation Products And Raw materials

Raw materials

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