Direct Blue 14 Chemical Properties

Melting point:

>300 °C (lit.)

Density 

1.007 g/mL at 20 °C

storage temp. 

dry at room temperature

solubility 

H2O: soluble10mg/mL

form 

Powder

Colour Index 

23850

color 

Dark greenish-brown

PH

9.8 (10g/l, H2O, 20℃)

Water Solubility 

10 g/L (25 ºC)

Merck 

14,9792

BRN 

4360496

Stability:

Stable. Incompatible with strong oxidizing agents.

CAS DataBase Reference

72-57-1(CAS DataBase Reference)

IARC

2B (Vol. 8, Sup 7) 1987

EPA Substance Registry System

Trypan blue (72-57-1)

Safety Information

Hazard Codes 

T,Xi

Risk Statements 

45-41-37/38-36/37/38-22

Safety Statements 

53-45-36/37/39-36-26

RIDADR 

2811

WGK Germany 

3

RTECS 

QJ6475000

TSCA 

Yes

HS Code 

32041400

Hazardous Substances Data

72-57-1(Hazardous Substances Data)

Toxicity

LD100 i.v. in rats: 300 mg/kg (Anderson)

MSDS

• Language:English Provider:Direct Blue 14
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

Direct Blue 14 Usage And Synthesis

Chemical Properties

blueish grey powder

Chemical Properties

Trypan Blue is a dark Blue crystalline solid or powder.

Uses

Biological stain.

Preparation

3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-2,7-disulfonic acid(2 Moore) coupled.

Definition

ChEBI: An organosulfonate salt that is the tetrasodium salt of 3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonic acid).

General Description

Bluish-gray to dark blue powder.

Air & Water Reactions

Azo dyes can be explosive when suspended in air at specific concentrations. Slightly soluble in water.

Reactivity Profile

Direct Blue 14 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 14 is incompatible with strong oxidizing agents and strong acids.

Health Hazard

ACUTE/CHRONIC HAZARDS: Direct Blue 14 may cause irritation and may be absorbed through the skin. It is a positive animal carcinogen. When heated to decomposition Direct Blue 14 emits very toxic fumes of nitrogen oxides, sulfur oxides, carbon monoxide and carbon dioxide.

Fire Hazard

Flash point data for Direct Blue 14 are not available; however, Direct Blue 14 is probably combustible.

Safety Profile

Suspected carcinogen with experimental carcinogenic, neoplastigenic, and tumorigenic data. Poison by intraperitoneal, intravenous, and subcutaneous routes. Experimental teratogenic and reproductive effects. Mutation data reported. When heated to decomposition it emits very toxic fumes of NOx NazO, and SOx

Potential Exposure

Used in dyeing textiles; leather and paper; as a biological stain.

Shipping

UN2811 Toxic solids, organic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required. UN3143 dyes, solid, toxic, n.o.s. or dye intermediates, solid, toxic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required.

Properties and Applications

blue. Blue gray powder. Soluble in water for blue, slightly soluble in soluble fiber element, insoluble in other organic solvents. The strong sulfuric acid in dark green light for blue, diluted into a red light blue; In the light of nitric acid for palm gray solution. The dye solution to join a red sulfuric acid 10% change; Add thick sodium hydroxide solution for red light purple.

Standard	Acid Resistance	Alkali Resistance	Light Fastness	Soaping		Water
				Fading	Stain	Fading	Stain
ISO	4-5	4	1-2	2		2
AATCC	5	2	1-2	2		1

Incompatibilities

Dust may form explosive mixture with air. Trypan Blue is incompatible with strong oxidizing agents. Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides. Azo compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acylhalides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combinations can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Trypan Blue is sensitive to prolonged exposure to heat.

Waste Disposal

Consult with environmental regulatory agencies for guidance on acceptable disposal practices. Generators of waste containing this contaminant (≥100 kg/mo) must conform with EPA regulations governing storage, transportation, treatment, and waste disposal.

Direct Blue 14(72-57-1)Related Product Information

• ACID RED 8
• 1,2,4-Benzenetriol
• Disodium 3-aminonaphthalene-2,7-disulphonate
• 1-Aminonaphthalene-6-sulfonic acid
• 6-Amino-2-naphthalenesulfonic acid
• 4-AMINO-3-METHYLBIPHENYL
• 8-AMINO-1-NAPHTHOL-3,6-DISULFONIC ACID DISODIUM SALT
• CI NO 22610
• Benzidine
• 5-amino-4-hydroxynaphthalene-2-sulphonic acid
• 2-Chloro-5-hydroxypyridine
• 3,3'-Dimethylbiphenyl
• amino-R acid
• 2,4-Dimethyl aniline
• Naphthalene-2-sulfonic acid
• 1-Naphthol-3,6-disulfonic acid
• 1-NAPHTHOL-3-SULFONIC ACID SODIUM SALT
• Direct Blue 14