Trypan Blue Chemical Properties

Melting point:

>300 °C (lit.)

Density 

1.007 g/mL at 20 °C

storage temp. 

dry at room temperature

solubility 

H2O: soluble10mg/mL

form 

Powder

Colour Index 

23850

color 

Dark greenish-brown

PH

9.8 (10g/l, H2O, 20℃)

Water Solubility 

10 g/L (25 ºC)

Merck 

14,9792

BRN 

4360496

Stability:

Stable. Incompatible with strong oxidizing agents.

CAS DataBase Reference

72-57-1(CAS DataBase Reference)

IARC

2B (Vol. 8, Sup 7) 1987

EPA Substance Registry System

Trypan blue (72-57-1)

Safety Information

Hazard Codes 

T,Xi

Risk Statements 

45-41-37/38-36/37/38-22

Safety Statements 

53-45-36/37/39-36-26

RIDADR 

2811

WGK Germany 

3

RTECS 

QJ6475000

TSCA 

Yes

HS Code 

32041400

Hazardous Substances Data

72-57-1(Hazardous Substances Data)

Toxicity

LD100 i.v. in rats: 300 mg/kg (Anderson)

MSDS

• Language:English Provider:Direct Blue 14
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

Trypan Blue Usage And Synthesis

Chemical Properties

Trypan Blue is a dark Blue crystalline solid or powder that dissolves in water to form a blue solution. It's slightly soluble in soluble fiber yarn and doesn't dissolve in other organic solvents. When mixed with concentrated sulfuric acid, it turns dark greenish-blue, and when diluted, it becomes reddish-blue. With concentrated nitric acid, it creates a brownish-gray solution. In a 10% sulfuric acid solution, its aqueous solution turns red, while with sodium hydroxide, it becomes reddish-purple.

Uses

Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. Dyes and metabolites.

Uses

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Definition

ChEBI: Trypan blue is an organosulfonate salt that is the tetrasodium salt of 3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonic acid). It has a role as a histological dye, a fluorochrome and a carcinogenic agent. It is an organosulfonate salt and an organic sodium salt. It contains a trypan blue(4-).

Preparation

3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-2,7-disulfonic acid(2 Moore) coupled.

General Description

Bluish-gray to dark blue powder.

Air & Water Reactions

Azo dyes can be explosive when suspended in air at specific concentrations. Slightly soluble in water.

Reactivity Profile

Direct Blue 14 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 14 is incompatible with strong oxidizing agents and strong acids.

Health Hazard

ACUTE/CHRONIC HAZARDS: Direct Blue 14 may cause irritation and may be absorbed through the skin. It is a positive animal carcinogen. When heated to decomposition Direct Blue 14 emits very toxic fumes of nitrogen oxides, sulfur oxides, carbon monoxide and carbon dioxide.

Fire Hazard

Flash point data for Direct Blue 14 are not available; however, Direct Blue 14 is probably combustible.

Biochem/physiol Actions

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

Safety Profile

Suspected carcinogen with experimental carcinogenic, neoplastigenic, and tumorigenic data. Poison by intraperitoneal, intravenous, and subcutaneous routes. Experimental teratogenic and reproductive effects. Mutation data reported. When heated to decomposition it emits very toxic fumes of NOx NazO, and SOx

Potential Exposure

Used in dyeing textiles; leather and paper; as a biological stain.

Shipping

UN2811 Toxic solids, organic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required. UN3143 dyes, solid, toxic, n.o.s. or dye intermediates, solid, toxic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required.

Staining Procedures

A 0.01-0.2% aqueous solution is used for root staining. The method is often used for investigation of mycorrhizas. Suitable for whole mounts as well as sections. Also used as a stain for vitality, as living cells generally remain unstained.

Properties and Applications

blue. Blue gray powder. Soluble in water for blue, slightly soluble in soluble fiber element, insoluble in other organic solvents. The strong sulfuric acid in dark green light for blue, diluted into a red light blue; In the light of nitric acid for palm gray solution. The dye solution to join a red sulfuric acid 10% change; Add thick sodium hydroxide solution for red light purple.

Standard	Acid Resistance	Alkali Resistance	Light Fastness	Soaping		Water
				Fading	Stain	Fading	Stain
ISO	4-5	4	1-2	2		2
AATCC	5	2	1-2	2		1

Incompatibilities

Dust may form explosive mixture with air. Trypan Blue is incompatible with strong oxidizing agents. Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides. Azo compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acylhalides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combinations can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Trypan Blue is sensitive to prolonged exposure to heat.

Waste Disposal

Consult with environmental regulatory agencies for guidance on acceptable disposal practices. Generators of waste containing this contaminant (≥100 kg/mo) must conform with EPA regulations governing storage, transportation, treatment, and waste disposal.

Trypan Blue(72-57-1)Related Product Information

• CI NO 22610
• DIRECT SCARLET B
• CHLORAZOL BLACK BH
• CHICAGO SKY BLUE 6B
• DIRECT FAST BROWN M
• DIRECT RED 80
• CHLORAZOL VIOLET N
• DIRECT PURE YELLOW 5G
• Direct Yellow 12
• SIRIUS YELLOW GC
• DIRECT FAST RED 3B
• DIRECT FAST YELLOW 5GL
• DIRECT FAST BROWN BX
• Benzidine
• 2-Chloro-5-hydroxypyridine
• 3,3'-Dimethylbiphenyl
• 2,4-Dimethyl aniline
• Naphthalene-2-sulfonic acid