Basic information Safety Supplier Related

2′,7′-Dichlorodihydrofluorescein Diacetate

Basic information Safety Supplier Related

2′,7′-Dichlorodihydrofluorescein Diacetate Basic information

Product Name:
2′,7′-Dichlorodihydrofluorescein Diacetate
Synonyms:
  • 187263
  • 2,7-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem
  • 2,7-Dichlorodihydrofluorescein diacetate (2,7-Dichlorofluorescin diacetate
  • 2',7'-DICHLOROFLUORESCIN DIACETATE, FOR FLUORESCENCE
  • 2',7'-Dichlorodihydrofluoresceinediacetate
  • Dichlorofluorescin diacetate
  • 2'',7''-Dichlorofluorescein diac
  • 2-(2,7-Dichloro-3,6-diacetoxy-9H-xanthen-9-yl)benzoic acid
CAS:
4091-99-0
MF:
C24H16Cl2O7
MW:
487.29
EINECS:
685-843-8
Mol File:
4091-99-0.mol
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2′,7′-Dichlorodihydrofluorescein Diacetate Chemical Properties

Melting point:
209-210 °C(lit.)
Boiling point:
593.5±50.0 °C(Predicted)
Density 
1.463±0.06 g/cm3(Predicted)
storage temp. 
-20°C
solubility 
DMF: soluble
form 
Crystalline solid
pka
3.79±0.36(Predicted)
BRN 
4610273
Stability:
Light Sensitive
EPA Substance Registry System
2',7'-Dichlorohydrofluorescein diacetate (4091-99-0)
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Safety Information

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
22-24/25-26
WGK Germany 
3
10-21
HS Code 
29329990
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2′,7′-Dichlorodihydrofluorescein Diacetate Usage And Synthesis

Description

DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M−1cm−1).

Chemical Properties

white to yellow-beige or pale pink powder

Uses

Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide.

Uses

2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2’7’dichlorofluorescein upon oxidation.

Uses

2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.

General Description

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (?NO) and for the determination of the degree of overall oxidative stress.

Biochem/physiol Actions

Cell permeable: yes

storage

Store at -20°C

2′,7′-Dichlorodihydrofluorescein DiacetateSupplier

Beijing Jin Ming Biotechnology Co., Ltd. Gold
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010-60605840 18892239720
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Tianjin Kailiqi Biotechnology Co., Ltd. Gold
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15076683720
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Tianjin Being Technology Co., Ltd. Gold
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18622939839
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Guangzhou Younan Technology Co., Ltd Gold
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020-020-82000279 18988968278
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Dalian Meilun Biotech Co., Ltd. Gold
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0411-62910999 13889544652
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sales@meilune.com