BCA PROTEIN ASSAY KIT Chemical Properties

storage temp. 

10-30°C

Water Solubility 

Fully miscible in water.

Safety Information

Storage Class

10 - Combustible liquids

Hazard Classifications

Aquatic Acute 1
Aquatic Chronic 1
Eye Dam. 1

BCA PROTEIN ASSAY KIT Usage And Synthesis

Uses

BCA Protein Assay Kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5?to 30??g/mL in tube assays and 1?to 20??g/mL in 96- or 384-well plate assays. It is used for studying protein: protein interactions, measuring column fractions after affinity chromatography, estimating percent recovery of membrane proteins from cell extracts and high-throughput screening of fusion proteins.

Description

The BCA Protein Assay Kit can be used to measure the protein concentration of lysates or homogenates, in microplate format, prepared with the following buffers: Cell Lysis Buffer (10X)

Background

Bicinchoninic Acid is capable of forming an intense purple complex with cuprous ion, Cu1+, in an alkaline environment. Cu1h+ is produced from the reaction of protein with alkaline Cu2+. The resulting reaction and color produced is the basis for a common protein quantification method capable of measuring protein concentration over a wide range. Increasing protein concentrations produce proportionally deeper colors. The BCA protein assay demonstrates higher tolerances towards common interfering substances, such as nonionic detergents and buffer salts, than the Lowry technique.

References

[1] Smith, P.K. et al. (1985) Anal Biochem 150, 76-85.

BCA PROTEIN ASSAY KIT()Related Product Information

• Acid Blue 83
• Bradford Protein Assay Kit
• Lowry Protein Assay Kit
• Acid Blue 90
• BCA protein concentration determination kit
• BRADFORD Protein Concentration Assay Kit (Detergent Compatible)
• BCA Protein Concentration Kit (Enhanced)
• Protein concentration assay kit
• Protein Standard (5MG/ML BSA)
• Folin-Phenol Reagent
• Modified BCA Protein Assay Kit
• Non-Interference Protein Assay Kit
• Modified Bradford Protein Assay Kit
• Protein dotMETRIC(TM) Kit
• Stable Lowry Protein Assay Kit
• Bradford reagent, solution Bradford
• Micro BCA Protein Assay Kit
• reducing agent