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L-Isoleucine

Product Name
L-Isoleucine
CAS No.
73-32-5
Chemical Name
L-Isoleucine
Synonyms
ILE;H-ILE-OH;L-Ile;L-lsoleucine;(2S,3S)-2-AMINO-3-METHYLPENTANOIC ACID;L-Ile-OH;(2S)-2-AMINO-3-METHYLPENTANOIC ACID;H-lle-OH;Isoleucin;Lsoleucine
CBNumber
CB2678131
Molecular Formula
C6H13NO2
Formula Weight
131.17
MOL File
73-32-5.mol
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L-Isoleucine Property

Melting point:
288 °C (dec.) (lit.)
alpha 
41 º (c=4, 6N HCl)
Boiling point:
225.8±23.0 °C(Predicted)
Density 
1.2930 (estimate)
FEMA 
4675 | L-ISOLEUCINE
refractive index 
40.5 ° (C=4, 6mol/L HCl)
Flash point:
168-170°C
storage temp. 
Store below +30°C.
solubility 
1 M HCl: 50 mg/mL
form 
powder
pka
2.32, 9.76(at 25℃)
color 
White to Off-white
PH
5.5-6.5 (40g/l, H2O, 20℃)
Odor
odorless
optical activity
[α]20/D +41.0±1°, c = 5% in 6 M HCl
Water Solubility 
41.2 g/L (50 ºC)
λmax
λ: 260 nm Amax: 0.07
λ: 280 nm Amax: 0.05
Merck 
14,5179
Sublimation 
168-170 ºC
JECFA Number
2118
BRN 
1721792
InChIKey
AGPKZVBTJJNPAG-WHFBIAKZSA-N
LogP
0.80
CAS DataBase Reference
73-32-5(CAS DataBase Reference)
NIST Chemistry Reference
Isoleucine(73-32-5)
EPA Substance Registry System
L-Isoleucine (73-32-5)
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Safety

Hazard Codes 
Xn
Risk Statements 
40
Safety Statements 
24/25-36-22
WGK Germany 
3
RTECS 
NR4705000
TSCA 
Yes
HS Code 
29224995
Hazardous Substances Data
73-32-5(Hazardous Substances Data)
Toxicity
LD50 intraperitoneal in rat: 6822mg/kg
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
8.16008
Product name
(S)-(+)-Isoleucine
Purity
for synthesis
Packaging
10g
Price
$38.3
Updated
2024/03/01
Sigma-Aldrich
Product number
8.16008
Product name
(S)-(+)-Isoleucine
Purity
for synthesis
Packaging
25g
Price
$55.6
Updated
2024/03/01
Sigma-Aldrich
Product number
56241
Product name
L-Isoleucine
Purity
certified reference material, TraceCERT
Packaging
100mg
Price
$134
Updated
2024/03/01
Sigma-Aldrich
Product number
1349502
Product name
L-Isoleucine
Purity
United States Pharmacopeia (USP) Reference Standard
Packaging
200mg
Price
$466
Updated
2024/03/01
TCI Chemical
Product number
I0181
Product name
L-Isoleucine
Purity
>98.0%(T)
Packaging
25g
Price
$45
Updated
2024/03/01
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L-Isoleucine Chemical Properties,Usage,Production

Description

Crystalline leaflets or a white, crystalline powder having a bitter taste. It is soluble in 25 parts of water, slightly soluble in hot alcohol, and soluble in diluted mineral acids and in alkaline solutions. It sublimes at between 168°C and 170°C, and melts with decomposition at about 284°C. The pH of a 1 in 100 solution is between 5.5 and 7.0.
Absolute: The absolute is prepared by alcoholic extraction of the concrete in approximately 55 to 60% yields. The absolute consists of a semisolid, olive-green mass with a characteristic ambergris odor.
Resinoid and Resin Absolute: The resinoid consists of the hydro carbon extract of the crude resin. The resin absolute consists of the alcoholic extract of the crude labdanum resin. Since this prod uct is a semisolid, nonpourable mass, a high-boiling, odorless solvent may be added prior to evaporation of the alcohol to prepare a pourable liquid.

Chemical Properties

White crystalline powder

Uses

L-Isoleucine acts as an essential amino acid in human. It is used as a flavoring agent and nutritional supplement for foods. It is also used as an antipsychotic. It is employed as a component of proteins. Further, it stimulates immune function and promotes secretion of several hormones. In addition to this, it is used for hemoglobin formation and regulating blood sugar and energy levels.

Uses

L-Isoleucine may be used as a pharmaceutical reference standard for the quantification of the analyte in pharmaceutical formulations using high-performance liquid chromatography technique.

Uses

L-Isoleucine, also known as Isoleucine, is an amino acid that is an isomer of leucine. It is important in hemoglobin synthesis and regulation of blood sugar and energy levels.
L-Isoleucine is one of the essential amino acids that cannot be made by the body and is known for its ability to help endurance and assist in the repair and rebuilding of muscle. This amino acid is important to body builders as it helps boost energy and helps the body recover from training.
L-Isoleucine is also classified as a branched-chain amino acid (BCAA). There are three branched-chain amino acids in the body, with the others being L-Valine, and L-Leucine, and all of them help promote muscle recovery after exercise. Isoleucine is actually broken down for energy within the muscle tissue.

Production Methods

An advantageous fermentation method for production of L-isoleucine is the use of chemically synthesized substrates that only require a few steps to be converted to L-isoleucine. Among these the natural precursors 2-ketobutyrate or D,L-2-hydroxybutyrate have been used for the production with Corynebacterium glutamicum. A leucine requiring mutant of Corynebacterium glutamicum, with increased D-lactate utilization and consuming D,L-2-hydroxybutyrate, accumulates 13.4 g/L L-isoleucine. However, exploitation of this process is hampered by formation of byproducts. Sugar based L-isoleucine processes have been developed with strains of Corynebacterium glutamicum Serratia marcescens, and Escherichia coli. The mutant Escherichia coli H- 8285, being resistant to thiaisoleucine, arginine hydroxamate, and D,L-ethionine accumulates 26 g/L L-isoleucine in 45 h in a fed-batch process. Introduction of resistance to 6-dimethylaminopurine in strain H-8285 resulted in a mutant Escherichia coli H-8461 that increased L-isoleucine accumulation to 30.2 g/L. The biosynthesis of L-isoleucine has been investigated in detail on the level of involved genes, thus recombinant strains are being constructed with high productivity and selectivity. An appropriate balance of homoserine dehydrogenase and threonine dehydratase activities in the construct Corynebacterium glutamicum DR17/pECM3::ilvA (V323A) with feedback-resistant aspartate kinase create a specific productivity of 0.052 g L-isoleucine per gram dry biomass per hour. The recombinant strain Escherichia coli AJ13100 produces L-isoleucine from glucose with high selectivity in 30% yield.

Definition

ChEBI: The L-enantiomer of isoleucine.

Biochem/physiol Actions

L-Isoleucine is an isomer of L-leucine and is an essential amino acid. It is synthesized from threonine and is a branched-chain hydrophobic amino acid.

Side effects

L-Isoleucine is generally considered a safe ingredient. The side effects may: Intake of Isoleucine can lead to nausea. Large doses can result in a stomach upset and indigestion.

Source

L-Isoleucine is an essential amino acid, which means that humans cannot synthesize it, so it must be ingested. It appears as a white crystalline powder. Foods that have high amounts of L-Isoleucineinclude eggs, soy protein, seaweed, turkey, chicken, lamb, cheese, and fish. This product is often used as a food additive and nutrient in dietary supplements.

Purification Methods

Crystallise L-isoleucine from H2O by addition of 4 volumes of EtOH or from aqueous MeOH. It sublimes at 170-181o/0.3mm with 99.7% recovery, unracemised [Gross & Gradsky J Am Chem Soc 77 1678 1955]. [Greenstein & Winitz The Chemistry of the Amino Acids J. Wiley, Vol 1 p 183-191, Vol 3 pp 2043-2073 1961, Huffman & Ingersoll J Am Chem Soc 73 3366 1951, Beilstein 4 IV 2775.]

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L-Isoleucine Suppliers

Hebei chuangzhiyuan biotechnology co., LTD
Tel
13012111162
Email
2792365312@qq.com
Country
China
ProdList
805
Advantage
58
Wuxi Bikang Bioengineering Co., Ltd
Tel
0510-80245888 400-0510-518
Fax
0510-80245999
Email
1258079079@qq.com
Country
China
ProdList
46
Advantage
58
Zhengzhou Yuhe Food Additive Co. LTD
Tel
0371-56060979 13838386843
Fax
0371-56060979
Email
2922109373@qq.com
Country
China
ProdList
507
Advantage
58
Shandong Pingju Biotechnology Co., Ltd
Tel
18613662662 15650537670
Email
2708672464@qq.com
Country
China
ProdList
1139
Advantage
58
Jiangsu Duoyang Bioengineering Technology Co., Ltd
Tel
15161148838
Email
2234255617@qq.com
Country
China
ProdList
921
Advantage
58
Shandong Aicai Biotechnology Co. , Ltd.
Tel
135-37016375 13573016375
Email
3358761976@qq.com
Country
China
ProdList
511
Advantage
58
Xian Lavia Biotechnology Co., LTD
Tel
18629620162
Email
1600587581@qq.com
Country
China
ProdList
428
Advantage
58
Hebei Jijie Biotechnology Co., Ltd
Tel
15803101794 15803101794
Email
740313571@qq.com
Country
China
ProdList
607
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
PharmaBlock Sciences (Nanjing),Inc.
Tel
025-86918202 4000255188
Fax
86-025-86918232
Email
sales@pharmablock.com
Country
China
ProdList
4986
Advantage
55
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View Lastest Price from L-Isoleucine manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
L-Isoleucine 73-32-5
Price
US $0.00/Kg/Drum
Min. Order
1KG
Purity
98.5%-101.5%
Supply Ability
10 Tons
Release date
2021-06-30
Sichuan HongRi Pharma-Tech Co.,Ltd
Product
L-Isoleucine 73-32-5
Price
US $0.00-0.00/kg
Min. Order
1kg
Purity
98% 99%
Supply Ability
1T+
Release date
2023-09-18
Hebei Weibang Biotechnology Co., Ltd
Product
L-Isoleucine 73-32-5
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
500000kg
Release date
2024-10-30

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