Coumarin 120 Property

Melting point:

223-226 °C (lit.)

Boiling point:

306.47°C (rough estimate)

Density 

1.1999 (rough estimate)

refractive index 

1.5060 (estimate)

storage temp. 

2-8°C

solubility 

Soluble in acetone (10 mg/ml) dimethyl sulfoxide (10 mg/ml) and dimethylformamide.

form 

Crystalline Powder

pka

1.89 ± 0.20, most basic, temperature: 25 °C

color 

Beige to brown

Sensitive 

Light Sensitive

λmax

351 nm (MeOH); 342 nm (Buffer pH 7)

BRN 

142231

Major Application

For authentification; dyelasers; color display devices; inks;monitoring underlying corrosion on coated aluminum;photoinitiating systems; recording materials;semiconductor devices; dye-sensitized solar cells(DSCs); mesoporous silica fibers; SiO2 sonogelhybrid materials; textiles; waveguides;whitening agents,

Biological Applications

Identifyingbacteria; measuring urinary kallikrein levels;monitoring platinum(iv) reduction and platinum(ii)reactions in cancer cells; as a substrate for measuringadipinylamidases activity, aromatic amino aciddecarboxylases activity, histone deacetylasesactivity, hydrolases/esterases activity,β-lactamases activity, microbial nitroreductasesactivity, penicillin G acylases activity,proteases/proteinases/peptidases activity,glutamyltransferases activity, tryptophanasesactivity; as an effective antitubercular agent; use astemperature sensor,

InChIKey

GLNDAGDHSLMOKX-UHFFFAOYSA-N

CAS DataBase Reference

26093-31-2(CAS DataBase Reference)

NIST Chemistry Reference

Coumarin, 7-amino-4-methyl-(26093-31-2)

EPA Substance Registry System

2H-1-Benzopyran-2-one, 7-amino-4-methyl- (26093-31-2)

Safety

Hazard Codes 

Xi,Xn

Risk Statements 

36/37/38-20/21/22

Safety Statements 

26-36-37/39

WGK Germany 

3

Hazard Note 

Irritant

TSCA 

Yes

HS Code 

32049000

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

Coumarin 120 Chemical Properties,Usage,Production

Chemical Properties

Coumarin 120 is Light yellow beige powder

Uses

Coumarin 120 is widely used fluorophore, well-known for preparing fluorogenic substrates for cystine aminopeptidase and other hydrolases. Used as reference compound in enzyme assays.

Uses

suitable for determining polygalacturonase activity

Uses

λ max 354 nm in EthanolSuitable as laser dye and used as a fluorescent labeling reagent for trace determination of enzymes.

Definition

ChEBI: Coumarin 120 is a member of 7-aminocoumarins. It has a role as a fluorochrome.

General Description

7-Amino-4-methylcoumarin (coumarin120) is an aminocoumarin laser dye that can emit laser in the range of 370-760 nm. It has three intermolecular hydrogen bonded structures with C-H···O and N-H····O.

Biochem/physiol Actions

7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors.

storage

+4°C

Purification Methods

Dissolve it in 5% HCl, filter and basify with 2M ammonia. The precipitate is dried in a vacuum and recrystallised from dilute EtOH. It yields a blue solution and is light sensitive. [Sigler Synthesis 614 1980, Kanaoka et al. Chem Pharm Bull Jpn 30 1485 1982, Beilstein 18/11 V 445.]