WST-8

Description Cell Counting Kit-8 (CCK-8)

Product Name: WST-8
CAS No.: 193149-74-5
Chemical Name: WST-8
Synonyms: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2h-tetrazolium sodium salt;WST-8;GLT008;CS-2725;fophenyL;WST8;WST 8;WST-8 sodium;)-5-(2,4-disuL;ium Sodium SaL;)-3-(4-nitrophenyL
CBNumber: CB4547211
Molecular Formula: C20H15N6NaO11S2
Formula Weight: 602.48
MOL File: 193149-74-5.mol

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WST-8 Property

storage temp.: under inert gas (nitrogen or Argon) at 2-8°C
solubility: DMSO: 1 mg/ml; PBS (pH 7.2): 5 mg/ml
form: A crystalline solid

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H314Causes severe skin burns and eye damage

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P330+P331IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P310Immediately call a POISON CENTER or doctor/physician.

P321Specific treatment (see … on this label).

P363Wash contaminated clothing before reuse.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Cayman Chemical

Product number: 18721
Product name: WST-8
Purity: ≥98%
Packaging: 10mg
Price: $33
Updated: 2024/03/01

Cayman Chemical

Product number: 18721
Product name: WST-8
Purity: ≥98%
Packaging: 50mg
Price: $112
Updated: 2024/03/01

Cayman Chemical

Product number: 18721
Product name: WST-8
Purity: ≥98%
Packaging: 100mg
Price: $160
Updated: 2024/03/01

Cayman Chemical

Product number: 18721
Product name: WST-8
Purity: ≥98%
Packaging: 500mg
Price: $443
Updated: 2024/03/01

TRC

Product number: W500038
Product name: WST-8
Packaging: 100mg
Price: $250
Updated: 2021/12/16

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WST-8 Chemical Properties,Usage,Production

Description

WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt), a highly stable and water-soluble WST, is utilized incell Counting Kit-8 (CCK-8).It is more sensitive than WST-1 particularly at neutralpH. Because of the electron mediator, 1-methoxy PMSin this kit is highly stable, and CCK-8 is stable for at least six months at the room temperature and for one year at 0-5°C (asshown in Figure 1).

Advantages: WST-8 is not cell permeable, which results in low cytotoxicity. Therefore,after the assay, it is possible to continue further experiments using the same cells.Furthermore, it produces the water-soluble formazan upon cellular reduction，which would provide an additional advantage to the method by allowing a simpler assay procedure and not required an extra step to dissolve the formazan.
Disadvantages: An important consideration is that reduction of assay substrates isimpacted by changes in intracellular metabolic activity that has no direct effect on over-all cell viability.

Cell Counting Kit-8 (CCK-8)

Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing the highly water-soluble tetrazolium salt WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2Htetrazolium,monosodium salt] (Patent No. WO97/38985) produces a water-soluble formazan dye upon reduction in the presence of an electron carrier. Cell Counting Kit-8 is a onebottle solution; no premixing of components is required. Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give a yellowcolored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.

Uses

A highly sensitive tetrazolium reagent that produces a water-soluble formazan (orange color that absorbs at 460 nm) in NADH. It is newer generation formazan-based dyes which release the converted prod uct into the medium in a soluble form allowing for a non-destructive determination of viability enabling the cells to be subject to further investigations.

Uses

WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH.

Biotechnological Applications

WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nM. Useful as a cell viability indicator in cell proliferation assays.

WST-8 Preparation Products And Raw materials

Raw materials

Preparation Products

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WST-8 Suppliers

Canada 1 China 221 India 4 Italy 1 United Kingdom 2 United States 12 Global 241

Nanjing enjing Biotechnology Co., Ltd

Tel: 025-86616616 13305189663
Fax: 025-86990398
Email: 2426321791@qq.com
Country: China
ProdList: 1
Advantage: 58

Jilin Chinese Academy of Sciences - Yanshen Technology Co., Ltd

Tel: 0431-80514535 19917294565
Email: et@chemextension.com
Country: China
ProdList: 4111
Advantage: 55

Shanghai Better-In Pharmaceutical Technology Co.,Ltd.

Tel: 021-38921049 13564871374
Fax: +86-21-38921049
Email: sales@better-in.com
Country: China
ProdList: 44
Advantage: 55

Shanghai shuji biotechnology co., ltd.

Tel: 021-021-62200788 15000569291
Email: zhanlingrui@163.com
Country: China
ProdList: 490
Advantage: 58

Shandong Kangsaide Biological Technology Co. LTD

Tel: 13475906826 18615616891
Email: zhangdong.0602@163.com
Country: China
ProdList: 81
Advantage: 58

Shandong Zhishang Chemical Co. LTD

Tel: 15689709505 15508639737
Email: 2138533552@qq.com
Country: China
ProdList: 47
Advantage: 58

Hubei jian chu biological technology co., LTD

Tel: 027-027-65528353 13554206703
Email: 3279834739@qq.com
Country: China
ProdList: 1075
Advantage: 58

Zhangshu Chenxi Biotechnology Co., Ltd

Tel: 0791-83064768 19070905030
Email: cx@chenxisw.com
Country: China
ProdList: 57
Advantage: 58

Dalian Meilun Biotech Co., Ltd.

Tel: 0411-62910999 13889544652
Email: sales@meilune.com
Country: China
ProdList: 4647
Advantage: 58

J & K SCIENTIFIC LTD.

Tel: 010-82848833 400-666-7788
Fax: 86-10-82849933
Email: jkinfo@jkchemical.com
Country: China
ProdList: 96815
Advantage: 76

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View Lastest Price from WST-8 manufacturers

Henan Fengda Chemical Co., Ltd

Product: WST-8 193149-74-5
Price: US $30.00-0.90/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: g-kg-tons, free sample is available
Release date: 2024-04-26

Hebei Duling International Trade Co. LTD

Product: WST-8 193149-74-5
Price: US $70.00/kg
Min. Order: 1kg
Purity: 99%
Supply Ability: 100 tons
Release date: 2023-02-20

Anhui Zhongda Biotechnology Co., Ltd

Product: WST-8 193149-74-5
Price: US $0.00-0.00/g
Min. Order: 1g
Purity: 99%
Supply Ability: 100000tons
Release date: 2023-08-09

193149-74-5, WST-8Related Search:

(2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt 4-[3-(4-IODOPHENYL)-2-(2,4-DINITROPHENYL)-2H-5-TETRAZOLIO]-1,3-BENZENE DISULFONATE WST-5 (Trifluoromethoxy)benzene 2,3,5-Triphenyltetrazolium chloride Nitrotetrazolium blue chloride 1H-Tetrazole-1-acetic acid Tetrazole Nitrobenzene Nitrogen Methoxy ETHYL-BIS-(SODIUMTETRAZOYLAZO)ACETATE WST-8

2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2h-tetrazolium sodium salt

5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2h-tetrazolium inner salt sodium salt (1:1)

2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monoSodium salt

Cell Proliferation Assay

2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophen

GLT008

SODIUM;4-[2-(2-METHOXY-4-NITROPHENYL)-3-(4-NITROPHENYL)TETRAZOL-2-IUM-5-YL]BENZENE-1,3-DISULFONATE

WST8;WST 8

CS-2725

WST-8 sodium

4-[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate

5-(2,4-Disulfophenyl)-3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazoliuM Inner Salt SodiuM Salt

5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium

Sodium 4-[2-(2-methoxy-4-nitro-phenyl)-3-(4-nitrophenyl)tetrazol-3-ium-5-yl]benzene-1,3-disulfonate

2-(2-Methoxy-4-nitrophenyl)

-2H-tetrazolium Sodium Salt

-5-(2,4-disulfophenyl)

sodium 4-(2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)benzene-1,3-disulfonate

ReadiUse WST-8 *50 mM aqueous solution*

Water-soluble tetrazoliuM -8

)-5-(2,4-disuL

fophenyL

ium Sodium SaL

)-3-(4-nitrophenyL

WST-8

2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfo...

Water soluble four azole nitrogen

2-(3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)-5-sulfobenzenesulfonate, sodium salt

WST-8, (2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium Sodium Salt)

WST-8,inhibit,Inhibitor,WST 8

2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt(WST-8)

193149-74-5

C20H13N6NaO11S2

Aromatics

Heterocycles

Intermediates & Fine Chemicals

Labeling and Diagnostics Reagents

Pharmaceuticals