Description Cell Counting Kit-8 (CCK-8)
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WST-8

Description Cell Counting Kit-8 (CCK-8)
Product Name
WST-8
CAS No.
193149-74-5
Chemical Name
WST-8
Synonyms
2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2h-tetrazolium sodium salt;WST-8;GLT008;CS-2725;fophenyL;WST8;WST 8;WST-8 sodium;)-5-(2,4-disuL;ium Sodium SaL;CAS:193149-74-5
CBNumber
CB4547211
Molecular Formula
C20H15N6NaO11S2
Formula Weight
602.48
MOL File
193149-74-5.mol
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WST-8 Property

storage temp. 
under inert gas (nitrogen or Argon) at 2-8°C
solubility 
DMSO: 1 mg/ml; PBS (pH 7.2): 5 mg/ml
form 
A crystalline solid
color 
White to yellow
Appearance
brick solid
InChIKey
FGMWKXXXLURUIM-UHFFFAOYSA-N
SMILES
O(C1C=C(N(=O)=O)C=CC=1[N+]1=NC(C2C=CC(S(O)(=O)=O)=CC=2S([O-])(=O)=O)=NN1C1C=CC(N(=O)=O)=CC=1)C.[NaH]
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H314Causes severe skin burns and eye damage

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P330+P331IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P310Immediately call a POISON CENTER or doctor/physician.

P321Specific treatment (see … on this label).

P363Wash contaminated clothing before reuse.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Cayman Chemical
Product number
18721
Product name
WST-8
Purity
≥98%
Packaging
10mg
Price
$33
Updated
2024/03/01
Cayman Chemical
Product number
18721
Product name
WST-8
Purity
≥98%
Packaging
50mg
Price
$112
Updated
2024/03/01
Cayman Chemical
Product number
18721
Product name
WST-8
Purity
≥98%
Packaging
100mg
Price
$160
Updated
2024/03/01
Cayman Chemical
Product number
18721
Product name
WST-8
Purity
≥98%
Packaging
500mg
Price
$443
Updated
2024/03/01
TRC
Product number
W500038
Product name
WST-8
Packaging
100mg
Price
$250
Updated
2021/12/16
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WST-8 Chemical Properties,Usage,Production

Description

WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt), a highly stable and water-soluble WST, is utilized incell Counting Kit-8 (CCK-8).It is more sensitive than WST-1 particularly at neutralpH. Because of the electron mediator, 1-methoxy PMSin this kit is highly stable, and CCK-8 is stable for at least six months at the room temperature and for one year at 0-5°C (asshown in Figure 1).

Advantages: WST-8 is not cell permeable, which results in low cytotoxicity. Therefore,after the assay, it is possible to continue further experiments using the same cells.Furthermore, it produces the water-soluble formazan upon cellular reduction，which would provide an additional advantage to the method by allowing a simpler assay procedure and not required an extra step to dissolve the formazan.
Disadvantages: An important consideration is that reduction of assay substrates isimpacted by changes in intracellular metabolic activity that has no direct effect on over-all cell viability.

Cell Counting Kit-8 (CCK-8)

Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing the highly water-soluble tetrazolium salt WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2Htetrazolium,monosodium salt] (Patent No. WO97/38985) produces a water-soluble formazan dye upon reduction in the presence of an electron carrier. Cell Counting Kit-8 is a onebottle solution; no premixing of components is required. Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give a yellowcolored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.

Description

WST-8 (Water-Soluble Tetrazolium 8) is a water-soluble tetrazolium salt widely used to assess the metabolic activity of cells. The dye does not penetrate living cells but can be reduced outside the cells by NADPH-dependent cellular oxidoreductases to water-soluble formazan. The reaction occurs by electron transfer across the plasma membrane in a neutral pH and the presence of an intermediate electron acceptor, 1-methoxyphenazine methosulfate. The staining intensity is proportional to the number of viable cells. The maximum absorption of the reaction product is 450–500 nm.


The use of WST-8 has several advantages:


We also supply WST-8 as a ready-to-use kit for cell proliferation assay.

Uses

A highly sensitive tetrazolium reagent that produces a water-soluble formazan (orange color that absorbs at 460 nm) in NADH. It is newer generation formazan-based dyes which release the converted prod uct into the medium in a soluble form allowing for a non-destructive determination of viability enabling the cells to be subject to further investigations.

Uses

WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH.

Biotechnological Applications

WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nM. Useful as a cell viability indicator in cell proliferation assays.

Abs/Em Maxima

458/538 nm

WST-8 Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from WST-8 manufacturers

Shandong Deshang Chemical Co., Ltd.
Product
WST-8 193149-74-5
Price
US $10.00-5.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
10 tons
Release date
2024-08-15
Henan Fengda Chemical Co., Ltd
Product
WST-8 193149-74-5
Price
US $30.00-0.90/KG
Min. Order
1KG
Purity
99%
Supply Ability
g-kg-tons, free sample is available
Release date
2024-04-26
Anhui Zhongda Biotechnology Co., Ltd
Product
WST-8 193149-74-5
Price
US $0.00-0.00/g
Min. Order
1g
Purity
99%
Supply Ability
100000tons
Release date
2023-08-09

193149-74-5, WST-8Related Search:

(2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt 4-[3-(4-IODOPHENYL)-2-(2,4-DINITROPHENYL)-2H-5-TETRAZOLIO]-1,3-BENZENE DISULFONATE WST-5 (Trifluoromethoxy)benzene 2,3,5-Triphenyltetrazolium chloride Nitrotetrazolium blue chloride 1H-Tetrazole-1-acetic acid Tetrazole Nitrobenzene Nitrogen Methoxy ETHYL-BIS-(SODIUMTETRAZOYLAZO)ACETATE WST-8

  • 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2h-tetrazolium sodium salt
  • 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2h-tetrazolium inner salt sodium salt (1:1)
  • 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monoSodium salt
  • Cell Proliferation Assay
  • 2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophen
  • GLT008
  • SODIUM;4-[2-(2-METHOXY-4-NITROPHENYL)-3-(4-NITROPHENYL)TETRAZOL-2-IUM-5-YL]BENZENE-1,3-DISULFONATE
  • WST8;WST 8
  • CS-2725
  • WST-8 sodium
  • 4-[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate
  • 5-(2,4-Disulfophenyl)-3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazoliuM Inner Salt SodiuM Salt
  • 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium
  • Sodium 4-[2-(2-methoxy-4-nitro-phenyl)-3-(4-nitrophenyl)tetrazol-3-ium-5-yl]benzene-1,3-disulfonate
  • 2-(2-Methoxy-4-nitrophenyl)
  • -2H-tetrazolium Sodium Salt
  • -5-(2,4-disulfophenyl)
  • sodium 4-(2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)benzene-1,3-disulfonate
  • ReadiUse WST-8 *50 mM aqueous solution*
  • Water-soluble tetrazoliuM -8
  • )-5-(2,4-disuL
  • fophenyL
  • ium Sodium SaL
  • )-3-(4-nitrophenyL
  • WST-8
  • 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfo...
  • Water soluble four azole nitrogen
  • 2-(3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)-5-sulfobenzenesulfonate, sodium salt
  • WST-8, (2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium Sodium Salt)
  • WST-8,inhibit,Inhibitor,WST 8
  • 2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt(WST-8)
  • 2H-Tetrazolium, 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-, inner salt, sodium salt (1:1)
  • 2-(3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)-5-sulfobenzenesulfonate
  • CAS:193149-74-5
  • 193149-74-5
  • C20H13N6NaO11S2
  • C20H13N6O11S2Na
  • Aromatics
  • Heterocycles
  • Intermediates & Fine Chemicals
  • Labeling and Diagnostics Reagents
  • Pharmaceuticals