Description Cell Counting Kit-8 (CCK-8)
ChemicalBook > CAS DataBase List > WST-8

WST-8

Description Cell Counting Kit-8 (CCK-8)
Product Name
WST-8
CAS No.
193149-74-5
Chemical Name
WST-8
Synonyms
2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2h-tetrazolium sodium salt;WST-8;GLT008;CS-2725;fophenyL;WST8;WST 8;WST-8 sodium;)-5-(2,4-disuL;ium Sodium SaL;)-3-(4-nitrophenyL
CBNumber
CB4547211
Molecular Formula
C20H15N6NaO11S2
Formula Weight
602.48
MOL File
193149-74-5.mol
More
Less

WST-8 Property

storage temp. 
under inert gas (nitrogen or Argon) at 2-8°C
solubility 
DMSO: 1 mg/ml; PBS (pH 7.2): 5 mg/ml
form 
A crystalline solid
More
Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H314Causes severe skin burns and eye damage

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P330+P331IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P310Immediately call a POISON CENTER or doctor/physician.

P321Specific treatment (see … on this label).

P363Wash contaminated clothing before reuse.

P405Store locked up.

P501Dispose of contents/container to..…

More
Less

N-Bromosuccinimide Price

Cayman Chemical
Product number
18721
Product name
WST-8
Purity
≥98%
Packaging
10mg
Price
$33
Updated
2024/03/01
Cayman Chemical
Product number
18721
Product name
WST-8
Purity
≥98%
Packaging
50mg
Price
$112
Updated
2024/03/01
Cayman Chemical
Product number
18721
Product name
WST-8
Purity
≥98%
Packaging
100mg
Price
$160
Updated
2024/03/01
Cayman Chemical
Product number
18721
Product name
WST-8
Purity
≥98%
Packaging
500mg
Price
$443
Updated
2024/03/01
TRC
Product number
W500038
Product name
WST-8
Packaging
100mg
Price
$250
Updated
2021/12/16
More
Less

WST-8 Chemical Properties,Usage,Production

Description

WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt), a highly stable and water-soluble WST, is utilized incell Counting Kit-8 (CCK-8).It is more sensitive than WST-1 particularly at neutralpH. Because of the electron mediator, 1-methoxy PMSin this kit is highly stable, and CCK-8 is stable for at least six months at the room temperature and for one year at 0-5°C (asshown in Figure 1).

Advantages: WST-8 is not cell permeable, which results in low cytotoxicity. Therefore,after the assay, it is possible to continue further experiments using the same cells.Furthermore, it produces the water-soluble formazan upon cellular reduction，which would provide an additional advantage to the method by allowing a simpler assay procedure and not required an extra step to dissolve the formazan.
Disadvantages: An important consideration is that reduction of assay substrates isimpacted by changes in intracellular metabolic activity that has no direct effect on over-all cell viability.

Cell Counting Kit-8 (CCK-8)

Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing the highly water-soluble tetrazolium salt WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2Htetrazolium,monosodium salt] (Patent No. WO97/38985) produces a water-soluble formazan dye upon reduction in the presence of an electron carrier. Cell Counting Kit-8 is a onebottle solution; no premixing of components is required. Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give a yellowcolored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.

Uses

A highly sensitive tetrazolium reagent that produces a water-soluble formazan (orange color that absorbs at 460 nm) in NADH. It is newer generation formazan-based dyes which release the converted prod uct into the medium in a soluble form allowing for a non-destructive determination of viability enabling the cells to be subject to further investigations.

Uses

WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH.

Biotechnological Applications

WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nM. Useful as a cell viability indicator in cell proliferation assays.

WST-8 Preparation Products And Raw materials

Raw materials

Preparation Products

More
Less

WST-8 Suppliers

Canada 1 China 227 India 4 Italy 1 United Kingdom 2 United States 12 Global 247
Nanjing enjing Biotechnology Co., Ltd
Tel
025-86616616 13305189663
Fax
025-86990398
Email
2426321791@qq.com
Country
China
ProdList
1
Advantage
58
Jilin Chinese Academy of Sciences - Yanshen Technology Co., Ltd
Tel
0431-80514535 19917294565
Email
et@chemextension.com
Country
China
ProdList
4108
Advantage
55
Shanghai Better-In Pharmaceutical Technology Co.,Ltd.
Tel
021-38921049 13564871374
Fax
+86-21-38921049
Email
sales@better-in.com
Country
China
ProdList
44
Advantage
55
Shanghai shuji biotechnology co., ltd.
Tel
021-021-62200788 15000569291
Email
zhanlingrui@163.com
Country
China
ProdList
498
Advantage
58
Shandong Kangsaide Biological Technology Co. LTD
Tel
13475906826 18615616891
Email
zhangdong.0602@163.com
Country
China
ProdList
83
Advantage
58
Shandong Zhishang Chemical Co. LTD
Tel
15689709505 15508639737
Email
2138533552@qq.com
Country
China
ProdList
47
Advantage
58
Hubei jian chu biological technology co., LTD
Tel
027-027-65528353 13554206703
Email
3279834739@qq.com
Country
China
ProdList
1075
Advantage
58
Zhangshu Chenxi Biotechnology Co., Ltd
Tel
19070905030
Email
cx@chenxisw.com
Country
China
ProdList
57
Advantage
58
Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
sales@meilune.com
Country
China
ProdList
4664
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
Beijing Ouhe Technology Co., Ltd
Tel
010-82967028 13552068683
Fax
+86-10-82967029
Email
2355560935@qq.com
Country
China
ProdList
12458
Advantage
60
Nanjing Search Biotech Co., Ltd
Tel
+86-025-81682922 +86-13515111588
Fax
025-85860962
Email
sales@searchbio.com.cn
Country
China
ProdList
135
Advantage
68
Nanjing Habo Medical Technology Co., Ltd.
Tel
025-85760892 13376090521
Fax
025-85766769
Email
sales@habotech.com
Country
China
ProdList
996
Advantage
60
Accela ChemBio Co.,Ltd.
Tel
021-50795510 4000665055
Fax
021-50795055
Email
sales@accelachem.com
Country
China
ProdList
11660
Advantage
64
Chemsky (shanghai) International Co.,Ltd
Tel
021-50135380
Email
shchemsky@sina.com
Country
China
ProdList
15421
Advantage
60
Shandong Xiya Chemical Co., Ltd
Tel
13355009207 13355009207
Fax
0539-6365991
Email
3007715519@qq.com
Country
China
ProdList
18739
Advantage
57
Shanghai Sunway Pharmaceutical Technology Co., Ltd
Tel
+8613162137806 18575662672
Fax
021 51613951
Email
mzeng@3wpharm.com
Country
China
ProdList
9743
Advantage
57
Hunan Hui Bai Shi Biotechnology Co., Ltd.
Tel
0731-85526065 13308475853
Email
ivy@hnhbsj.com
Country
China
ProdList
4550
Advantage
62
Chengdu Ai Keda Chemical Technology Co., Ltd.
Tel
4008-755-333 18080918076
Fax
028-86757656
Email
800078821@qq.com
Country
China
ProdList
9725
Advantage
55
9ding chemical ( Shanghai) Limited
Tel
4009209199
Fax
86-021-52271987
Email
sales@9dingchem.com
Country
China
ProdList
22519
Advantage
55
Shanghai Aladdin Bio-Chem Technology Co.,LTD
Tel
18521735133 18521732826;
Fax
021-50323701
Email
market@aladdin-e.com
Country
China
ProdList
25014
Advantage
65
The future of Shanghai Industrial Co., Ltd.
Tel
021-61552785
Fax
021-55660885
Email
sales@shshiji.com
Country
China
ProdList
9552
Advantage
55
Chem-Stone Co.,Ltd.
Tel
020-020-82185713
Fax
020-82185696
Email
sales@chem-stone.com
Country
China
ProdList
741
Advantage
56
TargetMol Chemicals Inc.
Tel
021-021-33632979 15002134094
Fax
021-33632979
Email
marketing@targetmol.com
Country
China
ProdList
7854
Advantage
58
Shanghai DiBai Chemicals Co., Ltd.
Tel
021-54359730 400-008-9730
Fax
021-54353864
Email
info@chemxyz.com
Country
China
ProdList
3993
Advantage
60
Suzhou Lakestar Pharmatech Co., Ltd.
Tel
0512-63970158-603 18018108880
Fax
0512-63971078
Email
info@lakestarpharma.com
Country
China
ProdList
411
Advantage
59
Chengdu HuaXia Chemical Reagent Co. Ltd
Tel
400-1166-196 13458535857
Fax
QQ：800101999
Email
cdhxsj@163.com
Country
China
ProdList
13359
Advantage
58
Suzhou Laing Biological Technology Co., LTD
Tel
0512-62968256 18550502935
Fax
0512-62968256
Email
export@laingbiotec.com
Country
China
ProdList
1043
Advantage
58
Wuhan Pengo Technology.,Ltd.
Tel
027-60707801 13628634921
Email
sunny.w@boronicgroup.com
Country
China
ProdList
175
Advantage
56
Shanghai Macklin Biochemical Co.,Ltd.
Tel
15221275939 15221275939
Fax
021-50706099
Email
shenlinxing@macklin.cn
Country
China
ProdList
15878
Advantage
55
Hangzhou J&H Chemical Co., Ltd.
Tel
+86-571-87396432
Fax
0571-87396431
Email
sales@jhechem.com
Country
China
ProdList
10015
Advantage
53
Nanjing Chalf-Pharm Technology Co., Ltd.
Tel
025-57018978 18251899859
Fax
025-57018008
Email
sales@chalf-pharm.com
Country
China
ProdList
5941
Advantage
50
Shanghai Tombiopharma Chemical Co. Ltd.
Tel
13391076197
Email
tombiopharma@163.com
Country
China
ProdList
4992
Advantage
55
AdooQ Bioscience CHINA
Tel
025-58849295 18951903616;
Fax
025-68650336
Email
info@adooq.cn
Country
China
ProdList
2989
Advantage
60
Taizhou KEDE Chemical Co., Ltd
Tel
0576-84613060 13093829633
Fax
0576-84613060
Email
sales@kedechemical.com
Country
China
ProdList
298
Advantage
60
Hubei Jusheng Technology Co.,Ltd
Tel
027-59599241 18871490274
Fax
027-59599241
Email
1400878000@qq.com
Country
China
ProdList
9972
Advantage
58
Chizhou Kailong Import and Export Trade Co., Ltd.
Tel
Fax
-
Email
xg01_gj@163.com
Country
China
ProdList
9503
Advantage
50
Shanghai Lollane Biological Technology Co.,Ltd.
Tel
021-52996696，15000506266 15000506266
Fax
+86-21-52996696
Country
China
ProdList
4153
Advantage
55
Dalian Idery Biotechnology Co., Ltd.
Tel
0411-62696576 15141125520
Fax
0411-66160116
Email
dlidery@yeah.net
Country
China
ProdList
157
Advantage
58
SanoChem (Chengdu) Tech. Co., Ltd.
Tel
28-87999436 02887999436
Fax
QQ:2746983436
Email
sales@sano-chem.com
Country
China
ProdList
3929
Advantage
55
AOKBIO Inc.
Tel
021-68712331 3286104197
Fax
021 68712331
Country
China
ProdList
323
Advantage
55
Chengdu HuaNa Chemicals Co., Ltd.
Tel
028-86612776; 13699069380
Fax
-
Email
515238037@qq.com
Country
China
ProdList
964
Advantage
55
Shanghai YuanYe Biotechnology Co., Ltd.
Tel
021-61312847; 18021002903
Fax
QQ:3008007432
Email
3008007409@qq.com
Country
China
ProdList
27322
Advantage
60
Shanghai QianYan Bio-technology Co., Ltd
Tel
02781293128
Email
orders@biochemsafebuy.com
Country
China
ProdList
9934
Advantage
55
Shanghai Chaolan Chemical Technology Center
Tel
QQ:65489617 15618227136
Fax
21-5161 9052
Email
info@SuperLan-chem.com
Country
China
ProdList
9756
Advantage
58
Zhengzhou Acme Chemical Co., Ltd.
Tel
0371-0371-55629727 13323845623
Fax
037155629727
Email
2885676761@qq.com
Country
China
ProdList
9859
Advantage
58
Beijing Solarbio Science & Tecnology Co., Ltd.
Tel
010-50973130 4009686088
Email
3193328036@qq.com
Country
China
ProdList
29796
Advantage
68
Amadis Chemical Company Limited
Tel
571-89925085
Fax
0086-571-89925065
Email
sales@amadischem.com
Country
China
ProdList
131981
Advantage
58
Guangdong wengjiang Chemical Reagent Co., Ltd.
Tel
0751-2886750 13927877953
Fax
0751-2886750
Email
3005811397@qq.com
Country
China
ProdList
13346
Advantage
58
Beijing Jin Ming Biotechnology Co., Ltd.
Tel
010-60605840 18892239720
Fax
010-60605840
Email
psaitong@jm-bio.com
Country
China
ProdList
12308
Advantage
58
More
Less

View Lastest Price from WST-8 manufacturers

Henan Fengda Chemical Co., Ltd
Product
WST-8 193149-74-5
Price
US $30.00-0.90/KG
Min. Order
1KG
Purity
99%
Supply Ability
g-kg-tons, free sample is available
Release date
2024-04-26
Hebei Duling International Trade Co. LTD
Product
WST-8 193149-74-5
Price
US $70.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
100 tons
Release date
2023-02-20
Anhui Zhongda Biotechnology Co., Ltd
Product
WST-8 193149-74-5
Price
US $0.00-0.00/g
Min. Order
1g
Purity
99%
Supply Ability
100000tons
Release date
2023-08-09

193149-74-5, WST-8Related Search:

(2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt 4-[3-(4-IODOPHENYL)-2-(2,4-DINITROPHENYL)-2H-5-TETRAZOLIO]-1,3-BENZENE DISULFONATE WST-5 (Trifluoromethoxy)benzene 2,3,5-Triphenyltetrazolium chloride Nitrotetrazolium blue chloride 1H-Tetrazole-1-acetic acid Tetrazole Nitrobenzene Nitrogen Methoxy ETHYL-BIS-(SODIUMTETRAZOYLAZO)ACETATE WST-8

  • 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2h-tetrazolium sodium salt
  • 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2h-tetrazolium inner salt sodium salt (1:1)
  • 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monoSodium salt
  • Cell Proliferation Assay
  • 2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophen
  • GLT008
  • SODIUM;4-[2-(2-METHOXY-4-NITROPHENYL)-3-(4-NITROPHENYL)TETRAZOL-2-IUM-5-YL]BENZENE-1,3-DISULFONATE
  • WST8;WST 8
  • CS-2725
  • WST-8 sodium
  • 4-[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate
  • 5-(2,4-Disulfophenyl)-3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazoliuM Inner Salt SodiuM Salt
  • 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium
  • Sodium 4-[2-(2-methoxy-4-nitro-phenyl)-3-(4-nitrophenyl)tetrazol-3-ium-5-yl]benzene-1,3-disulfonate
  • 2-(2-Methoxy-4-nitrophenyl)
  • -2H-tetrazolium Sodium Salt
  • -5-(2,4-disulfophenyl)
  • sodium 4-(2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)benzene-1,3-disulfonate
  • ReadiUse WST-8 *50 mM aqueous solution*
  • Water-soluble tetrazoliuM -8
  • )-5-(2,4-disuL
  • fophenyL
  • ium Sodium SaL
  • )-3-(4-nitrophenyL
  • WST-8
  • 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfo...
  • Water soluble four azole nitrogen
  • 2-(3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)-5-sulfobenzenesulfonate, sodium salt
  • WST-8, (2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium Sodium Salt)
  • WST-8,inhibit,Inhibitor,WST 8
  • 2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt(WST-8)
  • 193149-74-5
  • C20H13N6NaO11S2
  • Aromatics
  • Heterocycles
  • Intermediates & Fine Chemicals
  • Labeling and Diagnostics Reagents
  • Pharmaceuticals