Nitrotetrazolium blue chloride
- Product Name
- Nitrotetrazolium blue chloride
- CAS No.
- 298-83-9
- Chemical Name
- Nitrotetrazolium blue chloride
- Synonyms
- NBT;NITRO BLUE TETRAZOLIUM;NITRO BLUE TETRAZOLIUM CHLORIDE;NITRO BT;P-NBT;P-NITROTETRAZOLIUM BLUE;P-NITRO BLUE TETRAZOLIUM;4-NITRO BLUE TETRAZOLIUM CHLORIDE;Nitrotetrazolium blue chloride(NBT);NBTMF
- CBNumber
- CB8465489
- Molecular Formula
- C40H30ClN10O6+
- Formula Weight
- 782.19
- MOL File
- 298-83-9.mol
Nitrotetrazolium blue chloride Property
- Melting point:
- 200°C
- Density
- 1.5521 (rough estimate)
- refractive index
- 1.7350 (estimate)
- storage temp.
- 2-8°C
- solubility
- methanol: 50 mg/mL, clear, very strongly yellow
- form
- Powder/Solid
- color
- Yellow
- Water Solubility
- Soluble in water, ethanol and methanol.
- Sensitive
- Light Sensitive
- λmax
- 256 nm
- BRN
- 4115923
- Stability:
- Stable, but store cold.
- Biological Applications
- Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting
- EPA Substance Registry System
- 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride (298-83-9)
Safety
- Hazard Codes
- Xn,Xi
- Risk Statements
- 22-68/20/21/22-20/21/22-36-36/37/38-41
- Safety Statements
- 36-45-36/37-26-39
- WGK Germany
- 3
- RTECS
- XF8045000
- F
- 8-10-23
- TSCA
- Yes
- HS Code
- 32041900
- Toxicity
- LD50 orl-mus: 2 g/kg YAKUD5 32,614,1990
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H332Harmful if inhaled
- Precautionary statements
-
P201Obtain special instructions before use.
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P308+P313IF exposed or concerned: Get medical advice/attention.
N-Bromosuccinimide Price
- Product number
- N5514
- Product name
- Nitro Blue Tetrazolium
- Purity
- tablet
- Packaging
- 10tablets
- Price
- $88.5
- Updated
- 2024/03/01
- Product number
- N5514
- Product name
- Nitro Blue Tetrazolium
- Purity
- tablet
- Packaging
- 25tablets
- Price
- $173
- Updated
- 2024/03/01
- Product number
- 484235
- Product name
- Nitrotetrazolium Blue chloride
- Packaging
- 250mg
- Price
- $85.2
- Updated
- 2024/03/01
- Product number
- 484235
- Product name
- p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem
- Purity
- NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC?? = 3-4 μM).
- Packaging
- 1g
- Price
- $245
- Updated
- 2024/03/01
- Product number
- 11585029001
- Product name
- NBT
- Purity
- 4-Nitro blue tetrazolium chloride, crystals
- Packaging
- 5G
- Price
- $742
- Updated
- 2024/03/01
Nitrotetrazolium blue chloride Chemical Properties,Usage,Production
Description
Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.
Chemical Properties
NBT is a yellow crystals that can soluble in water, aqueous buffers, and dimethylformamide. Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology.
Uses
Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.
Application
Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
It has been used as a substrate for western blot detection.
It has been used for the staining of histological tissue sections.
Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease.
It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase.
Further, it is used in immunology for detection of alkaline phosphatase.
Definition
ChEBI: Nitro blue tetrazolium dichloride is an organic chloride salt having nitro blue tetrazolium(2+) as the counterion. It contains a nitro blue tetrazolium(2+).
General Description
Nitrotetrazolium Blue chloride (NBT) is a colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.
Hazard
Moderately toxic by ingestion.
Biochem/physiol Actions
Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.
Safety Profile
Moderately toxic by ingestion. When heated to decomposition it emits toxic vapors of NOx and Clí.
storage
+4°C
Nitrotetrazolium blue chloride Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from Nitrotetrazolium blue chloride manufacturers
- Product
- Nitrotetrazolium blue chloride 298-83-9
- Price
- US $0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 3500kg/month
- Release date
- 2021-09-14
- Product
- Nitrotetrazolium blue chloride 298-83-9
- Price
- US $999.00-666.00/kg
- Min. Order
- 1kg
- Purity
- 99%
- Supply Ability
- 5000
- Release date
- 2024-08-20
- Product
- Nitrotetrazolium blue chloride 298-83-9
- Price
- US $10.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 10 mt
- Release date
- 2023-01-10