Nitrotetrazolium blue chloride

Product Name: Nitrotetrazolium blue chloride
CAS No.: 298-83-9
Chemical Name: Nitrotetrazolium blue chloride
Synonyms: NBT;NITRO BLUE TETRAZOLIUM;NITRO BLUE TETRAZOLIUM CHLORIDE;NITRO BT;P-NBT;P-NITROTETRAZOLIUM BLUE;P-NITRO BLUE TETRAZOLIUM;4-NITRO BLUE TETRAZOLIUM CHLORIDE;Nitrotetrazolium blue chloride(NBT);NBTMF
CBNumber: CB8465489
Molecular Formula: C40H30ClN10O6+
Formula Weight: 782.19
MOL File: 298-83-9.mol

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Nitrotetrazolium blue chloride Property

Melting point:: 200°C
Density: 1.5521 (rough estimate)
refractive index: 1.7350 (estimate)
storage temp.: 2-8°C
solubility: methanol: 50 mg/mL, clear, very strongly yellow
form: Powder/Solid
color: Yellow
Water Solubility: Soluble in water, ethanol and methanol.
Sensitive: Light Sensitive
λmax: 256 nm
BRN: 4115923
Stability:: Stable, but store cold.
Biological Applications: Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting
EPA Substance Registry System: 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride (298-83-9)

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Safety

Hazard Codes: Xn,Xi
Risk Statements: 22-68/20/21/22-20/21/22-36-36/37/38-41
Safety Statements: 36-45-36/37-26-39
WGK Germany: 3
RTECS: XF8045000
F: 8-10-23
TSCA: Yes
HS Code: 32041900
Toxicity: LD50 orl-mus: 2 g/kg YAKUD5 32,614,1990

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H332Harmful if inhaled

Precautionary statements

P201Obtain special instructions before use.

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: N5514
Product name: Nitro Blue Tetrazolium
Purity: tablet
Packaging: 10tablets
Price: $88.5
Updated: 2024/03/01

Sigma-Aldrich

Product number: N5514
Product name: Nitro Blue Tetrazolium
Purity: tablet
Packaging: 25tablets
Price: $173
Updated: 2024/03/01

Sigma-Aldrich

Product number: 484235
Product name: Nitrotetrazolium Blue chloride
Packaging: 250mg
Price: $85.2
Updated: 2024/03/01

Sigma-Aldrich

Product number: 484235
Product name: p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem
Purity: NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC?? = 3-4 μM).
Packaging: 1g
Price: $245
Updated: 2024/03/01

Sigma-Aldrich

Product number: 11585029001
Product name: NBT
Purity: 4-Nitro blue tetrazolium chloride, crystals
Packaging: 5G
Price: $742
Updated: 2024/03/01

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Nitrotetrazolium blue chloride Chemical Properties,Usage,Production

Description

Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.

Chemical Properties

NBT is a yellow crystals that can soluble in water, aqueous buffers, and dimethylformamide. Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology.

Uses

Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.

Application

Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
It has been used as a substrate for western blot detection.
It has been used for the staining of histological tissue sections.
Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease.
It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase.
Further, it is used in immunology for detection of alkaline phosphatase.

Definition

ChEBI: Nitro blue tetrazolium dichloride is an organic chloride salt having nitro blue tetrazolium(2+) as the counterion. It contains a nitro blue tetrazolium(2+).

General Description

Nitrotetrazolium Blue chloride (NBT) is a colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

Hazard

Moderately toxic by ingestion.

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

Safety Profile

Moderately toxic by ingestion. When heated to decomposition it emits toxic vapors of NOx and Clí.

storage

+4°C

Nitrotetrazolium blue chloride Preparation Products And Raw materials

Raw materials

3,3'-Dimethoxybenzidine dihydrochloride Isoamyl nitrite Ethanol Benzaldehyde Tetrahydrofuran 4-Nitrophenylhydrazine

Preparation Products

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Nitrotetrazolium blue chloride Suppliers

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View Lastest Price from Nitrotetrazolium blue chloride manufacturers

HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD

Product: Nitrotetrazolium blue chloride 298-83-9
Price: US $999.00-666.00/kg
Min. Order: 1kg
Purity: 99%
Supply Ability: 5000
Release date: 2024-08-20

WUHAN FORTUNA CHEMICAL CO., LTD

Product: Nitrotetrazolium blue chloride 298-83-9
Price: US $0.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 3500kg/month
Release date: 2021-09-14

Hebei Weibang Biotechnology Co., Ltd

Product: Nitrotetrazolium blue chloride 298-83-9
Price: US $10.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 10 mt
Release date: 2023-01-10

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Nsc 27622

P- nitrotetrazolium Blue Nitro Blue Tetrazolium (nbt)

ium BL

NitrotetrazoL

ue chL