ChemicalBook > CAS DataBase List > Nitrotetrazolium blue chloride

Nitrotetrazolium blue chloride

Product Name
Nitrotetrazolium blue chloride
CAS No.
298-83-9
Chemical Name
Nitrotetrazolium blue chloride
Synonyms
NBT;NITRO BLUE TETRAZOLIUM;NITRO BLUE TETRAZOLIUM CHLORIDE;NITRO BT;P-NBT;P-NITROTETRAZOLIUM BLUE;P-NITRO BLUE TETRAZOLIUM;4-NITRO BLUE TETRAZOLIUM CHLORIDE;Nitrotetrazolium blue chloride(NBT);NBTMF
CBNumber
CB8465489
Molecular Formula
C40H30ClN10O6+
Formula Weight
782.19
MOL File
298-83-9.mol
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Nitrotetrazolium blue chloride Property

Melting point:
200°C
Density 
1.5521 (rough estimate)
refractive index 
1.7350 (estimate)
storage temp. 
2-8°C
solubility 
methanol: 50 mg/mL, clear, very strongly yellow
form 
Powder/Solid
color 
Yellow
Water Solubility 
Soluble in water, ethanol and methanol.
Sensitive 
Light Sensitive
λmax
256 nm
BRN 
4115923
Stability:
Stable, but store cold.
Biological Applications
Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting
EPA Substance Registry System
2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride (298-83-9)
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Safety

Hazard Codes 
Xn,Xi
Risk Statements 
22-68/20/21/22-20/21/22-36-36/37/38-41
Safety Statements 
36-45-36/37-26-39
WGK Germany 
3
RTECS 
XF8045000
F 
8-10-23
TSCA 
Yes
HS Code 
32041900
Toxicity
LD50 orl-mus: 2 g/kg YAKUD5 32,614,1990
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H332Harmful if inhaled

Precautionary statements

P201Obtain special instructions before use.

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
N5514
Product name
Nitro Blue Tetrazolium
Purity
tablet
Packaging
10tablets
Price
$88.5
Updated
2024/03/01
Sigma-Aldrich
Product number
N5514
Product name
Nitro Blue Tetrazolium
Purity
tablet
Packaging
25tablets
Price
$173
Updated
2024/03/01
Sigma-Aldrich
Product number
484235
Product name
Nitrotetrazolium Blue chloride
Packaging
250mg
Price
$85.2
Updated
2024/03/01
Sigma-Aldrich
Product number
484235
Product name
p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem
Purity
NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC?? = 3-4 μM).
Packaging
1g
Price
$245
Updated
2024/03/01
Sigma-Aldrich
Product number
11585029001
Product name
NBT
Purity
4-Nitro blue tetrazolium chloride, crystals
Packaging
5G
Price
$742
Updated
2024/03/01
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Nitrotetrazolium blue chloride Chemical Properties,Usage,Production

Description

Nitro Blue Tetrazolium (NBT) is a chromogenic substrate that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. It is used in the “NBT test” to evaluate the activity of NADPH oxidase in phagocytes, which results in the production of blue reduced NBT formazan in normal cells but not in those from patients with chronic granulomatous disease. NBT can also be used as a chromogenic activity stain for oxidoreductases in gels or solutions. More commonly NBT is often paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) for the colorimetric detection of alkaline phosphatase activity. Alkaline phosphate converts PCIB to a product that reduces NBT to its formazan derivative, resulting in a black-purple precipitate.

Chemical Properties

NBT is a yellow crystals that can soluble in water, aqueous buffers, and dimethylformamide. Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology.

Uses

Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. It can be used to assess cell viability as a function of redox potential. Intense blue dye used in histochemistry determinations. actively respiring cells convert the water-soluble NBT to a deep blue-colored precipitate.

Application

Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
It has been used as a substrate for western blot detection.
It has been used for the staining of histological tissue sections.
Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease.
It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase.
Further, it is used in immunology for detection of alkaline phosphatase.

Definition

ChEBI: Nitro blue tetrazolium dichloride is an organic chloride salt having nitro blue tetrazolium(2+) as the counterion. It contains a nitro blue tetrazolium(2+).

General Description

Nitrotetrazolium Blue chloride (NBT) is a colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

Hazard

Moderately toxic by ingestion.

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

Safety Profile

Moderately toxic by ingestion. When heated to decomposition it emits toxic vapors of NOx and Clí.

storage

+4°C

Nitrotetrazolium blue chloride Preparation Products And Raw materials

Raw materials

3,3'-Dimethoxybenzidine dihydrochloride Isoamyl nitrite Ethanol Benzaldehyde Tetrahydrofuran 4-Nitrophenylhydrazine

Preparation Products

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View Lastest Price from Nitrotetrazolium blue chloride manufacturers

HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD
Product
Nitrotetrazolium blue chloride 298-83-9
Price
US $999.00-666.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
5000
Release date
2024-08-20
WUHAN FORTUNA CHEMICAL CO., LTD
Product
Nitrotetrazolium blue chloride 298-83-9
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
3500kg/month
Release date
2021-09-14
Hebei Weibang Biotechnology Co., Ltd
Product
Nitrotetrazolium blue chloride 298-83-9
Price
US $10.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
10 mt
Release date
2023-01-10

298-83-9, Nitrotetrazolium blue chlorideRelated Search:

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  • 2,2'-BIS(4-NITROPHENYL)-5,5'-DIPHENYL-3,3'-(3,3'-DIMETHOXY-4,4'-DIPHENYLENE)DITETRAZOLIUM CHLORIDE
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  • 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, chloride (1:2)
  • 3,3'-[3,3-Dimethoxy-(1,1-biphenyl)-4,4-diyl]-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchloride]
  • NBTMF
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  • NITRO BLUE TETRAZOLIUM CHLORIDE MONOHYDRATE: TECH., 90%
  • 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy[1,1'- biphenyl]-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl -, dichloride
  • 2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-
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  • p-Nitro-Blue tetrazolium chlori
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  • 2h-tetrazolium, 3,30-(3,30-dimethoxy- 4,40-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl]-, dichloride
  • Tetrazolium, 3,30-(3,30-dimethoxy-4,40-biphenylylene) bis[2-(p-nitrophenyl)-5-phenyl-2h-chloride]
  • 2, 20-bis(p-nitrophenyl)-5,50-diphenyl-3,30-(3,30-dimethoxy- 4,40-biphenylylene)ditetrazolium Chloride
  • 2,20-bis(p-nitrophenyl)- 5,50-diphenyl-3,30-(3,30-dimethoxy-4,40-diphenylene) ditetrazolium Chloride
  • 2,20-di-p-nitrophenyl- 5,50-diphenyl-3,30-bis(3,30-dimethoxy-4,40-biphenylene) ditetrazolium Chloride
  • 2,20-dinitrophenyl-5,50-diphenyl- 3,30-dimethoxy-4,40-diphenylene)ditetrazolium Chloride
  • 3,30-(3,30-dimethoxy-4,40-biphenylylene)bis[2-(p-nitrophenyl)- 5-phenyl-2h-tetrazolium Chloride]
  • 3,30-(3,30- dimethoxy-4,40-diphenylene)bis[2-(p-nitrophenyl)-5- phenyltetrazolium Chloride]
  • Nsc 27622
  • P- nitrotetrazolium Blue Nitro Blue Tetrazolium (nbt)
  • ium BL
  • NitrotetrazoL
  • ue chL