ChemicalBook > CAS DataBase List > 5(6)-Carboxyfluorescein

5(6)-Carboxyfluorescein

Product Name
5(6)-Carboxyfluorescein
CAS No.
72088-94-9
Chemical Name
5(6)-Carboxyfluorescein
Synonyms
5(6)-FAM;DCFA;5(6)-Carboxyfluoresc;5(6)-CARBOXYLUORESCEIN;4(5)-CARBOXYFLUORESCEIN;5(6)-CARBOXYFLUORESCEIN;5(6)-Carboxyfluoresceine];4(5)-Carboxyfluoresceine];Fluoresceincarboxylic Acid;5(6)-CARBOXYFLUORESCEIN 99%
CBNumber
CB5257173
Molecular Formula
C42H24O14
Formula Weight
752.63
MOL File
72088-94-9.mol
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5(6)-Carboxyfluorescein Property

Melting point:
275 °C
Boiling point:
464.9°C (rough estimate)
Density 
1.3418 (rough estimate)
refractive index 
1.6380 (estimate)
storage temp. 
Keep in dark place,Inert atmosphere,Room temperature
solubility 
DMSO (Sparingly), Methanol (Slightly, Heated)
form 
Solid
color 
Light Yellow to Dark Brown
Water Solubility 
slightly soluble
InChIKey
CPRVUBZYHZGASQ-UHFFFAOYSA-N
SMILES
C12(OC(=O)C3C=CC(C(=O)O)=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1.C12(OC(=O)C3C=C(C(=O)O)C=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1
EPA Substance Registry System
Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo- (72088-94-9)
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Safety

Hazard Codes 
Xi,Xn
Risk Statements 
36-36/37/38-20/21/22
Safety Statements 
37/39-26-36-22
WGK Germany 
3
TSCA 
Yes
HS Code 
29322090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H319Causes serious eye irritation

Precautionary statements

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
8.51082
Product name
5(6)-Carboxyfluorescein
Purity
Novabiochem?
Packaging
1g
Price
$58
Updated
2024/03/01
Sigma-Aldrich
Product number
8.51082
Product name
5(6)-Carboxyfluorescein
Purity
Novabiochem?
Packaging
5G
Price
$194
Updated
2024/03/01
Sigma-Aldrich
Product number
21877
Product name
5(6)-Carboxyfluorescein
Purity
BioReagent, suitable for fluorescence, ≥95% (HPLC)
Packaging
1g
Price
$60.9
Updated
2024/03/01
Sigma-Aldrich
Product number
21877
Product name
5(6)-Carboxyfluorescein
Purity
BioReagent, suitable for fluorescence, ≥95% (HPLC)
Packaging
5G
Price
$207
Updated
2024/03/01
Alfa Aesar
Product number
L13439
Product name
5(6)-Carboxyfluorescein, mixture of isomers, 97%
Packaging
250mg
Price
$51.65
Updated
2024/03/01
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5(6)-Carboxyfluorescein Chemical Properties,Usage,Production

Description

5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers.

Chemical Properties

Dark-Orange to Brown Solid

Uses

A popular probe of determination of intracellular pH.

Uses

5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent.

Uses

As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent

Definition

ChEBI: 5(6)-carboxyfluorescein is a commercially available mixture of 5-carboxyfluorescein and 6-carboxyfluorescein isomers. A fluorescent dye, it is commonly used as a tracer agent. It has a role as a tracer and a fluorescent dye. It contains a 5-carboxyfluorescein and a 6-carboxyfluorescein.

Solubility in organics

5(6)-Carboxyfluorescein is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of 5(6)-carboxyfluorescein in these solvents is approximately 5, 0.5, and 1 mg/ml, respectively.

5(6)-Carboxyfluorescein Preparation Products And Raw materials

Raw materials

Preparation Products

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5(6)-Carboxyfluorescein Suppliers

Shanghai Kuokun Biotechnology Co., Ltd
Tel
15900929397
Email
3154863169@qq.com
Country
China
ProdList
170
Advantage
58
Yantai Beite Chemical Technology Co., Ltd.
Tel
13853550371
Email
yang11360@163.com
Country
China
ProdList
25
Advantage
58
Okeanos Tech Co,. Ltd.
Tel
010-62651721 18610486005
Fax
01062651521-808
Email
minghui.yu@okeanos.com.cn
Country
China
ProdList
496
Advantage
60
Suzhouhongxiong
Tel
18051745768 18051745768
Email
Suzhouhongxiong@163.com
Country
China
ProdList
100
Advantage
58
changzhou ansciep chemical co., ltd.
Tel
0519-86305871 13961213398;
Fax
0519-86305879
Email
tina@ansciepchem.com
Country
China
ProdList
510
Advantage
58
Shanghai Boyle Chemical Co., Ltd.
Tel
Fax
86-21-57758967
Email
sales@boylechem.com
Country
China
ProdList
2923
Advantage
55
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
4006608290; 18621169109
Fax
86-21-61259102
Email
market03@meryer.com
Country
China
ProdList
40241
Advantage
62
Alfa Aesar
Tel
400-6106006
Fax
021-67582001/03/05
Email
saleschina@alfa-asia.com
Country
China
ProdList
30132
Advantage
84
Energy Chemical
Tel
021-021-58432009 400-005-6266
Fax
021-58436166
Email
sales8178@energy-chemical.com
Country
China
ProdList
44751
Advantage
61
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View Lastest Price from 5(6)-Carboxyfluorescein manufacturers

hebei hongtan Biotechnology Co., Ltd
Product
5(6)-Carboxyfluorescein 72088-94-9
Price
US $30.00/kg
Min. Order
1kg
Purity
98%
Supply Ability
3000kg
Release date
2024-03-22
Henan Fengda Chemical Co., Ltd
Product
5(6)-Carboxyfluorescein 72088-94-9
Price
US $35.00-1.20/kg
Min. Order
1kg
Purity
99%
Supply Ability
g-kg-tons, free sample is available
Release date
2024-04-17
Hebei Yanxi Chemical Co., Ltd.
Product
5(6)-Carboxyfluorescein 72088-94-9
Price
US $40.00-15.00/kg
Min. Order
1kg
Purity
0.99
Supply Ability
10 tons
Release date
2023-09-12

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