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HEPES

Product Name
HEPES
CAS No.
7365-45-9
Chemical Name
HEPES
Synonyms
HEPES BUFFER;4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID;hepes solution;HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID;Biological Buffer;HEPES buffer Solution;4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID;4-(2-hydroxyethyl)piperazin-1-ylethanesulphonic acid;2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID;EPES
CBNumber
CB5408557
Molecular Formula
C8H18N2O4S
Formula Weight
238.3
MOL File
7365-45-9.mol
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HEPES Property

Melting point:
234-238 °C
Boiling point:
408℃[at 101 325 Pa]
Density 
1.07 g/mL at 20 °C
vapor pressure 
0Pa at 25℃
refractive index 
n20/D 1.339
storage temp. 
2-8°C
solubility 
H2O: 1 M at 20 °C, clear, colorless
form 
powder
color 
White
PH
5.0-6.5 (1Maqueous solution)
pka
7.5(at 25℃)
PH Range
7.0 - 7.6
Water Solubility 
Soluble
λmax
λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
Merck 
14,4654
BRN 
883043
Stability:
Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
InChIKey
JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP
-3.85 at 20℃
CAS DataBase Reference
7365-45-9(CAS DataBase Reference)
EPA Substance Registry System
1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
24/25-22-36-26
WGK Germany 
1
RTECS 
TL6809000
TSCA 
Yes
HS Code 
29335995
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
PHG0001
Product name
HEPES
Packaging
1KIT
Price
$978
Updated
2024/03/01
Sigma-Aldrich
Product number
H4034
Product name
HEPES
Purity
BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
Packaging
25KG
Price
$6140
Updated
2024/03/01
Sigma-Aldrich
Product number
H3375
Product name
HEPES
Purity
≥99.5% (titration)
Packaging
25KG
Price
$5870
Updated
2024/03/01
Sigma-Aldrich
Product number
H3375
Product name
HEPES
Purity
≥99.5% (titration)
Packaging
50KG
Price
$11060
Updated
2024/03/01
Sigma-Aldrich
Product number
83264
Product name
HEPES buffer solution
Purity
1?M in H2O
Packaging
100ML
Price
$67.1
Updated
2024/03/01
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HEPES Chemical Properties,Usage,Production

Description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Chemical Properties

White crystalline powder

Uses

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

Uses

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
HEPES has been used as a component of:
Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.
Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.

Uses

HEPES is a biological buffer. It may be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for use with the Folin-Ciocalteu protein assay. 

Definition

ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.

General Description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Biological Activity

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

Biological Activity

Multi purpose buffer used in biological research.

storage

Room temperature

Purification Methods

Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]

HEPES Preparation Products And Raw materials

Raw materials

Preparation Products

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HEPES Suppliers

Americas 1 Australia 1 Belgium 2 Canada 5 China 530 Czech Republic 1 Europe 4 France 4 Germany 16 Hungary 1 India 30 Japan 6 Poland 1 Slovakia 1 South Korea 1 Switzerland 3 The Netherlands 2 United Kingdom 21 United States 106 USA 1 Global 737
Bio-Techne Ltd. (Tocris Bioscience)
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LEHVOSS UK / Gee Lawson Chemical Division
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david.davies@lehvoss.co.uk
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Melford Laboratories Ltd
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Fisher Scientific Uk Ltd.
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NiCHe Materials Ltd.
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Mike.Norman@NiCHeMaterials.com
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Molekula Limited
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Apollo Scientific Ltd
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Discovery Fine Chemicals Ltd.
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Biosynth Carbosynth
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Tocris Bioscience
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Apollo Scientific Ltd.
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Melford Laboratories Ltd.
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Molekula Ltd
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Leancare Ltd.
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CARBONE SCIENTIFIC CO.,LTD
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Eurolabs Limited
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MOLEKULA Ltd.
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Fluorochem Ltd.
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Fisher Scientific UK
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Apin Chemicals Ltd.
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VWR International
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View Lastest Price from HEPES manufacturers

Jinan Finer Chemical Co., Ltd
Product
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid 7365-45-9
Price
US $0.00/Kg/Drum
Min. Order
1KG
Purity
99%
Supply Ability
200mt
Release date
2021-11-24
Hebei Zhuanglai Chemical Trading Co.,Ltd
Product
HEPES 7365-45-9
Price
US $25.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
5000kg/week
Release date
2024-04-26
Watson Biotechnology Co.,Ltd
Product
HEPES 7365-45-9
Price
US $0.00-0.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20MT
Release date
2024-08-22

7365-45-9, HEPESRelated Search:

2-Ethylpiperazine 1-Ethylpiperazine 2-Hydroxyethanesulphonic acid 2-(METHYLSULFONYL)ETHANOL Ethyl 2-(Chlorosulfonyl)acetate N-Aminoethylpiperazine 2-Piperidinoethanol Hydroxyethyl starch Piperazine Sodium 2-bromoethanesulphonate Hydroxyethyl Cellulose Calcium dobesilate 1-(2-Hydroxyethyl)piperazine 2-(2-Aminoethylamino)ethanol Sodium isethionate Folic acid Piperazine citrate Ascoric Acid Hepes Buffer Hepes Buffered Saline Hepes Solution

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  • N-2-HYDROXYETHYLPIPERAZINE-N-2-ETHANSULFONICACID(HEPES)
  • 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsure
  • 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid HEPES
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  • HEPES (4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID)
  • HEPES ULTRAPURE
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  • HEPES, >=99.5% (titration)
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