Hexadecyl trimethyl ammonium bromide Property

Melting point:

248-251 °C(lit.)

Density 

1.1107 (rough estimate)

refractive index 

1.5260 (estimate)

Flash point:

244 °C

storage temp. 

Store below +30°C.

solubility 

H₂O: 50 mg/mL

form 

powder

color 

white

PH

5.0-7.0 (25℃, 0.1M in H2O)

Odor

Odorless

PH Range

5 - 7

Water Solubility 

13 g/L (20 ºC)

Sensitive 

Hygroscopic

λmax

λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.05

Merck 

14,2025

BRN 

3598189

Stability:

Stable. Incompatible with strong oxidizing agents. Protect from moisture.

InChIKey

LZZYPRNAOMGNLH-UHFFFAOYSA-M

CAS DataBase Reference

57-09-0(CAS DataBase Reference)

EPA Substance Registry System

Cetyl trimethyl ammonium bromide (57-09-0)

Safety

Hazard Codes 

Xn,N

Risk Statements 

22-37/38-41-50-50/53-36/37/38-48/22

Safety Statements 

26-39-61-37/39-60-36

RIDADR 

UN 3077 9/PG 3

WGK Germany 

3

RTECS 

BQ7875000

F 

3

TSCA 

Yes

HazardClass 

9

PackingGroup 

III

HS Code 

29239000

Toxicity

LD50 in mice, rats (mg/kg): 32.0, 44.0 i.v. (Isomaa, Bjondahl)

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H318Causes serious eye damage

H335May cause respiratory irritation

H373May cause damage to organs through prolonged or repeated exposure

H410Very toxic to aquatic life with long lasting effects

Precautionary statements

P273Avoid release to the environment.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P314Get medical advice/attention if you feel unwell.

Hexadecyl trimethyl ammonium bromide Chemical Properties,Usage,Production

Chemical Properties

White powder. Soluble in water, alcohol, and chloroform.

Uses

Hexadecyltrimethylammonium Bromide (CTAB) is a cationic surfactant used as a template in the synthesis of Mesoporous γ-Fe2O3.

Uses

cetrimonium bromide is a quaternary ammonium salt that can prevent the proliferation of bacteria, fungi, and yeast in cosmetic preparation, while also act as an emulsifying agent.

Uses

antiinfectant

Uses

A cationic detergent used in the isolation of high molecular weight DNA.

Definition

ChEBI: The organic bromide salt that is the bromide salt of cetyltrimethylammonium; one of the components of the topical antiseptic cetrimide.

General Description

Hexadecyltrimethylammonium bromide is a titrant for the potentiometric titration of perchlorate with ionselective electrodes.

Biochem/physiol Actions

Hexadecyltrimethylammonium bromide also called cetyltrimethylammonium bromide (CTAB), is a cationic surfactant. CTAB is employed for extraction of DNA. CTAB method is efficient, cost and time effective for DNA extraction. CTAB is used as a shaping material and reducing agent for nanoparticle generation.

Purification Methods

Crystallise it from EtOH, EtOH/*benzene or from wet acetone after extracting twice with pet ether. Shake it with anhydrous diethyl ether, filter and dissolve it in a little hot MeOH. After cooling in the refrigerator, the precipitate is filtered off at room temperature and re-dissolved in MeOH. Anhydrous ether is added and, after warming to obtain a clear solution, it is cooled and the crystalline material is collected. [Dearden & Wooley J Phys Chem 91 2404 1987, Hakemi et al. J Am Chem Soc 91 120 1987, Beilstein 4 IV 819.]

References

[1] Liu, Tingting et al. “Hexadecyl trimethyl ammonium bromide assisted growth of NiCo2O4@reduced graphene oxide/ nickel foam nanoneedle arrays with enhanced performance for supercapacitor electrodes?.” RSC Advances 7 (2022): 4029–4041.