Ethylenediaminetetraacetic acid disodium salt

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Ethylenediaminetetraacetic acid disodium salt

Description uses References

Product Name: Ethylenediaminetetraacetic acid disodium salt
CAS No.: 139-33-3
Chemical Name: Ethylenediaminetetraacetic acid disodium salt
Synonyms: EDTA-2NA;DISODIUM EDTA;DISODIUM EDETATE;ETA;EDETATE DISODIUM;EDTA DISODIUM;EDTA DISODIUM SALT;TITRIPLEX III;DISODIUM ETHYLENEDIAMINETETRAACETATE;EDTA NA2
CBNumber: CB8371263
Molecular Formula: C10H14N2Na2O8
Formula Weight: 336.21
MOL File: 139-33-3.mol

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Ethylenediaminetetraacetic acid disodium salt Property

Melting point:: 248 °C (dec.)(lit.)
Boiling point:: >100 °C
Density: 1.01 g/mL at 25 °C
vapor pressure: 0Pa at 25℃
storage temp.: 2-8°C
solubility: H₂O: clear, colorless
form: solution
color: ≤5 (0.5 M)(APHA)
Odor: at 100.00?%. odorless
Water Solubility: Miscible with water.
BRN: 3822669
Stability:: Hygroscopic
LogP: -4.3 at 25℃
CAS DataBase Reference: 139-33-3(CAS DataBase Reference)
EPA Substance Registry System: Ethylenediaminetetraacetic acid disodium salt (139-33-3)

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Safety

Hazard Codes: Xn,C
Risk Statements: 36/38-36/37/38-22-34-21/22
Safety Statements: 26-36-37/39-45-36/37/39-36/37
WGK Germany: 2
RTECS: AH4410000
TSCA: Yes
HS Code: 29224999
Hazardous Substances Data: 139-33-3(Hazardous Substances Data)
Toxicity: mouse,LD50,intraperitoneal,260mg/kg (260mg/kg),Nippon Yakurigaku Zasshi. Japanese Journal of Pharmacology. Vol. 52, Pg. 126S, 1956.

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H303May be harmfulif swallowed

H332Harmful if inhaled

H372Causes damage to organs through prolonged or repeated exposure

H412Harmful to aquatic life with long lasting effects

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P273Avoid release to the environment.

P312Call a POISON CENTER or doctor/physician if you feel unwell.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: E7889
Product name: Ethylenediaminetetraacetic acid disodium salt solution
Purity: for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected
Packaging: 100ml
Price: $75.3
Updated: 2024/03/01

Sigma-Aldrich

Product number: E7889
Product name: Ethylenediaminetetraacetic acid disodium salt solution
Purity: for molecular biology, 0.5?M in H2O, DNase, RNase, NICKase and protease, none detected
Packaging: 1L
Price: $372
Updated: 2024/03/01

Sigma-Aldrich

Product number: 2854
Product name: Ethylenediaminetetraacetic acid disodium salt solution
Purity: 2%, solution
Packaging: 15ml
Price: $62.7
Updated: 2024/03/01

Sigma-Aldrich

Product number: 03690
Product name: Ethylenediaminetetraacetic acid disodium salt solution
Purity: BioUltra, for molecular biology, pH 8.0, ~0.5?M in H2O
Packaging: 100ML
Price: $56.1
Updated: 2024/03/01

Alfa Aesar

Product number: 039781
Product name: Ethylenediaminetetraacetic acid disodium salt, 0.100N (0.050M) Standardized solution
Packaging: 1L
Price: $46.9
Updated: 2024/03/01

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Ethylenediaminetetraacetic acid disodium salt Chemical Properties,Usage,Production

Description

Ethylenediaminetetra-acetic acid (abbreviated as EDTA) is used in several industrial applications attributing to its high ability to bind to most of metal cations. EDTA is produced as several salts, e.g. ethylenediaminetetra-acetic acid disodium salt (EDTAS).
EDTA and its salts are used as chelating agents in cosmetic formulations. EDTAS is a preservative, sequestrant, and stabilizer in foods. EDTA is added to ascorbic acid-disodium benzoate containing soft drinks to mitigate the formation of benzene. EDTA and its salts are used as a component in the production of food-contact paper and paperboard. EDTAS is permitted in the feed and drinking water of animals and/or for the treatment of food-producing animals. In the textile industry, EDTA and its salts prevent metal ion impurities from changing colors of dyed products. In the pulp and paper industry, EDTA and its salts inhibit the ability of metal ions from catalyzing the disproportionation of hydrogen peroxide (a typical bleaching agent). EDTAS is used in synthetic rubber manufacture. EDTAS is also used as a corrosion inhibitor to carbon steel in the industries. As an anticoagulant, EDTAS and tripotassium salts of EDTA are most commonly used.

uses

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions, especially with cations to form an octahedral complex. Ethylenediaminetetraacetic acid disodium salt (EDTA) is a blood anticoagulant and contributes to the pathogenesis of pseudothrombocytopenia. It chelates with calcium in the blood and inhibits clotting and is routinely used in haematological tests. It enhances the antibacterial activity of lysozyme. EDTA used in chelation therapy chelates with calcium and favors dilation of artery, solubilisation of atheromatous plaques in atherosclerotic vascular disease. EDTA chelation therapy may also protect from oxidative damage during blood and lipid peroxidation in liver fibrosis.

References

[1] Victor O. Sheffel (2000) Indirect Food Additives and Polymers: Migration and Toxicology.
[2] Lanigan RS, Yamarik TA (2002) Final report on the safety assessment of EDTA, calcium disodium EDTA, diammonium EDTA, dipotassium EDTA, disodium EDTA, TEA-EDTA, tetrasodium EDTA, tripotassium EDTA, trisodium EDTA, HEDTA, and trisodium HEDTA, Int J Toxicol., 21, 95-142

Chemical Properties

Disodium edetate occurs as a white crystalline, odorless powder with a slightly acidic taste.

Originator

Endrate Disodium,Bersworth,US,1959

Uses

disodium EDTA is a preservative used in concentrations of 0.1 to 0.5 percent.

Uses

Disodium Dihydrogen EDTA is a sequestrant and chelating agent whose complete name is disodium ethylenediamine tetraacetate. it is a nonhygroscopic powder that is colorless, odorless, and tasteless at recommended use levels. A 1% solution has a ph of 4.3–4.7. It is used to control the reaction of trace metals to include calcium and magnesium with other organic and inorganic components in food to prevent deterioration of color, texture, and development of precipitates and to prevent oxidation. Its function is comparable to that of disodium calcium edta.

Uses

Chelating agent (metal); pharmaceutic aid (chelating agent).

Uses

Ethylenediaminetetraacetic acid disodium salt is widely used in textile industry. Usually applied to dissolve limescale. It is applied in textile industry, pulp and paper industry and also in chelation therapy. In cosmetics, it acts as a sequestering agent. It acts as a corrosion inhibitor to carbon steel in the industries. It also acts as a food additive.

Definition

ChEBI: An organic sodium salt that is the anhydrous form of the disodium salt of ethylenediaminetetraacetic acid (EDTA).

Application

Ethylenediaminetetraacetic acid disodium salt dihydrate has been in seed germination trials of plant species and in protein extraction from Moss, Physcomitrella paten. It has also been used in lysis and vacuole buffer for the isolation of vacuoles from Petunia petals.
Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Production Methods

Disodium edetate may be prepared by the reaction of edetic acid and sodium hydroxide.

Manufacturing Process

10 mols of ethylene diamine as a 30% aqueous solution and 4 mols of solid caustic soda are placed in a steam heated kettle supplied with an agitator. 8 mols of sodium cyanide as a concentrated water solution (about 30%) are added and the solution heated to 60°C. About a 10 inch vacuum is applied to bring the liquid to incipient boiling. Formaldehyde (7.5 mols of 37% to 40% aqueous solution) is slowly added, the temperature being held at 60°C, and the solution vigorously stirred. Then, when the evolution of ammonia has substantially stopped, an additional 8 mols of sodium cyanide, followed by 8 mols of formaldehyde are added as before. This is continued until 40 mols of cyanide and 40 mols of formaldehyde have been added. Then at the end about 2 mols more of formaldehyde are added, making 42 mols in all, to remove any last traces of cyanide. About 8 to 10 hours are required to complete the reaction. The resulting product, referred to herein as the crude reaction product, is essentially an aqueous solution of the sodium salt of ethylene diamine tetracetic acid.
To 1,000 g of the crude reaction product are added 264 g of ethylene diamine tetracetic acid. The mixture is preferably heated to incipient boiling to increase the rate of reaction, and then the mixture is allowed to cool and crystallize. The crystals formed are filtered off, washed with the smallest possible amount of ice water, and dried to a constant weight, which is 452 g. A representative sample of the product so prepared showed, upon analysis, 13.26% sodium against a theoretical of 13.70% for the disodium salt. The dialkali salt has a pH of about 5.3 and behaves like a weak acid, displacing CO2 from carbonates and reacting with metals to form hydrogen. It is a white crystalline solid.

brand name

Endrate (Hospira); Sodium Versenate (3M Pharmaceuticals).

Therapeutic Function

Pharmaceutic aid (chelating agent)

General Description

Ethylenediamine tetraacetate (EDTA) is a calcium ion chelator, that has a low molecular mass of 292.24 Da.

Flammability and Explosibility

Non flammable

Pharmaceutical Applications

Disodium edetate is used as a chelating agent in a wide range of pharmaceutical preparations, including mouthwashes, ophthalmic preparations, and topical preparations, typically at concentrations between 0.005 and 0.1% w/v.
Disodium edetate forms stable water-soluble complexes (chelates) with alkaline earth and heavy-metal ions. The chelated form has few of the properties of the free ion, and for this reason chelating agents are often described as ‘removing’ ions from solution, a process known as sequestering. The stability of the metal–edetate complex is dependent on the metal ion involved and the pH.
Disodium edetate is also used as a water softener as it will chelate calcium and magnesium ions present in hard water. It is also used therapeutically as an anticoagulant as it will chelate calcium and prevent the coagulation of blood in vitro. Concentrations of 0.1% w/v are used in small volumes for hematological testing and 0.3% w/v in transfusions.

Biochem/physiol Actions

Ethylenediaminetetraacetic acid disodium salt has the ability to block the binding of vasoactive intestinal peptide to macrophage membranes. It is mainly used in the purification of protein, to remove divalent cations and also to prevent protease activity.

Safety Profile

Poison by intraperitoneal and intravenous routes. Moderately toxic by ingestion. Experimental teratogenic and reproductive effects. Mutation data reported. The calcium disodium salt of EDTA is used as a chelating agent in treating lead poisoning. When heated to decomposition it emits toxic fumes of NOx and NasO.

Safety

Disodium edetate is used widely in topical, oral, and parenteral pharmaceutical formulations; it is used extensively in cosmetic and food products. Disodium edetate and edetate calcium disodium are used in a greater number and variety of pharmaceutical formulations than is edetic acid. Both disodium edetate and edetate calcium disodium are poorly absorbed from the gastrointestinal tract and are associated with few adverse effects when used as excipients in pharmaceutical formulations.
Disodium edetate, trisodium edetate, and edetic acid readily chelate calcium and can, in large doses, cause calcium depletion (hypocalcemia) if used over an extended period of time, or if administered too rapidly by intravenous infusion. If used in preparations for the mouth, they can also leach calcium from the teeth. However, edetate calcium disodium does not chelate calcium.
Disodium edetate should be used with caution in patients with renal impairment, tuberculosis, and impaired cardiac function.
Although disodium edetate is generally considered safe, there have been reports of disodium edetate toxicity in patients receiving chelation therapy.
Nasal formulations containing benzalkonium chloride and disodium edetate, both known to be local irritants, were shown to produce an inflammatory reaction, and microscopic examination showed an extended infiltration of the mucosa by eosinophils, and pronounced atrophy and disorganization of the epithelium, although these effects were subsequently shown to be reversible.
The WHO has set an estimated acceptable daily intake for disodium EDTA in foodstuffs of up to 2.5 mg/kg body-weight
LD50 (mouse, IP): 0.26 g/kg
LD50 (mouse, IV): 0.056 g/kg
LD50 (mouse, OP): 2.05 g/kg
LD50 (rabbit, IV): 0.047 g/kg
LD50 (rabbit, OP): 2.3 g/kg
LD50 (rat, OP): 2.0 g/kg

Veterinary Drugs and Treatments

Edetate Disodium (Sodium EDTA) is a chelating agent that is also used to stop the melting effect of collagenases and proteases on the cornea. EDTA is useful in halting melting through inhibition of matrix metalloproteinases, but is not felt to be useful for melting caused by infectious agents. As the effect of EDTA on metalloproteinases is reversible, it must be administered several times daily to be effective.

storage

Edetate salts are more stable than edetic acid (see also Edetic acid). However, disodium edetate dihydrate loses water of crystallization when heated to 120°C. Aqueous solutions of disodium edetate may be sterilized by autoclaving, and should be stored in an alkali-free container.
Disodium edetate is hygroscopic and is unstable when exposed to moisture. It should be stored in a well-closed container in a cool, dry place.

Incompatibilities

Disodium edetate behaves as a weak acid, displacing carbon dioxide from carbonates and reacting with metals to form hydrogen. It is incompatible with strong oxidizing agents, strong bases, metal ions, and metal alloys.

Regulatory Status

GRAS listed. Included in the FDA Inactive Ingredients Database (inhalations; injections; ophthalmic preparations; oral capsules, solutions, suspensions, syrups, and tablets; rectal topical, and vaginal preparations). Included in nonparenteral and parenteral medicines licensed in the UK. Included in the Canadian List of Acceptable Non-medicinal Ingredients.

Ethylenediaminetetraacetic acid disodium salt Preparation Products And Raw materials

Raw materials

Preparation Products

ribonucleic acid for injection Calcium disodium edetate dihydrate

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View Lastest Price from Ethylenediaminetetraacetic acid disodium salt manufacturers

Hebei Zhuanglai Chemical Trading Co.,Ltd

Product: Disodium EDTA 139-33-3
Price: US $60.00/kg
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Purity: 99
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Release date: 2024-05-17

Hebei Zhuanglai Chemical Trading Co.,Ltd

Product: EDTA-2NA 139-33-3
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Shandong Deshang Chemical Co., Ltd.

Product: Ethylenediaminetetraacetic acid disodium salt 139-33-3
Price: US $10.00-3.00/kg
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Supply Ability: 10 tons
Release date: 2024-07-31

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