MOPSO Property

Melting point:

275-280 °C (dec.)

Density 

1.416±0.06 g/cm3(Predicted)

storage temp. 

Sealed in dry,Room Temperature

solubility 

H₂O: 0.5 M at 20 °C, clear

form 

Crystalline Powder

pka

pK1:6.75 (37°C)

color 

White

PH Range

6.2 - 7.6

Odor

Odorless

Water Solubility 

Water solubility under desired conditions ca.112,6 g/L at 20°C.

BRN 

1109697

InChI

InChI=1S/C7H15NO5S/c9-7(6-14(10,11)12)5-8-1-3-13-4-2-8/h7,9H,1-6H2,(H,10,11,12)

InChIKey

NUFBIAUZAMHTSP-UHFFFAOYSA-N

SMILES

C(N1CCOCC1)C(O)CS(O)(=O)=O

CAS DataBase Reference

68399-77-9(CAS DataBase Reference)

EPA Substance Registry System

4-Morpholinepropanesulfonic acid, .beta.-hydroxy- (68399-77-9)

Safety

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36-37/39

WGK Germany 

1

TSCA 

Yes

HS Code 

29349990

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

MOPSO Chemical Properties,Usage,Production

Description

MOPSO Free Acid is a zwitterionic aminosulfonate buffer with a similar structure to MOPS and a useful pH range of 6.5 - 7.9. It may be used as a buffer component in various biological applications including DNA gel electrophoresis. It has been found that MOPSO buffer combined excellent pH stabilization at pH 7·00 and limited color alteration by testing the color changes of raw sugar solutions so that MOPSO buffer and three glass-fiber filters in series can be used to reduce the effect of pH and turbidity on color measurement of raw sugar solution[1]. The activity of the endo-α-D-mannosidase enzyme in MOPSO buffers at pH 7.0 is increased very much over buffers like HEPES or HEPPS. This buffer also has been employed as a buffer component of charcoal yeast extract medium[2]. However, morpholine family buffers (MES, MOPS, and MOPSO) have been reported to interact with the trivalent metal ions Cr³⁺ and Fe³⁺. Therefore, extensive testing is required before using MAPSO.

Chemical Properties

white crystalline powder

Uses

MOPSO is a buffer that works in the 6-7 pH range. Used in pharmaceutical synthesis.

Uses

MOPSO is a biological buffer also referred to as a second generation “Good′s” buffer which displays improved solubility compared to traditional “Good′s” buffers. The pKa of MOPSO is 6.9 which make it an ideal candidate for buffer formulations which require a pH slightly below physiological to maintain a stable environment in solution. MOPSO is considered to be non-toxic to culture cell lines and provides high-solution clarity.

MOPSO can be used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents.

Definition

ChEBI: 3-(n-morpholino)-2-hydroxypropanesulfonic acid is a member of morpholines.

References

[1] Ahmedna M, et al. Use of filtration and buffers in raw sugar colour measurements. Journal of the Science of Food and Agriculture, 1997; 75: 109-116.   
[2] Taha M, et al. Interactions of Biological Buffers with Macromolecules: The Ubiquitous “Smart” Polymer PNIPAM and the Biological Buffers MES, MOPS, and MOPSO. Macromolecules, 2011; 44: 8575–858.