ChemicalBook > CAS DataBase List > Hoechst 33342

Hoechst 33342

Product Name: Hoechst 33342
CAS No.: 23491-52-3
Chemical Name: Hoechst 33342
Synonyms: 33342;ho342;H33342;HOE 33342;Hoest 33342;Hoechst®HOECHST 33342;hoescht 33342;HOE-33342 3HCL;HOECHST NO 33342
CBNumber: CB9853364
Molecular Formula: C27H28N6O
Formula Weight: 452.55
MOL File: 23491-52-3.mol

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Hoechst 33342 Property

Boiling point:: 725.9±70.0 °C(Predicted)
Density: 1.274±0.06 g/cm3(Predicted)
storage temp.: 2-8°C
solubility: H2O: soluble20mg/mL
form: Yellow solid
pka: 10.97±0.10(Predicted)
color: Brown to breen
Appearance: yellow to brown-green solid, yellow to brown-green solution
Water Solubility: Water : 1 mg/mL (2.21 mM; ultrasonic and warming and heat to 80°C)
BRN: 1234011
InChIKey: PRDFBSVERLRRMY-UHFFFAOYSA-N
SMILES: C1(C2C=C3NC(C4=CC=C(OCC)C=C4)=NC3=CC=2)NC2=CC(N3CCN(C)CC3)=CC=C2N=1
CAS DataBase Reference: 23491-52-3(CAS DataBase Reference)

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Safety

Hazard Codes: Xn,C
Risk Statements: 22-37/38-36/37/38-20/21/22-37-35
Safety Statements: 22-36/37-24/25-36-26-45-36/37/39
WGK Germany: 3
RTECS: DT4200000
F: 3-8-10
HS Code: 32129000
Toxicity: dni-hmn-hla 300 nmol/L CRNGDP 13,2389,1992

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

H341Suspected of causing genetic defects

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 382065
Product name: Bisbenzimide H 33342 Fluorochrome, Trihydrochloride
Purity: Cell-permeable, adenine-thymine-specific fluorescent stain.
Packaging: 100mg
Price: $170
Updated: 2025/07/31

Tocris

Product number: 5117
Product name: Hoechst33342
Purity: ≥98%(HPLC)
Packaging: 50
Price: $54
Updated: 2021/12/16

TRC

Product number: H456605
Product name: Hoechst33342
Packaging: 250mg
Price: $165
Updated: 2021/12/16

Usbiological

Product number: 255461
Product name: Hoechst 33342
Packaging: 50mg
Price: $285
Updated: 2021/12/16

Adipogen Life Sciences

Product number: CDX-B0030-M500
Product name: Hoechst33342
Purity: ≥98%(HPLC)
Packaging: 500mg
Price: $171
Updated: 2021/12/16

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Hoechst 33342 Chemical Properties,Usage,Production

Description

Hoechst 33342 (bisbenzimide, HOE 33342) is a cell-permeant blue-emitting fluorescent dye that binds strongly to adenine-thymine-rich regions in the minor groove of double-stranded DNA. Although Hoechst 33342 can bind to all nucleic acids, AT-rich dsDNA strands enhance its fluorescence considerably.

Hoechst 33342 bound with DNA has excitation/emission maxima at 351/461 nm, respectively. The fluorescence intensity of Hoechst 33342 increases with the pH of the solvent. The unbound dye fluoresces in the 510–540 nm range. The green fluorescence of unbound dye may be observed when an excessive dye concentration is used or the sample is insufficiently washed out. The dye has a considerable Stokes shift between the excitation and emission spectrum, making it worthwhile in multicolor labeling experiments.

The additional ethyl group in Hoechst 33342 renders it more cell-permeant than DAPI and other Hoechst dyes. Hoechst 33342 exhibits a 10-fold greater cell permeability than Hoechst 33258. Hoechst 33342 is also less toxic than DAPI, which ensures a higher viability of stained cells.

Hoechst 33342 is used extensively in fluorescence microscopy and flow cytometry for staining chromosomes and nuclei in live and fixed cells. The dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and cell sorting.

Hoechst 33342 is quenched by bromodeoxyuridine (BrdU), commonly used to detect dividing cells. When BrdU is integrated into DNA, the bromine is supposed to deform the minor groove so that Hoechst dyes cannot reach their optimal binding site. This property of Hoechst 33342 is used to study cell-cycle progression.

The commonly used dye concentration to stain bacteria or eukaryote cells is 0.1–10 μg/mL.

We offer Hoechst 33342 as a powder (1H010), a concentrated 10 mg/mL (2G010), and a ready-to-use (1B410) aqueous solutions.

Chemical Properties

yellow powder

Uses

Hoechst 33342 is an A:T-specific DNA minor groove ligand that is a widely used fluorochrome for visualizing cellular DNA. Excitation of the dye occurs around 350 nm with an emission maxima around 461 nm. Hoechst-33342, unlike other Hoechst dyes, is more lipophillic due to an additional ethyl group. The compound has been observed to displace several known DNA intercalators. Adriamycin has been shown to disrupt the DNA dye update. The compound can be used in sorting living cells based on DNA content by FACS, in the visualization of chromatin distribution in living cells, to detect BrdU incorporation into cells, the visualization of fixed cells by microscopy, and to study the initial stages of apoptosis and cell-cycle distribution. In addition to Hoechst-33342''s fluorescent capability, the

Uses

Hoechst 33342 is useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry. Suitable for vital DNA staining of a variety of cell types and as a probe of membrane permeability in mammalian cells.

Definition

ChEBI: 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole is a bibenzimidazole and a N-methylpiperazine. It has a role as a fluorochrome. It is functionally related to a pibenzimol.

Biological Activity

Cell permeable: yes', 'Primary Target
Adenine-thymine-specific fluorescent stain', 'Product does not compete with ATP.', 'Reversible: no

Safety Profile

Mutation data reported. Whenheated to decomposition it emits toxic vapors of NOx.

Abs/Em Maxima

351 (complex)/461 (complex) nm

Hoechst 33342 Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Hoechst 33342 manufacturers

Career Henan Chemical Co

Product: Hoechst 33342 23491-52-3
Price: US $1.00/kg
Min. Order: 1kg
Purity: 95%-99%
Supply Ability: 100kg
Release date: 2018-12-25

23491-52-3, Hoechst 33342Related Search:

Cy5.5 Hoechst 33258 Benzimidazole Motesanib 2-[(6-Amino-9H-purin-9-yl)methyl]-5-methyl-3-(2-methylphenyl)-4(3H)-quinazolinone NSC 23766 TETRAHYDROCHLORIDE NSC697923 MIF Antagonist, ISO-1 GSK-3β Inhibitor XVIII Pictilisib JNJ-10198409 N-[4-[5-(1,3-BENZODIOXOL-5-YL)-3-METHOXY-1H-1,2,4-TRIAZOL-1-YL]PHENYL]-2-(PHENYLTHIO)ACETAMIDE ERK Inhibitor Compound 23 6-HYDROXY-2,5,7,8-TETRAMETHYLCHROMAN-2-CARBOXYLIC ACID 2'-(4-METHACRYLOXYPHENYL)-5-(4-METHYL-1-PIPER-AZINYL)-2,5'-BIBENZIMIDAZOLYL TRIHYDROCHLORIDE Hoechst 33342 HOECHST 33342, TRIHYDROCHLORIDE, TRIHYDRATE

2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole

2’-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-5’-bi-1h-benzimidazole

CELLSTAIN- HOECHST 33342 SOLUTION

2'-(4'-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bis-1H-benzimidazole trihydrochloride trihydrate

2,5-Bi-1H-benzimidazole, 2-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-

bis-benzimide (ethoxyphenyl form)

bisBenzimide, HOE 33342, Hoechst 33342, 2μ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi-1H-benzimidazole trihydrochloride

2,5'-Bibenzimidazole, 2'-(p-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)

2-[2-(4-Ethoxyphenyl)-3H-benzimidazol-5-yl]-6-(4-methylpiperazin-1-yl)-1H-benzimidazole

Bisbenzimide H33342 Fluorochrome Trihydrochloride 〔HOE 33342〕

Bisbenzimide H33342 Fluorochrome Trihydrochloride DMSO Solution (1mg/ml)

2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide

BISBENZIMIDE H 33342 FLUOROCHROME, TRIHYDROCHLORIDE

BISBENZIMIDE H 33342 TRIHYDROCHLORIDE

BISBENZIMIDE H 33342 FLUOROCHROME

BISBENZIMIDE H 33342, FOR FLUORESCENCE

BISBENZIMIDE H 33342 FLUOROCHROME PACKAG

BISBENZIMIDE HOECHST NO 33342TRIHYDROCHL ORIDE

HOECHST 33342 98%

HOE-33342HCl(Bisbenzamide)

Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - CAS 23491-52-3 - Calbiochem

Hoechst 33342 (bisBenzimide H 33342

2’-(p-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-5’-bibenzimidazole

ho342

2'-(4-ETHOXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5-BI(1H-BENZIMIDAZOLE)

2'-(4-ETHOXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2',5'-BI-1H-BENZIMIDAZOLE 3HCL

2'-(4-ETHOXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI-1H-BENZIMIDAZOLE, 3HCL

2'-(4-ETHOXY-PHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2',5'-BI-1H-BENZIMIDAZOLE TRIHYDROCHLORIDE

2'-(4-ETHOXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI-1H-BENZIMIDAZOLE TRIHYDROCHLORIDE

HOE 33342

HOE-33342 3HCL

HOECHST 33342

HOECHST NO 33342

H33342

BISBENZIMIDE 3HCL

BISBENZIMIDE H 33342

Bis Benzimide Hoechst NO33342

Bis Benzimide Hoechst H 33342

Hoest 33342

BisbenziMide H33342 Trihydrochloride(Hoechst 33342,HOE 33342)

Bisbenzimide,2-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5-bi-1H-benzimidazole,trihydrochloride

2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bi(1H-benzimidazole)

2'-(4-Ethoxyphenyl)-6-(4-methylpiperazine-1-yl)-2,6'-bi[1H-benzimidazole]

Bisbenzimide H<sub>3</sub>3342

Hoechst 33342 fluorescent dye

Hoechst(R) 33342,98%

2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzo[d]imidazole

33342

Hoechst&reg

Hoechst 33342 *CAS 23491-52-3*

33342 BisBenzimide H 33342 trihydrochloride

Hoechst 33342 *UltraPure grade*

Cellstain Hoechst 33342 Solution (1mg/ml H2O)

2,6'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-

Hoechst 33342 solution

hoescht 33342

HOE 33342 trihydrochlorde (Hoechst 33342, HO342)

2'-(4'-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bis-1H-...