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5-Carboxyfluorescein N-succinimidyl ester

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5-Carboxyfluorescein N-succinimidyl ester Basic information

Product Name:
5-Carboxyfluorescein N-succinimidyl ester
Synonyms:
  • carboxyfluorescein-NHS ester
  • 5-CARBOXYFLUORESCEIN, SUCCINIMIDYL ESTER
  • 5-CARBOXYFLUORESCEIN-NHS
  • 5-CARBOXYFLUORESCEIN-N-HYDROXYSUCCINIMIDE ESTER
  • 5-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER
  • 5(6)-FAM-NHS
  • 5-FAM SE, 5-CF SE
  • 2,5-Pyrrolidinedione, 1-[[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]oxy]-
CAS:
92557-80-7
MF:
C25H15NO9
MW:
473.39
Product Categories:
  • marker
  • Fluorescent Labels & Indicators
  • Fluorescein and DerivativesFluorescent Stains
  • Nucleic Acid Stains For Microscopy
  • Biochemicals and Reagents
  • DetectionFluorescent Probes, Labels, Particles and Stains
  • Fluorescent Labels
  • Fluorescent Probes, Labels, Particles and Stains
  • Nucleic Acid Stains
  • FLUOROPHORES
  • FAM
Mol File:
92557-80-7.mol
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5-Carboxyfluorescein N-succinimidyl ester Chemical Properties

Melting point:
>300°C (dec.)
Boiling point:
778.7±70.0 °C(Predicted)
Density 
1.74±0.1 g/cm3(Predicted)
storage temp. 
Inert atmosphere,Store in freezer, under -20°C
solubility 
Aqueous Base (Slightly), DMSO (Slightly), Methanol (Slightly)
pka
9.31±0.20(Predicted)
form 
Solid
color 
Yellow to Orange
Appearance
Solid Powder
BRN 
7865828
InChI
InChI=1S/C25H15NO9/c27-13-2-5-17-19(10-13)33-20-11-14(28)3-6-18(20)25(17)16-4-1-12(9-15(16)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2
InChIKey
GECIDMICWWDIBO-UHFFFAOYSA-N
SMILES
C12(C3=C(C=C(O)C=C3)OC3=C1C=CC(O)=C3)C1=C(C=C(C(ON3C(=O)CCC3=O)=O)C=C1)C(=O)O2
CAS DataBase Reference
92557-80-7(CAS DataBase Reference)
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Safety Information

WGK Germany 
3
8-10-21
HS Code 
29329990

MSDS

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5-Carboxyfluorescein N-succinimidyl ester Usage And Synthesis

Description

5-Carboxyfluorescein N-succinimidyl ester is a green fluorescent probe used for labeling nucleotides and peptides. It also can be used in apillary electrophoresis for fluorescent derivatization.

Chemical Properties

5-Carboxyfluorescein N-succinimidyl ester is Yellow to Orange Solid

Uses

5-Carboxyfluorescein N-succinimidyl ester is one of the most popular green fluorescent reagent used for labelling peptides, proteins and nucleotides

Uses

Amine-reactive derivate of 5-carboxyfluorescein single isomer.

Uses

5-Carboxyfluorescein-N-hydroxysuccinimide Ester is one of the most popular green fluorescent probe used for labeling peptides, proteins and nucleotides. It was used as a reagent in the determination of the intracellular pH in bacteria.

Excitation / Emission Maximum

491 nm/518 nm

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