DL-Dithiothreitol Chemical Properties

Melting point:

41-44 °C(lit.)

Boiling point:

125-130 °C (2 mmHg)

Density 

1.04 g/mL at 20 °C

refractive index 

1.5200 (estimate)

Flash point:

>230 °F

storage temp. 

2-8°C

solubility 

H₂O: 50 mg/mL, clear, colorless

form 

Liquid

color 

White

Water Solubility 

Soluble

Merck 

3376

CAS DataBase Reference

27565-41-9(CAS DataBase Reference)

EPA Substance Registry System

2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-rel-(27565-41-9)

Safety Information

Hazard Codes 

Xn

Risk Statements 

25-36/37/38-22

Safety Statements 

26-45-36

WGK Germany 

3

RTECS 

EK1610000

F 

3-10-16-23

HS Code 

29309090

Toxicity

mouse,LD50,intramuscular,108mg/kg (108mg/kg),BEHAVIORAL: CONVULSIONS OR EFFECT ON SEIZURE THRESHOLD,Journal of Pharmacy and Pharmacology. Vol. 1, Pg. 576, 1949.

MSDS

• Language:English Provider:DL-Dithiothreitol
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

DL-Dithiothreitol Usage And Synthesis

Chemical Properties

White solid

Uses

Reagent for the quantitative reduction of disulfide group. Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues. Cleavage of disulfide bonds in proteins.

Uses

DTT is a cell permeable protective agent for Thiol groups.

Preparation

The preparation of DL-Dithiothreitol is as follows:To a round bottom flask was charged THPP (23mg, 0.11mmol, 1.10eq) and the basic buffer solution [tris-(hydroxymethyl)-aminomethane-CaCl2 based buffer; pH 8.00; 1.0mL]. The reaction mixture was stirred at room temperature for about 5 min and then to this homogenous solution was charged trans-4,5-Dihydroxy-1,2-dithiane (15.2mg, 0.10 mmol, 1.00eq.). The reaction mixture was stirred at room temperature until the reaction was complete (about 30 min). The product was readily identified by 1H-NMR analysis.

Definition

ChEBI: D-1,4-dithiothreitol is a 1,4-dithiothreitol. It is an enantiomer of a L-1,4-dithiothreitol.

Purification Methods

Crystallise DTT from ether and sublime it at 37o/0.005mm. It should be stored at 0o. [Beilstein 1 III 2360.]

DL-Dithiothreitol(27565-41-9)Related Product Information

• 3,3'-Thiodipropionic acid
• Phenothiazine
• 2,2'-Dithiosalicylic acid
• 1,4-Butanediol
• 2,3-Butanediol
• threitol
• 2,2'-Thiodiethanol
• DL-Dithiothreitol
• L-DITHIOTHREITOL
• TRANS-4,5-DIHYDROXY-1,2-DITHIANE
• 1,4-BUTANEDITHIOL
• 3-Mercapto-1,2-propanediol
• Butanethiol
• L-THREITOL
• 1,4-DITHIOTHREITOL-1,1,2,3,4,4-D6
• 1-MERCAPTO-2-PROPANOL
• dl-dithiothreitol-d10
• DL-THREITOL