Avermectin B1a Chemical Properties

Boiling point:

940.9±65.0 °C(Predicted)

Density 

1.24±0.1 g/cm3(Predicted)

storage temp. 

Store at -20°C

solubility 

≥143.4 mg/mL in DMSO; ≥11.86 mg/mL in EtOH with ultrasonic; insoluble in H2O

form 

Powder

pka

12.42±0.70(Predicted)

color 

White to light yellow

Stability:

Hygroscopic

EPA Substance Registry System

Avermectin B1a (65195-55-3)

Safety Information

RIDADR 

2588

HazardClass 

6.1(a)

PackingGroup 

II

Toxicity

LD20 orl-rat: 10 mg/kg JAFCAU 32,94,84

Avermectin B1a Usage And Synthesis

Description

Avermectin B_1a is a macrocyclic lactone disaccharide anthelmintic agent that binds to high and low affinity sites on the mammalian GABA_A receptor. Binding to the high affinity site activates the receptor to increase chloride influx, while binding to the low affinity site blocks the channel. Avermectin B_1a inhibits binding of the glycine receptor antagonist strychnine to membranes and the solubilized receptor from rat spinal cord (K_is = 1.3 and 3.6 μM, respectively). Formulations containing avermectin B_1a (>80%) and avermectin B_1b (~20%; ) are used in insecticides and veterinary anthelmintic formulas as abamectin .

Originator

Abamectin,Yellow River Enterprise Co. (a.k.a Yelori)

Uses

Avermectin B1a is the major component (>80%) of a commercially available anthelmintic used to control parasitic nematodes in livestock. Avermectin B1a contains a secbutyl residue in the 25-position. In vitro and in vivo studies have shown that this analogue is the more potent analogue in the commercial product.

Definition

ChEBI: Avermectin B1a is an avermectin.

Manufacturing Process

1. The contents of a lyophilized tube of Streptomyces avermitilis MA-4680 is transferred aseptically to a 250 ml Erlenmeyer flask containing 305 ml of Medium 1: Dextrose 20 g, Peptone 5 g, Meat Extract 5 g, Primary Yeast 3 g, NaCl 5 g, CaCO3 (after pH adjustment) 3 g, Distilled water 1000 ml, pH 7.0. The inoculated flask is incubated for 3 days at 28°C on a rotary shaking machine at a speed of 220 RPM in a 2 inch radius circular orbit. At the end of this time, a 250 ml Erlenmeyer flask containing 50 ml of Medium 2 [Tomato Paste 20 g, Modified Starch (CPC) 20 g, Primary Yeast 10 g, CoCl2·6H2O 0.005 g, Distilled water 1000 ml, pH 7.2-7.4] is inoculated with a 2 ml sample from the first flask. This flask is incubated for 3 days at 28°C on a rotary shaking machine at a speed of 220 RPM in a 2 inch diameter circular orbit. 50 Ml of the resulting fermentation broth containing C-076 is effective against an N.dubius infection in mice.
2. A lyophilized tube of Streptomyces avermitilis MA-4680 is opened aseptically and the contents suspended in 50 ml of Medium 1 in a 250 ml Erlenmeyer flask. This flask is shaken for 3 days at 28°C on a rotary shaking machine 220 RPM with a 2 inch diameter circular orbit. A 0.2 ml portion of this seed medium is used to inoculate a Slant of Medium 3: Dextrose 10.0 g , Bacto Asparagine 0.5 g, K2HPO4 40.5 g, Bacto Agar 15.0 g , Distilled water 1000 ml, pH 7.0. The inoculated slant medium is incubated at 28°C for 10 days and stored at 4°C until used to inoculate 4 more slants of Medium 3. These slants are incubated in the dark for 8 days. One of these slants is used to inoculate 3 baffled 250 ml Erlenmeyer flasks containing 50 ml of No. 4 Seed Medium: Soluble Starch 10.0 g, Ardamine 5.0 g, NZ Amine E 5.0 g, Beef Extract 3.0 g, MgSO4·7H2O 0.5 g, Cerelose 1.0 g, Na2HPO4 0.190 g, KH2PO4 182 g, CaCO3 0.5 g, Distilled water 1000 ml, pH 7.0-7.2. The seed flasks are shaken for 2 days at 27-28°C on a rotary shaking machine at 220 RPM with a 2 inch diameter circular orbit. The contents of these flasks are pooled and used to inoculate (5% inoculum) baffled 250 ml Erlenmeyer flasks containing 40 ml of various production media. Flasks containing media 2, 5 and 6 are incubated for 4 days at 28°C on a rotary shaking machine at 220 RPM with a 2 inch diameter circular orbit. The resulting broth containing C-076 is then harvested and tested for anthelmintic activity. In all cases 6.2 ml of whole broth and the solids obtained from centrifuging 25 ml of whole broth are fully active against N.dubius helminth infections in mice.
3. The one of the four slants of Medium 3 prepared as in Example 2 is used to inoculate a baffled 250 ml Erlenmeyer flask containing 50 ml of Seed Medium No. 4. The seed flask is shaken for 1 day at 27- 28°C on a rotary shaking machine at 220 RPM with a 2 inch diameter circular orbit. The seed flask is then stored stationary at 4°C until it is ready to be used. The contents of this flask are then used to inoculate (5% inoculum) 20 unbaffled 250 ml Erlenmeyer flasks containing 40 ml of Medium No. 2. After 4 days incubation at 28°C on a rotary shaking machine at 220 RPM with a 2 inch diameter circular orbit, 19 of the flasks are harvested and pooled. The combined fermentation broths containing C-076 are filtered affording 500 ml of filtrate and 84 g of mycelia. 78 G of mycelia are extracted with 150 ml of acetone for ? hour with stirring and the mixture filtered. The filter cake is washed with 50 ml of acetone and the filtrate and washings are combined and concentrated to 46.5 ml 30 Ml of the concentrate is adjusted to pH 4 with dilute hydrochloric acid and extracted 3 times with 30 ml portions of chloroform. The extracts are dried by filtering through dry Infusorial Earth (Super-Cel) combined and concentrated to dryness in vacuum. The oily residue of C-076 weighing 91.4 mg is dissolved in chloroform sufficient to make 3 ml of solution which represents 1% of broth volume. The C-076 (Abamectin) obtained in this recovery procedure is fully active against N.dubius infections in mice. In addition, the chloroform extraction achieved a 70 fold purification of C-076 from the whole broth.

brand name

(Merck)Avomec [Veterinary] (Merial); Bovitin [Veterinary] (Merial); Doratect [Veterinary] (Merial); Duomectin [Veterinary] (Merial); Duotin [Veterinary] (Merial); Endecto (Merck); Enzec (Merck); Enzek (Merck); Parafoil (Merck); Vertimil (Zectin (Merck).

Therapeutic Function

Antiparasitic

Hazard

A poison by ingestion. Moderately toxic by skin contact.

Safety Profile

A poison by ingestion.Moderately toxic by skin contact. When heated todecomposition it emits acrid smoke and irritating fumes.

target

insect

Avermectin B1a(65195-55-3)Related Product Information

• avermectin b1b
• Abamectin
• Ivermectin aglycone
• Ivermectin
• :Ivermectin B1a monosaccharide
• 3,4-Dihydro IverMectin
• 28-Oxo IverMectin B1a (IMpurity)
• Avermectin A1a, 5-O-demethyl-22,23-dihydro-
• (4''R)-4''-(ACETYLAMINO)-4''-DEOXY-AVERMECTIN B1
• Emamectin
• Ethyl isocyanoacetate
• 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE
• 25-CYCLOHEXYL-5-O-DEMETHYL-25-DE(1-METHYLPROPYL)AVERMECTIN
• DICHLORO(ETHYLENEDIAMINE)PLATINUM(II)
• PHENYLSELENOL
• Tris(2,4-pentanedionato)chroMiuM(III)
• Cupric acetylacetonate
• TERT-BUTYL ISOCYANIDE
• Abamectin B1a