MCA-LYS-PRO-LEU-GLY-LEU-DAP(DNP)-ALA-ARG-NH2 Chemical Properties

form 

Lyophilized yellow solid.

color 

Light yellow to yellow

Appearance

Lyophilized Yellow Solid

Sequence

{Mca}-Lys-Pro-Leu-Gly-Leu-{Dap(Dnp)}-Ala-Arg-NH2

MCA-LYS-PRO-LEU-GLY-LEU-DAP(DNP)-ALA-ARG-NH2 Usage And Synthesis

Description

Mca-KPLGL-Dpa-AR-NH2 is an N-terminal elongation of the widely used MMP FRET substrate Mca-PLGL-Dpa-AR-NH2 with a Lys yields a fluorogenic peptide with improved substrate properties. Compared with Mca-PLGL-Dpa-AR-NH2, the specificity constant (kcat/Km) of the broad-spectrum MMP-substrate Mca-KPLGL-Dpa-AR-NH2 for collagenases (MMP-1, MMP-8, MMP-13) and MMP-14 is increased by 2-9 times and 3 times, respectively, while those for gelatinases and matrilysin remain equally high.

Uses

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 (FS-6) is a fluorescent peptide that is a quenched MMP peptide substrate. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 can be used for real-time quantification of MMP enzymatic activity. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 is an elongated peptide of MMP substrate (FS-1) and is active against collagenases (MMP-1, MMP-8, MMP-13 ) and MT1-MMP with higher specificity constants than FS-1[1]. (Ex/Em=325 nm/400 nm)

IC 50

MMP-1: 27.5 μM (Km); MMP-8; MMP-13: 5.2 μM (Km); MMP-14: 7.9 μM (Km)

References

[1] Neumann U, et al. Characterization of Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2, a fluorogenic substrate with increased specificity constants for collagenases and tumor necrosis factor converting enzyme. Anal Biochem. 2004 May 15;328(2):166-73. DOI:10.1016/j.ab.2003.12.035

Sequene

Mca-KPLGL-Dpa-AR-NH2

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