Fmoc-4-nitro-L-phenylalanine Chemical Properties

Melting point:

213-223℃

Boiling point:

692.3±55.0 °C(Predicted)

Density 

1.371±0.06 g/cm3(Predicted)

storage temp. 

Sealed in dry,2-8°C

solubility 

almost transparency in N,N-DMF

form 

powder to crystal

pka

3.59±0.10(Predicted)

color 

White to Light yellow

BRN 

5178656

CAS DataBase Reference

95753-55-2(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xi

Safety Statements 

22-24/25

WGK Germany 

3

HS Code 

2924 29 70

HazardClass 

IRRITANT

MSDS

• Language:English Provider:SigmaAldrich

Fmoc-4-nitro-L-phenylalanine Usage And Synthesis

Chemical Properties

White powder

Uses

Fmoc-Phe(4-NO2)-OH is used to prepare squaric acid derivatives as VLA-4 integrin antagonists. It is also an intermediate used in the synthesis of analogs of kahalalide F.

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

Synthesis

GENERAL STEPS: 37.7 g (0.179mol) of (S)-2-amino-3-(4-nitrophenyl)propionic acid was suspended in 200 mL of a 2% NaOH aqueous solution, and 80 mL of acetonitrile was added until completely dissolved. To this solution was added 61.8 g (0.183 mol) of 9-fluorenylmethyl-N-succinimidyl carbonate suspended in 100 mL of acetonitrile. The reaction mixture was stirred at room temperature for 3 h and subsequently acidified to pH 4-5 with 5% HCl. The precipitated white crystals were collected by filtration, washed with ethyl acetate and dried. The product was recrystallized by ethanol-dioxane (50:50) solvent mixture. The yields were 67% (3a) and 71% (3b), respectively. The melting point was 230 °C (literature value 233-234 °C [10]).IR spectrum (ν, cm-1): 3429, 3202, 1724, 1693, 1601, 1520, 1447, 1354, 1223, 1057, 856, 760, 741.1H NMR spectrum (DMSO-d6, δ, ppm): 2.90- 3.09 (m, 1H, CH2), 3.25 (dd, 1H, CH2, 2J=13.7, 3J=4.3 Hz), 4.13-4.34 (m, 4H, 2CH2, 2CH), 7.23-7.45 (m, 4Harom), 7.54 (d, 2Harom, 3J=8.6 Hz), 7.60-7.64 (m, 2Harom), 7.79 (d, 1H, NH, 3J=8.6 Hz), 7.88 (d, 2Harom, 3J=7.5 Hz), 8.14 (d, 2Harom, 3J=8.6 Hz), 12.93 (s, 1H, COOH).13C NMR spectra (DMSO-d6, δ, ppm): 36.62 (CH2) 47.03 (CH), 55.25 (CH), 66.03 (CH2), 120.56, 123.69, 125.62, 127.45, 128.07, 130.94, 141.16, 144.20, 146.73 (arom), 156.39 (CONH), 173.27 (COOH). Mass spectrum (ESI, m/z): 455.1214 [M+Na]+. Molecular formula C24H20N2O6, calculated molecular weight 432.1321.

References

[1] Tetrahedron Letters, 1999, vol. 40, # 36, p. 6557 - 6560
[2] Russian Journal of Organic Chemistry, 2016, vol. 52, # 11, p. 1681 - 1685
[3] Zh. Org. Khim., 2016, vol. 52, # 11, p. 1686 - 1690,5

Fmoc-4-nitro-L-phenylalanine(95753-55-2)Related Product Information

• Fmoc-Phe-OH
• Phenprobamate
• 4-Nitro-3-phenyl-L-alanine
• D-Phenylalanine
• Fmoc-Aib-OH
• 9-Fluorenylmethyl chloroformate
• N-(tert-Butoxycarbonyl)-L-phenylalanine
• L-Phenylalanine
• Fmoc-OSu
• FMOC-L-4-Fluorophe
• Fmoc-L-4-Iodophenylalanine
• Fmoc-Tyr(Me)-OH
• (S)-N-FMOC-4-Chlorophenylalanine
• FMOC-L-9-ANTHRYLALANINE
• FMOC-D, L-PHE(4-C(O)NH2)
• FMOC-L-4-Methylphe
• (S)-N-Fmoc-Allylglycine
• FMOC-L-4-CYANOPHENYLALANINE