Proteinase K

ChemicalBook > CAS DataBase List > Proteinase K

Product Name: Proteinase K
CAS No.: 39450-01-6
Chemical Name: Proteinase K
Synonyms: Protease K;EC 3.4.21.14;Tritirachium album proteinase K;20mg;bum;PROK;40U/MG;CHLOROM;C:C&B53;abs9265
CBNumber: CB1399741
Molecular Formula: C29H27N2O12P
Formula Weight: 626.504641
MOL File: 39450-01-6.mol

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Proteinase K Property

storage temp.: 2-8°C
solubility: H₂O: 10 mg/mL, slightly hazy, brown-yellow
form: powder
color: white
PH: pH7.5～12
Water Solubility: Miscible with water.
InChI: InChI=1/C29H27N2O12P/c1-18(32)23-26(33)30-24(27(34)39-3)25(40-20-10-6-4-7-11-20)28(2,41-21-12-8-5-9-13-21)29(23,30)43-44(37,38)42-22-16-14-19(15-17-22)31(35)36/h4-18,23,32H,1-3H3,(H,37,38)/t18-,23-,28-,29+/s3
InChIKey: CDWGKFQEIBBJOF-BIZWTRPUNA-N
SMILES: N12[C@](OP(O)(OC3=CC=C([N+]([O-])=O)C=C3)=O)([C@H]([C@H](O)C)C1=O)[C@](C)(OC1=CC=CC=C1)C(OC1=CC=CC=C1)=C2C(OC)=O |&1:1,16,17,22,r|
CAS DataBase Reference: 39450-01-6

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Safety

Hazard Codes: Xn
Risk Statements: 36/37/38-42
Safety Statements: 23-24-26-36/37-22-45
WGK Germany: 1
F: 10
HS Code: 35079090

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P284Wear respiratory protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 70663
Product name: Proteinase K, Lyophilized
Purity: Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA.
Packaging: 100mg
Price: $155
Updated: 2024/03/01

Sigma-Aldrich

Product number: 70663
Product name: Proteinase K, Lyophilized
Purity: Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA.
Packaging: 500mg
Price: $506
Updated: 2024/03/01

Sigma-Aldrich

Product number: 539480
Product name: Proteinase K from Tritirachium album
Packaging: 25MG
Price: $58
Updated: 2024/03/01

Sigma-Aldrich

Product number: 539480
Product name: Proteinase K from Tritirachium album
Packaging: 100MG
Price: $132
Updated: 2024/03/01

Alfa Aesar

Product number: J62051
Product name: Proteinase K, Tritirachium album limber
Packaging: 100mg
Price: $109.2
Updated: 2021/12/16

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Proteinase K Chemical Properties,Usage,Production

Description

Proteinase K is a proteolytic enzyme (a serine protease) naturally found in the mold Tritirachium album. In solution, it is stable over a pH range of 4.0–12.5 with an optimum of pH 8.0, and a temperature range of 25°C–65°C. The enzyme has two binding sites for Ca++, though in the absence of this divalent cation some catalytic activity is retained. Maximum proteinase K activity is observed with the inclusion of 1 mM Ca++ in the reaction buffer. Proteinase K digestion is routinely performed at 50°C, and occasionally in the presence of EDTA to inhibit labile, Mg++-dependent nucleases. Proteinase K is prepared commonly as a 20 mg/mL stock solution in sterile water (stable for 1 year at ?20°C) or in a solution of 50 mM Tris, pH 8.0, 1 mM CaCl2 (stable for months at 4°C). It is generally used at a working concentration of up to 50 μg/mL in any of a number of buffer formulations, including those that contain as much as 0.5% SDS[1].

Chemical Properties

Powder beige

Uses

A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines. It finds application in protein modification, determination of enzyme localization and inactivation of RNases/DNases during nucleic acid extraction. It is also used in mitochondria isolation and removal of endotoxins bound to cationic proteins like lysozyme and ribonuclease A. Further, it is involved in the treatment of paraffin embedded tissue sections in order to expose antigen binding sites for antibody labeling.

Uses

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Definition

Proteinase K cleaves peptide bonds next to the carboxyl-terminal of aromatic amino acids, hydrophobic amino acids and sulfuric amino acids, within the polypeptide chain. Hence Proteinase K can act as an endoprotease on a wide range of protein substrates. Because of its proteolytic preferences, it is a member of the subtilisin group of serine proteases. The K in Proteinase K refers to its ability to digest Keratin protein found in hairs, nails and hooves. Proteinase K is an essential reagent in molecular biology labs because it is useful in isolating DNA and RNA molecules by degrading and inactivating proteins. Fungus Tritirachium album Limber naturally produces proteinase K.

General Description

Proteinase K, an extracellular endopeptidase is synthesized by the mold, Tritirachium album Limber. Proteinase K belongs to a new subfamily of the subtilisins. It is a 277 amino acid protein and is characterized with an unhydrolyzed protein chain and autolyzed polypeptide chains.

Biochem/physiol Actions

Proteinase K catalyzes the hydrolysis of keratin.

References

[1] Farrell, R. “Creating a Ribonuclease-Free Environment.”RNA Methodologies (Sixth Edition) 2023: 51-70.

Proteinase K Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Proteinase K manufacturers

Hebei Saisier Technology Co., LTD

Product: Proteinase K 39450-01-6
Price: US $6.00/KG
Min. Order: 1KG
Purity: More than 99%
Supply Ability: 2000KG/Month
Release date: 2024-04-23

Ouhuang Engineering Materials (Hubei) Co., Ltd

Product: Proteinase K 39450-01-6
Price: US $2.00/kg
Min. Order: 1kg
Purity: 99.8%
Supply Ability: 10000ton
Release date: 2024-04-17

Henan Fengda Chemical Co., Ltd

Product: Proteinase K 39450-01-6
Price: US $6.00-0.20/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: g-kg-tons, free sample is available
Release date: 2024-03-28

39450-01-6, Proteinase KRelated Search:

Papain Trypsin EC 3.4.21.19 Pepsin Chymotrypsin ENTEROKINASE Aprotinin Proteinase K Solution DISPASE I EC 3.4.21.14,PROTEINASE 6 FROM ASPERGILLUS SP., ~3 UN ITS/MG EC 3.4.21.14,PROTEINASE, BACTERIAL, ~10 U/MG PROTEINASE FROM BACILLUS LICHENIFORMIS, ~8 U/MG,EC 3.4.21.14 Proteinase, Bacillus subtilis metallo- Acid resistant proteinase neutral proteinase PROTEINASE FROM ASPERGILLUS NIGER >4 U/M,EC 3.4.21.14 EC 3.4.21.14,EC 3.4.21.64 Proteinase, Bacillus subtilis, sutilain

ProteinaseK,PowderAndSolutionAvailable

ProteinaseK,fromTritirachiumalbumLimber

Proteinase K from Tritirachium album ca. 8 DMC-U/mg

PROTEINASEK,LYOPHILIZEDPOWDER,BIOTECHGRADE

Proteinase K for tritirachium album serine.

Protease K

Tritirachium album proteinase K

Proteinase K from Tritirachium album [EC 3.4.21.64] from Tritirachium album

proteinase k for tritirachium album

Proteinase K, tritirachium album serine

Proteinase Ksolution,from Tritirachium album

Einecs 254-457-8

PROTEINASE K FR. TRITIRACHIUMALBUM CA.8 DCM-U/MG LYOPH.

Proteinase K from Tritirachium album,Endopeptidase K

20mg

Proteinase K Solution, 20 mg/ml

40U/MG

Proteinase K froM TritirachiuM albuM ca. 8 DMC-U/Mg lyophil.

RecoMbinant Proteinase K

Proteinase K from Tritirachium album lyophil.

KProteinas

KProteinase K

Proteinase K from Tritirachium album Vetec(TM) reagent grade, powder, >=30 units/mg protein

BOTTLE 1L SMALL BROWN GLASS DIN45

Endopeptidase K Prok Protease K Tritirachium album proteinase K

Proteinase K, for molecular biology, 30 U/mg

Proteinase K39450-01-6

Recombinant Proteinase K (20 mg/ml)

Proteinase K is used to digest protein and remove contamination from preparations of nucleic acid.

IUB: 3.4.21.14

IUB: 3.4.21.64

EC 3.4.21.14

EC 3.4.21.64

ENDOPEPTIDASE K

PROTEINASE K

PROTEINASE K, LYOPHILIZED

PROTEINASE K TRITIRACHIUM ALBUM

PROTEINASE K, IMMOBILIZED

PROK

CHLOROM

NUTRIENT +BLUE +TTC PETRI 90MM (6X20)

Proteinase, Tritirachium album serine

Proteinase K from tritirachium abbum

PROTEINASE K FROM TRITIRACHIUM ALBUM, >500 U/MG*

PROTEINASEK TRITIRACHIUM ALBUM FOR MOLECULAR BIOLOGY, BUFFERED AQUEOUS GLYCEROL SOLUTION, DNASE, NICKASE AND RNASE NONE

PROTEINASE K FROM TRITRACHIUM ALBUM

PROTEINASE K FROM TRITIRACHIUM ALBUM

PROTEINASE K FROM TRITIRACHIUM ALBUM*MOL ECULAR BIOL

PROTEINASE K SOLUTION IN 40%*(V/V) GLYCE ROL

PROTEINASE K FOR TRITIRACHIUM ALBUM, FOR MOL. BIOLOGY

PROTEINASE K FROM TRITIRACHIUM ALBUM 290 U/MG

ProteinaseK(E.C.3.4.21.14)

CT-SMAC (E.COLI 0:157) PETRI 90MM (6X20)

3.4.21.14

Proteinase K from Tritirachium album limber, Recombinant

Native Tritirachium album limber Proteinase K

peptidase K

Native Tritirachium album Proteinase K