DISPASE I
- Product Name
- DISPASE I
- CAS No.
- 42613-33-2
- Chemical Name
- DISPASE I
- Synonyms
- Sarre peptidase;Dispase II protease;Native Bacillus polymyxa Dispase;Dispase from Bacillus polymyxa;Native Bacillus polymyxa Dispase I;Native Bacillus polymyxa Dispase II;Bacillus polymyxa Neutral proteinase;Proteinase, Bacillus polymyxa neutral;Paenibacillus polymyxa neutral protease;Dispase I,Protease from Bacillus polymyxa
- CBNumber
- CB7498033
- Molecular Formula
- NULL
- Formula Weight
- 0
- MOL File
- Mol file
DISPASE I Property
- storage temp.
- 2-8°C
- solubility
- 10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble
Safety
- Hazard Codes
- Xn
- Risk Statements
- 36/37/38-42
- Safety Statements
- 22-26-45-36/37-24
- WGK Germany
- 3
- RTECS
- UK9540000
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H334May cause allergy or asthma symptoms or breathing difficulties if inhaled
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P271Use only outdoors or in a well-ventilated area.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- D4818
- Product name
- Dispase I
- Purity
- protease
- Packaging
- 2mg
- Price
- $206
- Updated
- 2024/03/01
- Product number
- D4693
- Product name
- Dispase II
- Purity
- protease
- Packaging
- 1g
- Price
- $214
- Updated
- 2024/03/01
- Product number
- FD146793
- Product name
- Dispase I
- Packaging
- 2mg
- Price
- $250
- Updated
- 2021/12/16
- Product number
- FD32350
- Product name
- Dispase II
- Packaging
- 1g
- Price
- $310
- Updated
- 2021/12/16
- Product number
- FD146793
- Product name
- Dispase I
- Packaging
- 5mg
- Price
- $500
- Updated
- 2021/12/16
DISPASE I Chemical Properties,Usage,Production
Uses
The enzyme from Sigma has been used in developing a protocol for ex vivo culture of mouse embryonic mammary buds. It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart. It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.
Dispase? II has been used for Fluorescence-Activated Cell Sorting (FACS). It has also been used for separating visceral yolk sac layers.
Uses
Dispase I has been used in a study to assess the effect of amniotic membrane on wound size in the early stages of the healing process. Dispase I has also been used in a study to investigate a dityrosine-based substrate for a protease assay.
Biochem/physiol Actions
Dispase? II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin. Dispase? II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+ activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity. The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.
DISPASE I Preparation Products And Raw materials
Raw materials
Preparation Products
DISPASE I Suppliers
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View Lastest Price from DISPASE I manufacturers
- Product
- Sarre peptidase 42613-33-2
- Price
- US $100.00/KG
- Min. Order
- 1000KG
- Purity
- 99.9%
- Supply Ability
- 100tons
- Release date
- 2021-10-29