EC 3.4.21.19
- Product Name
- EC 3.4.21.19
- CAS No.
- 66676-43-5
- Chemical Name
- EC 3.4.21.19
- Synonyms
- Glu-C;PROTEASE V8;V8 PROTEASE;EC 3.4.21.19;Proteinase V8;V8 proteinase;Glu-C, 500 u/mg;V8 endoprotease;proteinase V8 VAN;PROTEASE, S AUREUS
- CBNumber
- CB2117702
- Molecular Formula
- NULL
- Formula Weight
- 0
- MOL File
- Mol file
EC 3.4.21.19 Property
- storage temp.
- -20°C
- form
- lyophilized powder
- color
- light brown
- Water Solubility
- Soluble in water
Safety
- Hazard Codes
- Xn
- Risk Statements
- 36/37/38-42/43
- Safety Statements
- 22-24-26-36/37-45
- WGK Germany
- 3
- RTECS
- TW0175000
- F
- 10
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H315Causes skin irritation
H317May cause an allergic skin reaction
H319Causes serious eye irritation
H334May cause allergy or asthma symptoms or breathing difficulties if inhaled
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P284Wear respiratory protection.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P405Store locked up.
N-Bromosuccinimide Price
- Product number
- P2922
- Product name
- Endoproteinase Glu-C from Staphylococcus aureus V8
- Purity
- Type XVII-B, lyophilized powder, 500-1,000?units/mg solid
- Packaging
- 100units
- Price
- $57
- Updated
- 2024/03/01
- Product number
- P2922
- Product name
- Endoproteinase Glu-C from Staphylococcus aureus V8
- Purity
- Type XVII-B, lyophilized powder, 500-1,000?units/mg solid
- Packaging
- 500units
- Price
- $192
- Updated
- 2024/03/01
- Product number
- P6181
- Product name
- Endoproteinase Glu-C from Staphylococcus aureus V8
- Purity
- suitable for protein sequencing, lyophilized powder
- Packaging
- 50μg
- Price
- $227
- Updated
- 2024/03/01
- Product number
- P2922
- Product name
- Endoproteinase Glu-C from Staphylococcus aureus V8
- Purity
- Type XVII-B, lyophilized powder, 500-1,000?units/mg solid
- Packaging
- 2500Units
- Price
- $661
- Updated
- 2024/03/01
- Product number
- 324713
- Product name
- Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus
- Packaging
- 50μG
- Price
- $243
- Updated
- 2024/03/01
EC 3.4.21.19 Chemical Properties,Usage,Production
Uses
Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . Product P2922 has been used to linearize cyclic peptides in C. ternatea leaf extract.
Uses
Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for:
- obtaining proteolytic cleavage fragments of the S-layer protein (from Bacillus stearothermophilus ATCC 12980) to perform affinity studies.
- the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination.
- limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase).
- the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.
Biochem/physiol Actions
Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.
EC 3.4.21.19 Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from EC 3.4.21.19 manufacturers
- Product
- EC 3.4.21.19 66676-43-5
- Price
- US $1.00/KG
- Min. Order
- 1KG
- Purity
- 99.0%
- Supply Ability
- 100kg
- Release date
- 2020-01-14