ChemicalBook > CAS DataBase List > Alcohol Oxidase

Alcohol Oxidase

Product Name
Alcohol Oxidase
CAS No.
9073-63-6
Chemical Name
Alcohol Oxidase
Synonyms
EC 1.1.3.13;EC: 1.1.3.13;ALCOHOL OXIDASE;Oxidase, alcohol;ALCOHOL OXIDASE PP;ALCOHOL OXIDASE CB;Alcohol oxidase, solution;ALCOHOL OXIDASE, >400 U/ML*;ALCOHOL:OXYGEN OXIDOREDUCTASE;Alcoholoxidasefrompicheapastoris
CBNumber
CB2465841
Molecular Formula
C10H25NO2
Formula Weight
191.311
MOL File
9073-63-6.mol
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Alcohol Oxidase Property

storage temp. 
-20°C
solubility 
100 mM potassium phosphate, pH 7.5: soluble1.0mg/mL at 25°C (Cold)
form 
vacuum-dried powder
color 
Light yellow to yellow
EPA Substance Registry System
Oxidase, alcohol (9073-63-6)
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Safety

WGK Germany 
3
3-10
HS Code 
35079090
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P285In case of inadequate ventilation wear respiratory protection.

P304+P341IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
A2404
Product name
Alcohol Oxidase solution from Pichia pastoris
Purity
buffered aqueous solution, 10-40?units/mg protein (biuret)
Packaging
250UNITS
Price
$83.3
Updated
2024/03/01
Sigma-Aldrich
Product number
A6941
Product name
Alcohol Oxidase from Candida boidinii
Purity
lyophilized powder, 5-15?units/mg protein
Packaging
50units
Price
$423
Updated
2024/03/01
Sigma-Aldrich
Product number
A2404
Product name
Alcohol Oxidase solution from Pichia pastoris
Purity
buffered aqueous solution, 10-40?units/mg protein (biuret)
Packaging
1000UNITS
Price
$241
Updated
2024/03/01
Sigma-Aldrich
Product number
A6941
Product name
Alcohol Oxidase from Candida boidinii
Purity
lyophilized powder, 5-15?units/mg protein
Packaging
250units
Price
$1410
Updated
2023/01/07
American Custom Chemicals Corporation
Product number
ENZ0000068
Product name
ALCOHOL OXIDASE
Purity
95.00%
Packaging
5MG
Price
$498.66
Updated
2021/12/16
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Alcohol Oxidase Chemical Properties,Usage,Production

Description

Alcohol oxidase (AOX, EC 1.1.3.13) is a flavoenzyme, an oxidoreductase that catalyzes the oxidation of alcohol to the corresponding carbonyl compound and simultaneously releases hydrogen peroxide.
Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to the respective aldehydes.
RCH2OH + O2 -->RCHO + H2O2
The enzyme has the highest affinity for methanol with the affinity decreasing with increasing chain length of the alkyl (R) group.
Alcohol oxidase plays a major role in the metabolism of methanol resulting in the formation of formaldehyde and has been detected in several genera of yeasts, such as Candida, Pichia, and Hansenula, that utilize methanol as a sole carbon and energy source.
Primarily localized in the peroxisome, alcohol oxidase has also been found in the cytoplasm. Monomers are synthesized in the cytosol and assembled into octomers in the peroxisome. Octomerization is thought to be chaparone mediated. Alcohol oxidase is of interest for the study of protein translocation into peroxisomes.

Chemical Properties

Specificity: Alcohol Oxidase is specific for short-chain, linear aliphatic alcohols and oxidizes methanol and ethanol. The following are oxidized at decreasing velocity rate: methanol = ethanol, npropanol, n-butanol. It also catalyzes the oxidation of allyl and propargyl alcohol, methyl and ethyl mercaptan and fromaldehyde at slower rates. Branched chain alcohols, C2-aldehydes or higher, ketones and organic acids are not substrates. However, some of these reagents can give false positive responses due to trace contamination with alcohol. Oxygen is the only known hydrogen acceptor. At air saturation, the apparent KM for alcohol oxidase is 0.7 mM using methanol and 9 mM using ethanol as the substrate. In oxygen saturated solutions the KM values are higher. The turnover number is 20 000 sec-1 .
The optimum temperature range is 40 – 45oC. The pH optimum is 7.2 with a working range of 5.5 – 9.5.
Inactivation: freezing does not inactivate the enzyme. Compared to other oxidases, it is relatively resistant to p-chloromercuribenzoate, heavy metals and hydrogen peroxide. Hydrogen peroxide does not inactivate the enzyme at concentrations many times higher than those reported to inactivate previously-described yeast alcohol oxidases (Hansenula, Candida).
Solubility of the enzyme is inversely related to temperature; refrigeration will often reverse cloudiness in solutions where precipitation is occurring. The enzyme is freely soluble in greater than 0.1 M Phosphate buffer above pH 7.0. The enzyme reversibly precipitates at below 0.05 M Phosphate and below pH 7.0. These boundaries can be extended under certain pH, temperature and ionic conditions. Alcohol Oxidase has a molecular weight of approximately 630 000 and is composed of eight subunits, each having one molecule of bound azide. Inhibitors include azide, Cu++, Ag+ , Hg++ , p-chloromercuribenzoate, hydroxylamine and NaF.

Uses

Alcohol Oxidase may be used to study protein translocation into peroxisomes. Product A2404 is from Pichia pastoris. It has been used for the bacterial expression and immunological verification of Hv-p68 cDNA clones .

Uses

Alcohol oxidase is used to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes . It may be used to study methanol metabolism is yeasts, such as Candida, Pichia, and Hansenula. It is useful to study protein translocation into peroxisomes. Product A6941 is from Candida boidinii and is provided as a lyophilized powder.

Application

Alcohol Oxidase from Hansenula sp. has been used:
in the immobilization on amperometric biosensor by glutaraldehyde cross linking
as a component in pre-mixed hydrogels for the electrode preparation in the biosensors
in the modification of screen printed Co-phthalocyanine/ Carbon electrode for alcohol biosensor preparation

Reactions

In enzymology, an alcohol oxidase (EC 1.1.3.13) is an enzyme that catalyzes the chemical reaction a primary alcohol + O2 ? an aldehyde + H2O2. Thus, the two substrates of this enzyme are primary alcohol and O2, whereas its two products are aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor.

General Description

Alcohol oxidase (AOX) is a homo-octamer, which has a molecular weight of 600-kDa. It has eight flavin adenine dinucleotide (FAD) cofactors.It belongs to the glucose-methanol-choline (GMC) oxidoreductase family.

Biochem/physiol Actions

Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm .

Alcohol Oxidase Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Alcohol Oxidase manufacturers

Henan Fengda Chemical Co., Ltd
Product
Alcohol Oxidase 9073-63-6
Price
US $8.00-6.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
g-kg-tons, free sample is available
Release date
2024-04-23
Career Henan Chemical Co
Product
ALCOHOL OXIDASE 9073-63-6
Price
US $8.00/kg
Min. Order
1kg
Purity
98%
Supply Ability
10MT
Release date
2018-12-16

9073-63-6, Alcohol OxidaseRelated Search:


  • ALCOHOL OXIDASE
  • ALCOHOL OXIDASE CB
  • ALCOHOL OXIDASE PP
  • ALCOHOL:OXYGEN OXIDOREDUCTASE
  • Oxidase, alcohol
  • ALCOHOL OXIDASE FROM PICHIA PASTORIS
  • ALCOHOL OXIDASE FROM CANDIDA BOIDINII
  • ALCOHOL OXIDASE FROM HANSENULA SP.
  • ALCOHOL OXIDASE, >400 U/ML*
  • Alcoholoxidasefrompicheapastoris
  • alcohol oxidase solution from pichia pastoris
  • ALCOHOL OXIDASE, SOLUTION, FROM PICHEA PASTORIS
  • Alcohol Oxidase solution from Pichia pastoris,Alcohol:oxygen oxidoreductase
  • EC 1.1.3.13
  • EC: 1.1.3.13
  • Alcohol oxidase, solution
  • Native Pichia pastoris Alcohol Oxidase
  • Native Candida sp. Alcohol oxidase
  • Native Hansenula sp. Alcohol Oxidase
  • Alcohol Oxidase from Hansenula sp. vacuum-dried powder, >=0.6 units/mg solid
  • 9073-63-6
  • BioChemical
  • Biochemicals and Reagents
  • Application Index
  • Enzymes, Inhibitors, and Substrates
  • Diagnostic and Analytical Enzymes